{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T11:00:04Z","timestamp":1768647604787,"version":"3.49.0"},"reference-count":40,"publisher":"Georg Thieme Verlag KG","issue":"01","funder":[{"DOI":"10.13039\/501100009975","name":"Charotar University of Science and\n                        Technology","doi-asserted-by":"crossref","award":["CHARUSAT SEED RESEARCH GRANT (RP4\/21)"],"award-info":[{"award-number":["CHARUSAT SEED RESEARCH GRANT (RP4\/21)"]}],"id":[{"id":"10.13039\/501100009975","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Res (Stuttg)"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Colorectal cancer accounts for about 10% of all cancer diagnoses and is the\n                    second leading cause of cancer-related death. Until the cancer has progressed to\n                    an advanced stage, colorectal cancer typically shows no symptoms. The\n                    medications currently utilized to treat colorectal cancer have serious\n                    drawbacks. The aim of the study was to evaluate the ability of newly developed\n                    synthetic matrix metalloprotease-2 inhibitors to prevent metastases. This study\n                    used computational models to evaluate pharmacokinetic characteristics and\n                    ligand-binding affinities for preliminary screening. Later, we used the human\n                    colorectal cancer cell line SW620 in an in ovo approach to assess its\n                    anti-migratory properties. The chorioallantoic membrane was used to transplant\n                    tumor cells. In order to assess migrastatic activity in a preclinical model\n                    using an in ovo technique, we measured changes in the chick embryo's total\n                    body weight, colon length and body weight, complete blood count and\n                    histopathology of the colon, liver and stomach. In addition, the histology of\n                    the stomach, liver, colon, and chorioallantoic membrane was taken into account\n                    while assessing the activity. We discovered that compound N5\n                    ({4-[(3\u2032-amino[1,1\u2032-biphenyl]-4-carbonyl) amino] phenyl~acetic acid) had the\n                    best migrastatic action by blocking matrix metalloprotease-2. The new compound\n                    showed potential as a matrix metalloprotease-2 inhibitor compared to\n                    doxycycline. A more robust platform was needed for further investigation and\n                    progress of novel compound towards clinical setting.<\/jats:p>","DOI":"10.1055\/a-2724-6878","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:07:16Z","timestamp":1764374836000},"page":"5-13","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Migrastatic Properties of Selective MMP-2\n                    Inhibitors in Colorectal Cancer: An In Ovo Approach"],"prefix":"10.1055","volume":"76","author":[{"given":"Nikunj","family":"Parekh","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mansi","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Praysha","family":"Patel","sequence":"additional","affiliation":[{"name":"University of Windsor, Windsor, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Disha","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirtan","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishna","family":"Thakkar","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aarti","family":"Parmar","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umang","family":"Shah","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahil","family":"Panjvani","sequence":"additional","affiliation":[{"name":"Department of Histopathology, Vadodara Clinical Laboratory, Vadodara,\n                    India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samir","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alkeshkumar Kiritbhai","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar\n                    University of Science and Technology, Changa, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2025,11,28]]},"reference":[{"issue":"01","key":"ref1","first-page":"1","article-title":"Colorectal cancer: global and Brazilian perspectives, prevention, and the impact\n                    of the Blue March Campaign","volume":"62","author":"M Averbach","year":"2025","journal-title":"Arq Gastroenterol"},{"issue":"04","key":"ref2","first-page":"372","article-title":"Clinical management of metastatic colorectal cancer in the era of precision\n                    medicine","volume":"72","author":"F Ciardiello","year":"2022","journal-title":"CA Cancer J Clin"},{"issue":"05","key":"ref3","doi-asserted-by":"crossref","first-page":"941","DOI":"10.3390\/cancers16050941","article-title":"Contemporary surgical management of colorectal liver metastases","volume":"16","author":"P Chandra","year":"2024","journal-title":"Cancers"},{"issue":"10","key":"ref4","doi-asserted-by":"crossref","first-page":"761","DOI":"10.5306\/wjco.v11.i10.761","article-title":"Colorectal liver metastases: current management and future perspectives","volume":"11","author":"J Martin","year":"2020","journal-title":"World J Clin Oncol"},{"issue":"01","key":"ref5","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.annonc.2022.10.003","article-title":"Metastatic colorectal cancer: ESMO clinical practice guideline for diagnosis,\n                    treatment and follow-up","volume":"34","author":"A