{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:25:15Z","timestamp":1787498715655,"version":"build-2736575974"},"reference-count":35,"publisher":"Georg Thieme Verlag KG","issue":"10","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation\n                        of China","doi-asserted-by":"crossref","award":["82074221"],"award-info":[{"award-number":["82074221"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Clin Endocrinol Diabetes"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study investigated the organ-specific effects of a high-methionine (HM) diet\n                    in streptozotocin (STZ)-induced diabetic rats, focusing on hepatic and renal\n                    metabolic adaptations. Male Wistar rats were divided into four groups\n                    (n=8\/group): normal control, HM (2% methionine), STZ-diabetic, and HM+STZ. Over\n                    12 weeks, HM supplementation in diabetic rats significantly reduced hepatic\n                    triglyceride accumulation (42.00\u00b17.71 vs. 20.76\u00b13.63 mg\/g tissue, P&lt;0.01),\n                    coinciding with AMP-activated protein kinase (AMPK) activation (1.96-fold,\n                    P&lt;0.05) and downregulation of lipogenic genes (sterol regulatory\n                    element-binding protein 1c \u219363.2%, P&lt;0.05). Conversely, HM exacerbated\n                    diabetic nephropathy, elevating urinary albumin-creatinine ratio (411.90\u00b188.86\n                    vs. 238.41\u00b162.52 mg\/g, P&lt;0.05) and glomerulosclerosis index (2.5\u00b10.5 vs.\n                    1.8\u00b10.4, P&lt;0.001). Hyperhomocysteinemia (105.69\u00b133.81 \u03bcmol\/L) persisted\n                    across HM groups without altering folate\/vitamin B12 levels (P&gt;0.05). These\n                    findings demonstrate a striking dichotomy: HM diet ameliorates hepatic steatosis\n                    through AMPK-mediated lipid modulation while accelerating renal injury via\n                    homocysteine-dependent pathways. The results highlight the need for\n                    organ-specific nutritional strategies in diabetes management.<\/jats:p>","DOI":"10.1055\/a-2686-7562","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T23:34:46Z","timestamp":1761780886000},"page":"492-501","source":"Crossref","is-referenced-by-count":1,"title":["Differential Effects of High Methionine Diet on Biochemical\n                    Parameters in Normal and Diabetic Rat Models"],"prefix":"10.1055","volume":"133","author":[{"given":"Yongwei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meimei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Li","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HaoYan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomu","family":"Kong","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Gao","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GuoXiong","family":"Deng","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Key Lab Immune-Mediated Inflammatory Diseases,\n                    Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing,\n                    China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongtong","family":"Cao","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Lab Immune-Mediated Inflammatory Diseases,\n                    Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing,\n                    China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Ma","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, China-Japan Friendship Hospital,\n                    Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/S1665-2681(19)31355-9","article-title":"S-adenosylmethionine metabolism and liver disease","volume":"12","author":"J M Mato","year":"2013","journal-title":"Ann Hepatol"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2025.05.436","article-title":"Insufficient S-sulfhydration of cAMP-response element binding protein 1\n                    participates in hyperhomocysteinemia or cisplatin induced kidney fibrosis via\n                    promoting epithelial-mesenchymal transition","author":"S Chen","year":"2025","journal-title":"Free Radic Biol Med"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.cld.2022.01.008","article-title":"The interplay between nonalcoholic fatty liver disease and kidney disease","volume":"26","author":"E Truong","year":"2022","journal-title":"Clin Liver Dis"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"81","DOI":"10.2337\/db18-0556","article-title":"SREBP1c-PAX4 axis mediates pancreatic \u03b2-cell compensatory responses upon\n                    metabolic stress","volume":"68","author":"G Lee","year":"2019","journal-title":"Diabetes"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2017.01.012","article-title":"Pleiotropic responses to methionine