{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:23:33Z","timestamp":1783466613861,"version":"3.55.0"},"reference-count":23,"publisher":"Georg Thieme Verlag KG","issue":"02","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":22,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This study has been supported by the Diasorin Italia\n                        S.p.A."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Graves\u2019 disease is an autoimmune disease caused by autoantibodies to the\n                    thyroid-stimulating hormone receptor, which usually leads to hyperthyroidism in\n                    these patients. The aim of the present study was to evaluate a new automated\n                    bridge chemiluminescence thyroid-stimulating hormone receptor autoantibody assay\n                    for the diagnosis and in the follow-up of Graves\u2019 disease patients and to\n                    compare this assay with another established competition assay. Altogether, 132\n                    Graves\u2019 disease, 39 autoimmune thyroiditis, 28 non-autoimmune nodular goiter and\n                    27 thyroid cancer patients were included in this study. Receiver-operating\n                    characteristic plot analysis based on above mentioned samples revealed an area\n                    under the curve of 0.9994 (95% confidence interval: 0.9980\u20131.001), indicating\n                    high sensitivity and specificity of the assay. The optimal sensitivity (99.22%;\n                    95% confidence interval: 95.7\u201399.8%) and specificity (98.98%; 95% confidence\n                    interval: 94.4%\u201399.8%) were seen at a cut-off level of 0.550 IU\/L. The\n                    corresponding positive predictive value was 99.22%, whereas the negative\n                    predictive value was estimated to be 98.98%. A detailed comparison of\n                    the two assay systems used revealed a slightly different thyroid-stimulating\n                    hormone receptor autoantibody distribution with the new bridge assay detecting\n                    more thyroid-stimulating hormone receptor autoantibody-positive follow-up\n                    patients with active Graves\u2019 disease compared with the competition assay. The\n                    measurement of thyroid-stimulating hormone receptor autoantibodies revealed a\n                    steady decline over the time of follow-up. In summary, our results demonstrate\n                    that the new automated bridge assay for detecting thyroid-stimulating hormone\n                    receptor autoantibodies has high sensitivity and specificity for diagnosing\n                    Graves\u2019 disease and to discriminate from other thyroid diseases,\n                    respectively.<\/jats:p>","DOI":"10.1055\/a-2797-6441","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T23:50:52Z","timestamp":1771890652000},"page":"56-52","source":"Crossref","is-referenced-by-count":0,"title":["Clinical Evaluation of a New Automated Bridge Assay for the Detection\n                    of Thyroid-Stimulating Hormone Receptor Autoantibodies"],"prefix":"10.1055","volume":"58","author":[{"given":"Patricia","family":"Schott-Ohly","sequence":"additional","affiliation":[{"name":"Division for Specific Endocrinology, University\n                    Hospital Duesseldorf Centre for Health and Society, Duesseldorf,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samer","family":"Albeiruty","sequence":"additional","affiliation":[{"name":"Division for Specific Endocrinology, University\n                    Hospital Duesseldorf Centre for Health and Society, Duesseldorf,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mareike","family":"Muchalla","sequence":"additional","affiliation":[{"name":"Division for Specific Endocrinology, Medical Faculty, University\n                    Hospital Duesseldorf, Duesseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Doetter","sequence":"additional","affiliation":[{"name":"Division for Specific Endocrinology, University\n                    Hospital Duesseldorf Centre for Health and Society, Duesseldorf,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jann","family":"Hommen","sequence":"additional","affiliation":[{"name":"Division for Specific Endocrinology, University\n                    Hospital Duesseldorf Centre for Health and Society, Duesseldorf,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Eberwein","sequence":"additional","affiliation":[{"name":"Division for Specific Endocrinology, University\n                    Hospital Duesseldorf Centre for Health and Society, Duesseldorf,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiana","family":"Vogkou","sequence":"additional","affiliation":[{"name":"Division for Specific Endocrinology, University\n                    Hospital