{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T23:15:49Z","timestamp":1778627749812,"version":"3.51.4"},"reference-count":33,"publisher":"Georg Thieme Verlag KG","issue":"06","funder":[{"name":"Kooperation Phytopharmaka GbR"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>HMPs derived from Hypericum perforatum, herba (St.\u00a0John\u02bcs wort) play an important role in treating mild to moderate depressive episodes and mental exhaustion, for example. The genotoxic safety of these HMPs has already been assessed and reviewed in recent years. However, further data on the genotoxic potential of Hypericum perforatum, herba preparations would allow the evaluation of the therapeutic safety of the herbal drug and preparations thereof in accordance with the genotoxicity guideline of the HMPC of the EMA. Accordingly, their genotoxic potential was tested using the \u201cbracketing and matrixing\u201d concept, whereby extracts are tested at and between the extremes of their extraction solvents (ranging in strength from polar to non-polar) to cover the entire spectrum of the drug\u02bcs constituents. In the present study, five dry extracts of Hypericum perforatum, herba, representing the full range of potential extraction solvent polarities (water, ethanol\n          50% (v\/v), methanol 80% (v\/v), ethanol 80% (v\/v), and n-heptane) were tested using the Ames test, according to the OECD and HMPC guidelines. No mutagenic effects were observed for any of the five test strains in two independent experiments (plate incorporation test and pre-incubation test) each carried out with and without metabolic activation, even at the highest concentrations (5000\u2009\u00b5g\/plate), in accordance with the OECD guideline No.\u2009471.<\/jats:p>","DOI":"10.1055\/a-2727-1619","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T23:58:44Z","timestamp":1762905524000},"page":"639-646","source":"Crossref","is-referenced-by-count":0,"title":["Genotoxicity Assessment of Hypericum perforatum Dry Extracts in the Ames Test Based on the \u201cBracketing and Matrixing\u201d Concept"],"prefix":"10.1055","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2050-724X","authenticated-orcid":false,"given":"Jens","family":"Flade","sequence":"additional","affiliation":[{"name":"Rudolf-Boehm-Institut f\u00fcr Pharmakologie und Toxikologie, Postgraduate Study of Toxicology and Environmental Protection, Leipzig University, Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3334-5833","authenticated-orcid":false,"given":"Olaf","family":"Kelber","sequence":"additional","affiliation":[{"name":"Phyto & Biotics Technology Platform, Bayer Consumer Health, Steigerwald Arzneimittelwerk GmbH, Darmstadt, Germany"},{"name":"Kooperation Phytopharmaka GbR, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Nieber","sequence":"additional","affiliation":[{"name":"Kooperation Phytopharmaka GbR, Bonn, Germany"},{"name":"Institute for Pharmacy, Leipzig University, Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bj\u00f6rn","family":"Feistel","sequence":"additional","affiliation":[{"name":"Kooperation Phytopharmaka GbR, Bonn, Germany"},{"name":"Finzelberg GmbH & Co. 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