restriction","volume":"94","author":"GPJJ E Ables","year":"2017","journal-title":"Exp Gerontol"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"2603","DOI":"10.1096\/fj.14-270348","article-title":"UCP1 is an essential mediator of the effects of methionine restriction on energy\n                    balance but not insulin sensitivity","volume":"29","author":"D Wanders","year":"2015","journal-title":"FASEB J"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO02571A","article-title":"Dietary methionine restriction improves glucose metabolism in the skeletal\n                    muscle of obese mice","author":"T Luo","year":"2019","journal-title":"Food Funct"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"F988","DOI":"10.1152\/ajprenal.00195.2023","article-title":"Podocyte-specific silencing of acid sphingomyelinase gene to abrogate\n                    hyperhomocysteinemia-induced NLRP3 inflammasome activation and glomerular\n                    inflammation","volume":"326","author":"D Huang","year":"2024","journal-title":"Am J Physiol Renal Physiol"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"G627","DOI":"10.1152\/ajpgi.00090.2022","article-title":"Hepatocyte- or macrophage-specific SREBP-1a deficiency in mice exacerbates\n                    methionine- and choline-deficient diet-induced nonalcoholic fatty liver\n                    disease","volume":"323","author":"M Araki","year":"2022","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"e3000410","DOI":"10.1371\/journal.pbio.3000410","article-title":"The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research","volume":"18","author":"N Percie du Sert","year":"2020","journal-title":"PLoS Biol"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"4961","DOI":"10.3390\/ijms22094961","article-title":"Methionine diet evoked hyperhomocysteinemia causes hippocampal alterations,\n                    metabolomics plasma changes and behavioral pattern in wild type rats","volume":"22","author":"M Kovalska","year":"2021","journal-title":"Int J Mol Sci"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"230","DOI":"10.7705\/biomedica.v36i2.2686","article-title":"Streptozotocin diabetogenic action in an experimental neonatal induction\n                    model","volume":"36","author":"L Bequer","year":"2016","journal-title":"Biomedica"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1039\/D4FO01613K","article-title":"High methionine intake alters gut microbiota and lipid profile and leads to\n                    liver steatosis in mice","volume":"15","author":"L Zhou","year":"2024","journal-title":"Food Funct"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"702","DOI":"10.1016\/j.cmet.2022.03.010","article-title":"SREBP1c-PARP1 axis tunes anti-senescence activity of adipocytes and ameliorates\n                    metabolic imbalance in obesity","volume":"34","author":"G Lee","year":"2022","journal-title":"Cell Metab"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"3143","DOI":"10.1016\/j.bbagen.2012.09.008","article-title":"Glutathione synthesis","volume":"1830","author":"S C Lu","year":"2013","journal-title":"Biochim Biophys Acta"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-270348","article-title":"UCP1 is an essential mediator of the effects of methionine restriction on energy\n                    balance but not insulin sensitivity","volume":"29","author":"D Wanders","year":"2015","journal-title":"FASEB J"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1097\/IMNA-D-24-00027","article-title":"Kidney fibrosis: Fundamental questions, challenges, and perspectives","volume":"11","author":"Y Liu","year":"2024","journal-title":"Integrative Med Nephrol Androl"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1159\/000101471","article-title":"Podocyte injury and glomerulosclerosis in hyperhomocysteinemic rats","volume":"27","author":"F Yi","year":"2007","journal-title":"Am J Nephrol"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"1356","DOI":"10.1016\/S0006-291X(02)02084-3","article-title":"Smad pathway is activated in the diabetic mouse kidney and Smad3 mediates\n                    TGF-\u03b2-induced fibronectin in mesangial cells","volume":"296","author":"M Isono","year":"2002","journal-title":"Biochem Biophys Res Commun"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"12","DOI":"10.3389\/fnut.2023.1179807","article-title":"Homocysteine-a retrospective and prospective appraisal","volume":"10","author":"A Mccaddon","year":"2023","journal-title":"Front Nutr"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1038\/ki.2009.117","article-title":"The kidney is the major site of S-adenosylhomocysteine disposal in humans","volume":"76","author":"G Garibotto","year":"2009","journal-title":"Kidney Int"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1515\/biol-2021-0054","article-title":"Hyperhomocysteinemia