Duesseldorf Centre for Health and Society, Duesseldorf,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derik","family":"Hermsen","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory\n                    Medicine, University Hospital Duesseldorf Centre for Health and Society,\n                    Duesseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joerg","family":"Timm","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Duesseldorf\n                    Centre for Health and Society, Duesseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"MacKenzie","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hospital Hygiene,\n                    University Hospital Duesseldorf Centre for Health and Society, Duesseldorf,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederik","family":"Giesel","sequence":"additional","affiliation":[{"name":"Clinic for Nuclear Medicine, University Hospital Duesseldorf,\n                    Duesseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Schott","sequence":"additional","affiliation":[{"name":"Division for Specific Endocrinology, University\n                    Hospital Duesseldorf Centre for Health and Society, Duesseldorf,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1038\/nrendo.2013.193","article-title":"Diagnosis and management of graves disease: a global overview","volume":"9","author":"L Bartalena","year":"2013","journal-title":"Nat Rev Endocrinol"},{"issue":"05","key":"ref2","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/nrendo.2018.18","article-title":"Global epidemiology of hyperthyroidism and hypothyroidism","volume":"14","author":"P N Taylor","year":"2018","journal-title":"Nat Rev Endocrinol"},{"issue":"04","key":"ref3","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/S2213-8587(23)00005-0","article-title":"Hyperthyroidism: aetiology, pathogenesis, diagnosis, management, complications,\n                    and prognosis","volume":"11","author":"W M Wiersinga","year":"2023","journal-title":"Lancet Diabetes Endocrinol"},{"issue":"01","key":"ref4","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1038\/s41572-020-0184-y","article-title":"Graves\u2019 disease","volume":"6","author":"T F Davies","year":"2020","journal-title":"Nat Rev Dis Primers"},{"issue":"09","key":"ref5","doi-asserted-by":"crossref","first-page":"4199","DOI":"10.4049\/jimmunol.1500183","article-title":"Graves\u2019 disease TSHR-stimulating antibodies (TSAbs) induce the activation of\n                    immature thymocytes: a clue to the riddle of TSAbs generation?","volume":"194","author":"M Gimenez-Barcons","year":"2015","journal-title":"J Immunol"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1055\/s-0035-1559633","article-title":"Graves\u2019 disease mechanisms: The role of stimulating, blocking, and cleavage\n                    region TSH receptor antibodies","volume":"47","author":"S A Morshed","year":"2015","journal-title":"Horm Metab Res"},{"issue":"06","key":"ref7","doi-asserted-by":"crossref","first-page":"R241","DOI":"10.1530\/EJE-14-0047","article-title":"Mechanisms in endocrinology: autoimmune thyroid disease: old and new\n                    players","volume":"170","author":"G Effraimidis","year":"2014","journal-title":"Eur J Endocrinol"},{"issue":"07","key":"ref8","doi-asserted-by":"crossref","first-page":"e0270890","DOI":"10.1371\/journal.pone.0270890","article-title":"Anti-TSH receptor antibodies (TRAb): Comparison of two third generation\n                    automated immunoassays broadly used in clinical laboratories and results\n                    interpretation","volume":"17","author":"J M Lopez Ortega","year":"2022","journal-title":"PLoS One"},{"issue":"06","key":"ref9","doi-asserted-by":"crossref","first-page":"918","DOI":"10.3390\/cells12060918","article-title":"Molecular mechanisms in autoimmune thyroid disease","volume":"12","author":"H Vargas-Uricoechea","year":"2023","journal-title":"Cells"},{"issue":"04","key":"ref10","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1111\/j.1365-2265.1982.tb01607.x","article-title":"An improved radioreceptor assay for TSH receptor antibodies","volume":"17","author":"G 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Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2797-6441.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:01:39Z","timestamp":1783465299000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2797-6441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":23,"journal-issue":{"issue":"02","published-online":{"date-parts":[[2026,2,27]]},"published-print":{"date-parts":[[2026,2]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2797-6441","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0018-5043","1439-4286"],"issn-type":[{"value":"0018-5043","type":"print"},{"value":"1439-4286","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]}}}