{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T23:21:24Z","timestamp":1778800884757,"version":"3.51.4"},"reference-count":20,"publisher":"Georg Thieme Verlag KG","issue":"03","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100023015","name":"Advanced Research Projects Agency for Health","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100023015","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Knee Surg"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This preclinical ex vivo study was conducted to evaluate the effects of submersion in saline or the Missouri Osteochondral Preservation System (MOPS\u00ae) solution during reaming on viable chondrocyte density (VCD) of osteochondral allografts (OCAs). Distal femoral OCAs preserved with MOPS were reamed to create cylindrical \u201cplug\u201d grafts using one of three techniques: Submersion in MOPS (SG\u2013MOPS), submersion in saline (SG\u2013Saline), or reamer saturated with MOPS without OCA submersion (SR\u2013MOPS). All plug reaming was performed using standardized instrumentation and technique to harvest 18-mm-diameter plugs. Pre-reaming cartilage samples were collected to confirm baseline VCD. Post-reaming plugs were bisected and stained for live\/dead analysis using fluorescent microscopy. VCD was quantified via image analysis, and %Day-0 VCD was calculated. Group comparisons were made using one-way analysis of variance (ANOVA; \u03b1\u2009=\u20090.05). A total of 21 plugs from 9 donors were analyzed: SG\u2013MOPS (n\u2009=\u20098), SG\u2013Saline (n\u2009=\u20096), SR\u2013MOPS (n\u2009=\u20097). Mean %Day-0 VCD was highest in SG\u2013MOPS (92.6\u2009\u00b1\u20097.8%), followed by SG\u2013Saline (83.3\u2009\u00b1\u200910.2%), and SR\u2013MOPS (80.2\u2009\u00b1\u20099.1%), though differences were not statistically significant (p\u2009=\u20090.68). A higher proportion of SG\u2013MOPS plugs (7\/8, 88%) exceeded the minimum essential VCD threshold (70%) compared with SG\u2013Saline (4\/6, 67%) and SR\u2013MOPS (3\/7, 43%). Submerging distal femur OCAs in MOPS during 18-mm-diameter femoral condyle plug reaming had clinically meaningful beneficial effects on viable donor chondrocyte density when compared with saline-submerged or non-submerged grafts. Based on the use of this submerged reaming technique that is standardized, repeatable, readily available, cost-effective, and safe, this methodology can be considered \u201cbest practice\u201d for OCA plug reaming protocols, motivating implementation of this evidence-based shift in practice at our institution.<\/jats:p>","DOI":"10.1055\/a-2695-2258","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T22:53:59Z","timestamp":1757026439000},"page":"166-170","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Osteochondral Allograft Reaming Protocols on Donor Chondrocyte Viability Prior to Transplantation"],"prefix":"10.1055","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8627-1500","authenticated-orcid":false,"given":"Kylee","family":"Rucinski","sequence":"additional","affiliation":[{"name":"Mizzou Joint Preservation Center and Thompson Laboratory for Regenerative Orthopaedics, Missouri Orthopaedic Institute, University of Missouri, Columbia, Missouri, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6403-3969","authenticated-orcid":false,"given":"Aaron M.","family":"Stoker","sequence":"additional","affiliation":[{"name":"Mizzou Joint Preservation Center and Thompson Laboratory for Regenerative Orthopaedics, Missouri Orthopaedic Institute, University of Missouri, Columbia, Missouri, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8467-6494","authenticated-orcid":false,"given":"James P.","family":"Stannard","sequence":"additional","affiliation":[{"name":"Mizzou Joint Preservation Center and Thompson Laboratory for Regenerative Orthopaedics, Missouri Orthopaedic Institute, University of Missouri, Columbia, Missouri, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4859-224X","authenticated-orcid":false,"given":"Clayton W.","family":"Nuelle","sequence":"additional","affiliation":[{"name":"Mizzou Joint Preservation Center and Thompson Laboratory for Regenerative Orthopaedics, Missouri Orthopaedic Institute, University of Missouri, Columbia, Missouri, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob S.","family":"Kramer","sequence":"additional","affiliation":[{"name":"Mizzou Joint Preservation Center and Thompson Laboratory for Regenerative Orthopaedics, Missouri Orthopaedic Institute, University of Missouri, Columbia, Missouri, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corder E.","family":"Lehenbauer","sequence":"additional","affiliation":[{"name":"Mizzou Joint Preservation Center and Thompson Laboratory for Regenerative Orthopaedics, Missouri Orthopaedic Institute, University of Missouri, Columbia, Missouri, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0862-995X","authenticated-orcid":false,"given":"James L.","family":"Cook","sequence":"additional","affiliation":[{"name":"Mizzou Joint Preservation Center and Thompson Laboratory for Regenerative Orthopaedics, Missouri Orthopaedic Institute, University of Missouri, Columbia, Missouri, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"issue":"14","key":"ref1","doi-asserted-by":"crossref","first-page":"3541","DOI":"10.1177\/0363546517732531","article-title":"Clinical outcomes and failure rates of osteochondral allograft transplantation in the knee: A systematic review","volume":"46","author":"F Familiari","year":"2018","journal-title":"Am J Sports Med"},{"issue":"10","key":"ref2","doi-asserted-by":"crossref","first-page":"2160","DOI":"10.1016\/j.arthro.2016.04.020","article-title":"Long-term outcomes after osteochondral allograft: A systematic review at long-term follow-up of 12.3 years","volume":"32","author":"A T Assenmacher","year":"2016","journal-title":"Arthroscopy"},{"issue":"05","key":"ref3","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1055\/a-2506-2675","article-title":"A bedside-to-bench-to-bedside journey to advance osteochondral allograft transplantation towards biologic joint restoration","volume":"38","author":"J L Cook","year":"2025","journal-title":"J Knee