{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T21:47:41Z","timestamp":1784238461757,"version":"3.55.0"},"reference-count":16,"publisher":"Georg Thieme Verlag KG","issue":"04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Perinatol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aspirin is recommended in high-risk pregnancies for the prevention of preeclampsia\/preterm birth; it is unclear whether aspirin's antiplatelet or anti-inflammatory (or both) effects drive its efficacy, and there are no established markers of therapeutic efficacy for pregnancy-specific outcomes. MicroRNAs (miR) are noncoding posttranscriptional modifiers implicated in preeclampsia\/preterm birth and platelet function. We aimed to evaluate whether selected platelet-associated miRs were associated with platelet inhibition following aspirin use and associated with perinatal outcomes.<\/jats:p>\n                  <jats:p>This is a planned secondary analysis of a cohort of high-risk singleton pregnancies taking aspirin. Participants had bloodwork done in the first trimester, preaspirin, 2 to 4 weeks after aspirin initiation (follow-up 1), and at 28 to 32 weeks of gestation (follow-up 2). We selected six platelet-associated miRs (16, 18a, 126, 155, 181a, and 223) that are also associated with preeclampsia and reported quantity as fold-change from baseline (follow-up\/baseline). We evaluated with univariate analysis the relationship between miR fold-change at follow-up visits 1 and 2 with Platelet Function Assay-100 epinephrine closure time (PFA-100, Siemens), a measure of aspirin response. We also evaluated the relationship between miR fold-change from baseline at each visit and the outcome of (1) hypertensive disorders of pregnancy (HDP): preeclampsia or gestational hypertension and (2) preterm birth\u2009&lt;\u200937 weeks.<\/jats:p>\n                  <jats:p>Of the original cohort, 57 were included in this secondary analysis. The fold-change in miR-223 and miR-18a significantly decreased with increasing PFA-100 epinephrine closure time. Twelve (21%) pregnancies had HDP, and eight (14%) had preterm birth\u2009&lt;\u200937 weeks. Four preterm births were due to severe preeclampsia, and one was preeclampsia without severe features and spontaneous labor. Fold-change in miRs was not associated with HDP, but increased miR-223 at 28 to 32 weeks was significantly associated with an increased risk of preterm birth (odds ratio: 2.77 [1.21\u20136.87]; p\u2009=\u20090.04).<\/jats:p>\n                  <jats:p>Selected platelet-associated miRs may be promising markers to assess the therapeutic effect of aspirin in pregnancy and warrant further exploration.<\/jats:p>","DOI":"10.1055\/a-2677-6668","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T22:56:48Z","timestamp":1756249008000},"page":"557-560","source":"Crossref","is-referenced-by-count":2,"title":["Platelet-Associated microRNAs as Markers of Aspirin Response and Pregnancy Outcome in Pregnancies at High-Risk for Preeclampsia"],"prefix":"10.1055","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3705-6943","authenticated-orcid":false,"given":"Rupsa C.","family":"Boelig","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine, Thomas Jefferson University, Sidney Kimmel Medical College, Philadelphia, Pennsylvania"},{"name":"Department of Pharmacology, Physiology, and Cancer Biology, Thomas Jefferson University, Sidney Kimmel Medical College, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonios","family":"Tawk","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zhan","sequence":"additional","affiliation":[{"name":"Division of Biostatistics, Department of Pharmacology, Physiology, and Cancer Biology, Thomas Jefferson University, Sidney Kimmel Medical College, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter K.","family":"Kraft","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Physiology, and Cancer Biology, Thomas Jefferson University, Sidney Kimmel Medical College, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven E.","family":"McKenzie","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Michael","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"S1108","DOI":"10.1016\/j.ajog.2020.08.045","article-title":"Prevention of preeclampsia with aspirin","volume":"226","author":"D L Rolnik","year":"2022","journal-title":"Am J Obstet Gynecol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3389\/fcvm.2017.00060","article-title":"MicroRNAs: new players in the pathobiology of preeclampsia","volume":"4","author":"K R Bounds","year":"2017","journal-title":"Front Cardiovasc Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.gene.2017.07.010","article-title":"Circulating microRNA expression as predictor of preeclampsia and its severity","volume":"627","author":"D S Jairajpuri","year":"2017","journal-title":"Gene"},{"issue":"08","key":"ref4","doi-asserted-by":"crossref","first-page":"e0160808","DOI":"10.1371\/journal.pone.0160808","article-title":"Systematic review of micro-RNA expression in pre-eclampsia identifies a number of common pathways associated with the disease","volume":"11","author":"A M Sheikh","year":"2016","journal-title":"PLoS 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Chyrchel","year":"2015","journal-title":"Platelets"},{"issue":"02","key":"ref11","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/s41401-018-0041-7","article-title":"Plasma miR-142 predicts major adverse cardiovascular events as an intermediate biomarker of dual antiplatelet therapy","volume":"40","author":"Q J Tang","year":"2019","journal-title":"Acta Pharmacol Sin"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"294954","DOI":"10.1155\/2015\/294954","article-title":"Circulating microRNAs as clinical biomarkers in the predictions of pregnancy complications","volume":"2015","author":"M Tsochandaridis","year":"2015","journal-title":"BioMed Res Int"},{"issue":"S 01","key":"ref13","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/jth.12214","article-title":"MicroRNAs in platelet production and activation","volume":"11","author":"L C Edelstein","year":"2013","journal-title":"J Thromb 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