{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T11:02:17Z","timestamp":1768647737335,"version":"3.49.0"},"reference-count":22,"publisher":"Georg Thieme Verlag KG","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Pediatr Surg"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The aim of this study is to explore the protective effects and mechanisms of Eupatilin, a peroxisome proliferator-activated receptor \u03b1 (PPAR\u03b1) agonist, on cholestatic liver disease induced by common bile duct ligation (BDL) in mice.<\/jats:p>\n                  <jats:p>We selected Balb\/c mice (both male and female) aged 6 to 8 weeks for the common BDL procedure (ethical approval number: MUST-FDCT-20241114001). The groups include the BDL group and the BDL+ Eupatilin group, with three mice in each group. Once the mice developed jaundice postsurgery (5 days), they were treated with Eupatilin via gavage at a dosage of 20\u2009mg\/kg daily for a period of 8 days. On day 13, ocular blood was collected, and liver tissues were extracted for histopathological examination with H&amp;E staining, Sirius Red staining, and subsequent RNA sequencing. Statistical differences among the parameters were evaluated using a t-test.<\/jats:p>\n                  <jats:p>Eupatilin reduces the liver weight\/body weight ratio by 41% and ameliorates liver necrosis and fibrosis in Balb\/c mice. It could decrease alanine transaminase ( p\u2009=\u20090.0498), aspartate aminotransferase (p\u2009=\u20090.0077), while maintaining ALB (Albumin) and \u03b3-GT (gamma-glutamyl transferase) within normal ranges. RNA sequencing analysis revealed that antioxidant genes (acetaldehyde dehydrogenase 2 [Aldh2] and superoxide dismutase 1 [Sod1]) might be the targets of Eupatilin action.<\/jats:p>\n                  <jats:p>We found that Eupatilin can upregulate antioxidant genes (Aldh2; p\u2009=\u20090.0107) and Sod1 (p\u2009=\u20090.0208) of Balb\/c mice, thereby ameliorating BDL damage in mice with cholestatic liver disease.<\/jats:p>","DOI":"10.1055\/a-2676-2832","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T22:44:45Z","timestamp":1755729885000},"page":"059-064","source":"Crossref","is-referenced-by-count":0,"title":["From Bench to Bedside: Eupatilin Activates Antioxidant Defenses and Reduces Fibrosis in Experimental Cholestasis"],"prefix":"10.1055","volume":"36","author":[{"given":"Wei-Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, School of Pharmacy, Macau University of Science and Technology, Macau SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, School of Pharmacy, Macau University of Science and Technology, Macau SAR, China"},{"name":"Precision Regenerative Medicine Research Centre, Medical Sciences Division, Macau University of Science and Technology, Macau SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul Kwong Hang","family":"Tam","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, School of Pharmacy, Macau University of Science and Technology, Macau SAR, China"},{"name":"Precision Regenerative Medicine Research Centre, Medical Sciences Division, Macau University of Science and Technology, Macau SAR, 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