Surg"},{"issue":"03","key":"ref4","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1177\/03635465221144003","article-title":"Initial outcomes after unicompartmental tibiofemoral bipolar osteochondral and meniscal allograft transplantation in the knee using MOPS-preserved fresh (viable) tissues","volume":"51","author":"J L Cook","year":"2023","journal-title":"Am J Sports Med"},{"issue":"06","key":"ref5","first-page":"23","article-title":"Assessment of outcomes after multisurface osteochondral allograft transplantations in the knee","volume":"10","author":"J L Cook","year":"2022","journal-title":"Orthop J Sports Med"},{"issue":"03","key":"ref6","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1177\/0363546517744202","article-title":"Outcomes of osteochondral allograft transplantation with and without concomitant meniscus allograft transplantation: A comparative matched group analysis","volume":"46","author":"R M Frank","year":"2018","journal-title":"Am J Sports Med"},{"issue":"11","key":"ref7","doi-asserted-by":"crossref","first-page":"2325967119884291","DOI":"10.1177\/2325967119884291","article-title":"Effects of compliance with procedure-specific postoperative rehabilitation protocols on initial outcomes after osteochondral and meniscal allograft transplantation in the knee","volume":"7","author":"K Rucinski","year":"2019","journal-title":"Orthop J Sports Med"},{"issue":"05","key":"ref8","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1177\/0363546516629434","article-title":"Importance of donor chondrocyte viability for osteochondral allografts","volume":"44","author":"J L Cook","year":"2016","journal-title":"Am J Sports Med"},{"issue":"03","key":"ref9","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/j.arthro.2022.10.007","article-title":"The Missouri Osteochondral Preservation System is associated with better short-term outcomes than standard preservation methods when performing osteochondral allograft transplantation using shell grafts for patellofemoral lesions","volume":"39","author":"A F Buyuk","year":"2023","journal-title":"Arthroscopy"},{"issue":"08","key":"ref10","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1055\/s-0037-1608947","article-title":"Chondrocyte viability at time of transplantation for osteochondral allografts preserved by the Missouri Osteochondral Preservation System versus standard tissue bank protocol","volume":"31","author":"A M Stoker","year":"2018","journal-title":"J Knee Surg"},{"issue":"07","key":"ref11","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1002\/jor.24290","article-title":"Metabolic responses of osteochondral allografts to re-warming","volume":"37","author":"A M Stoker","year":"2019","journal-title":"J Orthop Res"},{"issue":"01","key":"ref12","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1177\/0363546517727516","article-title":"Validation of the Missouri Osteochondral Allograft Preservation System for the maintenance of osteochondral allograft quality during prolonged storage","volume":"46","author":"A M Stoker","year":"2018","journal-title":"Am J Sports Med"},{"issue":"12","key":"ref13","doi-asserted-by":"crossref","first-page":"2797","DOI":"10.1177\/0363546517715725","article-title":"Effects of autogenous bone marrow aspirate concentrate on radiographic integration of femoral condylar osteochondral allografts","volume":"45","author":"L O Oladeji","year":"2017","journal-title":"Am J Sports Med"},{"issue":"01","key":"ref14","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1055\/s-0038-1636834","article-title":"Comparison of techniques for preimplantation treatment of osteochondral allograft bone","volume":"32","author":"C A Baumann","year":"2019","journal-title":"J Knee Surg"},{"issue":"01","key":"ref15","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1055\/s-0038-1636925","article-title":"Large osteochondral allografts of the knee: Surgical technique and indications","volume":"6","author":"G Pisanu","year":"2018","journal-title":"Joints"},{"issue":"06","key":"ref16","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1177\/0363546520907101","article-title":"Prospective assessment of outcomes after primary unipolar, multisurface, and bipolar osteochondral allograft transplantations in the knee: A comparison of 2 preservation methods","volume":"48","author":"J P Stannard","year":"2020","journal-title":"Am J Sports Med"},{"issue":"06","key":"ref17","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1177\/03635465211016832","article-title":"Fresh osteochondral and chondral allograft preservation and storage media: A systematic review of the literature","volume":"50","author":"S M Tabbaa","year":"2022","journal-title":"Am J Sports Med"},{"issue":"09","key":"ref18","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1177\/0363546508316765","article-title":"Effect of osteochondral graft insertion forces on chondrocyte viability","volume":"36","author":"S Patil","year":"2008","journal-title":"Am J Sports Med"},{"issue":"11","key":"ref19","doi-asserted-by":"crossref","first-page":"2874","DOI":"10.1177\/03635465241268969","article-title":"Osteochondral allograft reaming significantly affects chondrocyte viability","volume":"52","author":"T J Elias","year":"2024","journal-title":"Am J Sports Med"},{"issue":"01","key":"ref20","first-page":"100105","article-title":"Osteochondral techniques: Where are we now?","volume":"3","author":"G Medina","year":"2023","journal-title":"J Cartil Jt Preserv"}],"container-title":["The Journal of Knee Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2695-2258.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T23:36:56Z","timestamp":1770248216000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2695-2258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,4]]},"references-count":20,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2026,2,4]]},"published-print":{"date-parts":[[2026,2]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2695-2258","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["1538-8506","1938-2480"],"issn-type":[{"value":"1538-8506","type":"print"},{"value":"1938-2480","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,9,4]]},"published":{"date-parts":[[2025,9,4]]}}}