Cervantes","year":"2023","journal-title":"Ann Oncol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"7407190","DOI":"10.1155\/2019\/7407190","article-title":"Mechanisms of metastasis in colorectal cancer and metastatic organotropism:\n                    hematogenous versus peritoneal spread","volume":"2019","author":"E Pretzsch","year":"2019","journal-title":"J Oncol"},{"issue":"1\/2","key":"ref7","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1615\/CritRevOncog.v18.i1-2.40","article-title":"On the origin of cancer metastasis","volume":"18","author":"T N Seyfried","year":"2013","journal-title":"Crit Rev Oncog"},{"key":"ref8","first-page":"186","volume-title":"Notch signaling pathway and cancer metastasis","author":"Y Y Hu","year":"2012"},{"issue":"05","key":"ref9","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.apsb.2015.07.005","article-title":"Cancer metastases: challenges and opportunities","volume":"5","author":"X Guan","year":"2015","journal-title":"Acta Pharm Sin B"},{"issue":"01","key":"ref10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12935-022-02801-w","article-title":"Current methods for studying metastatic potential of tumor cells","volume":"22","author":"P Bouchalova","year":"2022","journal-title":"Cancer Cell Int"},{"issue":"02","key":"ref11","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1538\/expanim.14-0059","article-title":"Chick embryo chorioallantoic membrane (CAM): an alternative predictive model in\n                    acute toxicological studies for anticancer drugs","volume":"64","author":"C S Kue","year":"2015","journal-title":"Exp Anim"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"791081","DOI":"10.3389\/fimmu.2021.791081","article-title":"The chicken embryo model: a novel and relevant model for immune based\n                    studies","volume":"12","author":"P Garcia","year":"2021","journal-title":"Front Immunol"},{"issue":"01","key":"ref13","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1038\/s41392-023-01419-2","article-title":"Patient derived xenograft models in cancer therapy: technologies and\n                    applications","volume":"8","author":"Y Liu","year":"2023","journal-title":"Signal Transduct Target Ther"},{"issue":"03","key":"ref14","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/B978-0-12-811710-1.00031-8","article-title":"Chick chorioallantoic membrane assay: a 3D animal model for cancer invasion and\n                    metastasis","volume":"31","author":"N A Lokman","year":"2020","journal-title":"Anim Biotechnol"},{"issue":"23","key":"ref15","doi-asserted-by":"crossref","first-page":"4139","DOI":"10.1002\/cncr.32163","article-title":"Targeted therapy for colorectal cancer metastases: a review of current methods\n                    of molecularly targeted therapy and the use of tumor biomarkers in the treatment\n                    of metastatic colorectal cancer","volume":"125","author":"S Piawah","year":"2019","journal-title":"Cancer"},{"issue":"04","key":"ref16","doi-asserted-by":"crossref","first-page":"151075","DOI":"10.1016\/j.ejcb.2020.151075","article-title":"Microtubule-targeting agents and their impact on cancer treatment","volume":"99","author":"V \u010cerm\u00e1k","year":"2020","journal-title":"Eur J Cell Biol"},{"issue":"06","key":"ref17","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.trecan.2017.04.008","article-title":"Migrastatics\u2014antimetastatic and anti-invasion drugs: promises and\n                    challenges","volume":"3","author":"A Gandalovi\u010dov\u00e1","year":"2017","journal-title":"Trends Cancer"},{"issue":"01","key":"ref18","first-page":"233","article-title":"Cancer statistics, 2023","volume":"73","author":"R L Siegel","year":"2023","journal-title":"CA Cancer J Clin"},{"issue":"01","key":"ref19","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3390\/biom15010035","article-title":"Targeting invasion: the role of MMP-2 and MMP-9 inhibition in colorectal cancer\n                    therapy","volume":"15","author":"A Shoari","year":"2024","journal-title":"Biomolecules"},{"issue":"01","key":"ref20","doi-asserted-by":"crossref","first-page":"366","DOI":"10.3390\/cancers6010366","article-title":"The role of matrix metalloproteinases in colorectal cancer","volume":"6","author":"A H Said","year":"2014","journal-title":"Cancers"},{"issue":"06","key":"ref21","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1158\/1535-7163.MCT-17-0646","article-title":"Matrix metalloproteinase inhibitors in cancer therapy: turning past failures\n                    into future successes","volume":"17","author":"A Winer","year":"2018","journal-title":"Mol Cancer Ther"},{"key":"ref22","first-page":"3249766","article-title":"Matrix metalloproteinases in chemoresistance: regulatory roles, molecular\n                    interactions, and potential inhibitors","volume":"2022","author":"B X Tune","year":"2022","journal-title":"J Oncol"},{"issue":"01","key":"ref23","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3390\/biom15010035","article-title":"Targeting invasion: the role of MMP-2 and MMP-9 Inhibition in colorectal cancer\n                    therapy","volume":"15","author":"A Shoari","year":"2024","journal-title":"Biomolecules"},{"issue":"01","key":"ref24","doi-asserted-by":"crossref","first-page":"7505","DOI":"10.1038\/s41598-020-64290-z","article-title":"Tumor grafts grown on the chicken chorioallantoic membrane are distinctively\n                    characterized by MRI under