exacerbates ischemia-reperfusion injury-induced acute\n                    kidney injury by mediating oxidative stress, DNA damage, JNK pathway, and\n                    apoptosis","volume":"16","author":"M Zhang","year":"2025","journal-title":"Open Life Sci"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"219","DOI":"10.7150\/ijbs.16725","article-title":"Hyperhomocysteinemia exacerbates cisplatin-induced acute kidney injury","volume":"13","author":"Y Long","year":"2017","journal-title":"Int J Biol Sci"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"115033","DOI":"10.1016\/j.bcp.2022.115033","article-title":"Methionine cycle in nonalcoholic fatty liver disease and its potential\n                    applications","author":"H Y Wang","year":"2022","journal-title":"Biochem Pharmacol"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"109245","DOI":"10.1016\/j.mehy.2019.109245","article-title":"A novel approach in the management of hyperhomocysteinemia","volume":"129","author":"S S Qureshi","year":"2019","journal-title":"Med Hypotheses"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"S20","DOI":"10.2337\/dc24-S002","article-title":"Diagnosis and classification of diabetes: Standards of care in\n                    diabetes-2024","volume":"47","author":"American Diabetes Association Professional Practice\n                            Committee","year":"2024","journal-title":"Diabetes Care"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"1839","DOI":"10.1007\/s00125-022-05721-6","article-title":"Precision nutrition in diabetes: When population-based dietary advice gets\n                    personal","volume":"65","author":"J Merino","year":"2022","journal-title":"Diabetologia"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1038\/s41588-025-02161-x","article-title":"Multi-omic and spatial analysis of mouse kidneys highlights sex-specific\n                    differences in gene regulation across the lifespan","volume":"57","author":"S Chen","year":"2025","journal-title":"Nat Genet"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1159\/000101471","article-title":"Podocyte injury and glomerulosclerosis in hyperhomocysteinemic rats","volume":"27","author":"F Yi","year":"2007","journal-title":"Am J Nephrol"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"107834","DOI":"10.1016\/j.jdiacomp.2020.107834","article-title":"Homocysteine and diabetes: Role in macrovascular and microvascular\n                    complications","volume":"35","author":"E Muzurovi\u0107","year":"2021","journal-title":"J Diabetes Complications"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/S1357-2725(99)00140-5","article-title":"DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A\n                    and 2A genes is tissue-specific","volume":"32","author":"L Torre","year":"2000","journal-title":"Int J Biochem Cell Biol"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"1664","DOI":"10.1016\/j.chembiol.2022.11.009","article-title":"Metabolic modulation of transcription: The role of one-carbon metabolism","volume":"29","author":"J G Lin","year":"2022","journal-title":"Cell Chem Biol"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1007\/s12038-011-9171-5","article-title":"Corticoadrenal activity in rat regulates betaine-homocysteine\n                    S-methyltransferase expression with opposite effects in liver and kidney","volume":"37","author":"O Fridman","year":"2012","journal-title":"J Biosci"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.2337\/dc22-S005","article-title":"5. Facilitating behavior change and well-being to improve health outcomes:\n                    Standards of medical care in diabetes\u20142022","volume":"45","author":"Committee ADAPP","year":"2022","journal-title":"Diabetes Care"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"105602","DOI":"10.1016\/j.phrs.2021.105602","article-title":"Role of dietary modifications in the management of type 2 diabetic\n                    complications","volume":"168","author":"M J Oza","year":"2021","journal-title":"Pharmacol Res"}],"container-title":["Experimental and Clinical Endocrinology &amp; Diabetes"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2686-7562.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T23:34:27Z","timestamp":1764113667000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2686-7562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":35,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,11,25]]},"published-print":{"date-parts":[[2025,11]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2686-7562","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0947-7349","1439-3646"],"issn-type":[{"value":"0947-7349","type":"print"},{"value":"1439-3646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,29]]}}}