{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T11:40:28Z","timestamp":1772624428849,"version":"3.50.1"},"reference-count":46,"publisher":"Georg Thieme Verlag KG","funder":[{"name":"Foundation for Scientific and Technical Project of Nansha, Guangzhou","award":["2022ZD005"],"award-info":[{"award-number":["2022ZD005"]}]},{"name":"Key-Area Research and Development Program of Guangdong Province","award":["2023B1111050008"],"award-info":[{"award-number":["2023B1111050008"]}]},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"crossref","award":["AB24010109"],"award-info":[{"award-number":["AB24010109"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"PI Project of Southern Marine Science and Engineering Guangdong Laboratory","award":["GML2021GD0807"],"award-info":[{"award-number":["GML2021GD0807"]}]},{"name":"Excellent Young Scholars of the Natural Science Foundation of Shandong Province","award":["2024HWYQ-035"],"award-info":[{"award-number":["2024HWYQ-035"]}]},{"name":"Young experts of Taishan Scholars","award":["tsqn202312088"],"award-info":[{"award-number":["tsqn202312088"]}]},{"name":"Taxonomist project, Chinese Academy of Sciences","award":["CAS-TAX-24-043"],"award-info":[{"award-number":["CAS-TAX-24-043"]}]},{"name":"Biological Resources Programme, Chinese Academy of Sciences"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"abstract":"<jats:p>Thrombosis is the key driver for ischemic events, including stroke, which is the leading cause of global mortality. However, current antithrombotic therapies carry substantial bleeding risks. Targeting protease-activated receptor-1 (PAR-1), a thrombin-activated receptor central to thrombus growth, represents a promising antithrombotic strategy for safer intervention.<\/jats:p>\n                  <jats:p>we mined the venom gland transcriptome of Conus spp., applied in silico Furin cleavage prediction, and synthesized and screened the anti-PAR-1 activity of a series of conotoxin-derived peptides.<\/jats:p>\n                  <jats:p>Cb-26 exhibited the strongest but reversible activity in inhibiting PAR-1-mediated platelet activation and aggregation in vitro. Crucially, Cb-26 inhibited platelet adhesion, neutrophil extracellular traps formation, and thrombus growth under shear conditions in whole blood from healthy donors. In murine models, Cb-26 significantly delayed carotid occlusion and reduced cerebral infarct size in photochemical-induced ischemic stroke, without affecting blood coagulation and bleeding time.<\/jats:p>\n                  <jats:p>These results suggest that Cb-26 is a selective and reversible PAR-1 antagonist and represents a promising antithrombotic candidate without apparent bleeding side effects.<\/jats:p>","DOI":"10.1055\/a-2815-5095","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:39:06Z","timestamp":1771547946000},"source":"Crossref","is-referenced-by-count":0,"title":["A Conotoxin-Derived Peptide Selectively Inhibits PAR-1 Mediated Thrombosis and Neutrophil Extracellular Traps Formation Without Affecting Hemostasis"],"prefix":"10.1055","author":[{"given":"Aili","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianyu","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haidong","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siwen","family":"Fang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaochi","family":"Xu","sequence":"additional","affiliation":[{"name":"Center for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyun","family":"Chen","sequence":"additional","affiliation":[{"name":"South China Sea Institute of Oceanology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanbin","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren","family":"Lai","sequence":"additional","affiliation":[{"name":"Center for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China"},{"name":"Key Laboratory of Bioactive Peptides of Yunnan Province, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, National Resource Center for Non-Human Primates, Kunming Primate Research Center, National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Sino-African Joint Research Center and Engineering Laboratory of Peptides, Kunming Institute of Zoology, Kunming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1161\/01.RES.0000267878.97021.ab","article-title":"Adhesion mechanisms in platelet function","volume":"100","author":"Z M Ruggeri","year":"2007","journal-title":"Circ Res"},{"issue":"06","key":"ref2","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1080\/10408363.2016.1200008","article-title":"Platelets are versatile cells: New discoveries in hemostasis, thrombosis, immune responses, tumor metastasis and beyond","volume":"53","author":"X R Xu","year":"2016","journal-title":"Crit Rev Clin Lab Sci"},{"issue":"05","key":"ref3","doi-asserted-by":"crossref","first-page":"700","DOI":"10.3324\/haematol.2008.003178","article-title":"Platelet receptors and signaling in the dynamics of thrombus formation","volume":"94","author":"J 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