functional gas challenge","volume":"10","author":"C F Waschkies","year":"2020","journal-title":"Sci Rep"},{"issue":"03","key":"ref25","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.oraloncology.2009.11.013","article-title":"Anti-invasion and anti-tumor growth effect of doxycycline treatment for human\n                    oral squamous cell carcinoma: in vitro and in vivo studies","volume":"46","author":"L C Shen","year":"2010","journal-title":"Oral Oncol"},{"issue":"03","key":"ref26","doi-asserted-by":"crossref","first-page":"e70012","DOI":"10.1111\/fcp.70012","article-title":"Matrix metalloproteinase 9 implication during colorectal carcinogenesis. Effect\n                    of doxycycline","volume":"39","author":"A Bounaama","year":"2025","journal-title":"Fundam Clin Pharmacol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"S560","DOI":"10.4103\/jcrt.jcrt_1299_22","article-title":"Investigating the resistance mechanism of 5-fluorouracil in colorectal cancer\n                    based on surface markers of cancer stemness and cytokine level: A pre-clinical\n                    study","volume":"19","author":"S G Patel","year":"2023","journal-title":"J Cancer Res Ther"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"176979","DOI":"10.1016\/j.ejphar.2024.176979","article-title":"Resistance to 5-fluorouracil: The molecular mechanisms of development in colon\n                    cancer cells","volume":"983","author":"N Radenkovi\u0107","year":"2024","journal-title":"Eur J 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Cancer"},{"issue":"01","key":"ref32","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1186\/s12885-020-07429-5","article-title":"Neutrophil-to-lymphocyte ratio is a prognostic factor for colon cancer: a\n                    propensity score analysis","volume":"20","author":"J Mazaki","year":"2020","journal-title":"BMC cancer"},{"issue":"01","key":"ref33","doi-asserted-by":"crossref","first-page":"3360","DOI":"10.1038\/s41598-020-60255-4","article-title":"The prognostic implications of tumor infiltrating lymphocytes in colorectal\n                    cancer: a systematic review and meta-analysis","volume":"10","author":"G E Idos","year":"2020","journal-title":"Sci Rep"},{"issue":"20","key":"ref34","doi-asserted-by":"crossref","first-page":"6412","DOI":"10.1158\/1078-0432.CCR-09-1438","article-title":"Lymphocytic reaction to colorectal cancer is associated with longer survival,\n                    independent of lymph node count, microsatellite instability, and CpG island\n                    methylator phenotype","volume":"15","author":"S Ogino","year":"2009","journal-title":"Clin Cancer Res"},{"issue":"02","key":"ref35","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1177\/1724600818817320","article-title":"Clinicopathological significance and prognostic role of tumor infiltrating\n                    lymphocytes in colorectal cancer","volume":"34","author":"Y S Ko","year":"2019","journal-title":"Int J Biol Markers"},{"issue":"01","key":"ref36","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s13550-024-01151-0","article-title":"An in vivo tumour organoid model based on the chick embryonic chorioallantoic\n                    membrane mimics key characteristics of the patient tissue: a proof-of-concept\n                    study","volume":"14","author":"K Ben\u010durov\u00e1","year":"2024","journal-title":"EJNMMI Res"},{"issue":"01","key":"ref37","doi-asserted-by":"crossref","first-page":"10472","DOI":"10.1038\/s41598-020-67492-7","article-title":"CAM-Delam: an in vivo approach to visualize and quantify the delamination and\n                    invasion capacity of human cancer cells","volume":"10","author":"T K Palaniappan","year":"2020","journal-title":"Sci Rep"},{"issue":"01","key":"ref38","first-page":"140","article-title":"A novel in ovo model to study cancer metastasis using chicken embryos and GFP\n                    expressing cancer cells","volume":"20","author":"R Augustine","year":"2020","journal-title":"Bosn J Basic Med Sci"},{"issue":"01","key":"ref39","doi-asserted-by":"crossref","first-page":"7505","DOI":"10.1038\/s41598-020-64290-z","article-title":"Tumor grafts grown on the chicken chorioallantoic membrane are distinctively\n                    characterized by MRI under functional gas challenge","volume":"10","author":"C F Waschkies","year":"2020","journal-title":"Sci Rep"},{"issue":"05","key":"ref40","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.52711\/0974-360X.2024.00340","article-title":"Evaluating the Immunomodulatory and Detoxifying role of Tinospora cordifolia and\n                    Solanum nigrum in the treatment of Colorectal Cancer","volume":"17","author":"A Patel","year":"2024","journal-title":"Res J Pharm Technol"}],"container-title":["Drug Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2724-6878.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T23:38:52Z","timestamp":1768606732000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2724-6878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,28]]},"references-count":40,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2026,1,16]]},"published-print":{"date-parts":[[2026,1]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2724-6878","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["2194-9379","2194-9387"],"issn-type":[{"value":"2194-9379","type":"print"},{"value":"2194-9387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,28]]}}}