{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T23:47:13Z","timestamp":1782258433721,"version":"3.54.5"},"reference-count":54,"publisher":"Georg Thieme Verlag KG","issue":"05","funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"crossref","award":["101120168\u2014INNOVATION"],"award-info":[{"award-number":["101120168\u2014INNOVATION"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Semin Thromb Hemost"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Red blood cells (RBCs) must be highly deformable to pass through capillaries narrower than their own diameter for delivering oxygen to the tissues. Ion channels are central regulators of RBC deformability and volume. Gain-of-function mutations in Piezo1, as seen in hereditary xerocytosis, cause RBC dehydration and have been associated with an increased risk of thromboembolism. Here we investigated how pharmacological RBC-Piezo1 activation affects RBC membrane potential, RBC ion content, RBC deformability, coagulation dynamics, and clot structure.<\/jats:p>\n                  <jats:p>Blood samples were collected from healthy donors. RBCs were isolated and incubated with Yoda1, a drug known to activate Piezo1. Membrane potential was measured using the Macey\u2013Bennekou\u2013Eg\u00e9e method. Intracellular Na+\/K+ content was determined by flame photometry. RBC deformability was measured by ektacytometry. Coagulation dynamics were investigated by rotational thromboelastometry, and clot structure was observed by electron microscopy.<\/jats:p>\n                  <jats:p>Yoda1 treatment led to RBC membrane hyperpolarization, reduced cell volume, a decrease in intracellular K+, an increase in Na+, and a reduction in RBC deformability. ROTEM analysis showed shorter clotting and lysis times in Yoda1-treated samples compared with samples treated with the vehicle (control condition), independently of RBC phosphatidylserine exposure. Electron microscopy revealed structural alterations in Yoda1-treated samples, with less compacted RBCs.<\/jats:p>\n                  <jats:p>Altogether, these findings indicate that Piezo1 activation disrupted RBC ion balance and membrane potential, leading to dehydration and decreased deformability. These alterations contributed to accelerated coagulation and compromised clot structure, suggesting a potential role of Piezo1 in modulating thrombotic risk in RBC-related disorders.<\/jats:p>","DOI":"10.1055\/a-2846-3643","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T23:14:51Z","timestamp":1775085291000},"page":"601-612","source":"Crossref","is-referenced-by-count":0,"title":["Red Blood Cell Piezo1 Activation Drives Faster Coagulation and Structural Alterations in Blood Clots through Red Blood Cell Deformability Impairment"],"prefix":"10.1055","volume":"52","author":[{"given":"Bita","family":"Asghariastanehei","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 EA7424, Team Vascular Biology and Red Blood Cell, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, Lyon, France"},{"name":"Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Martin","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 EA7424, Team Vascular Biology and Red Blood Cell, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, Lyon, France"},{"name":"Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elie","family":"Nader","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 EA7424, Team Vascular Biology and Red Blood Cell, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, Lyon, France"},{"name":"Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamdi","family":"Rezigue","sequence":"additional","affiliation":[{"name":"Service d'h\u00e9matologie-h\u00e9mostase, Hospices Civils de Lyon, Bron, France"},{"name":"EA 4609-H\u00e9mostase et Thrombose, Universit\u00e9 Claude Bernard Lyon I, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u00eblle de","family":"Wreede","sequence":"additional","affiliation":[{"name":"Service d'h\u00e9matologie-h\u00e9mostase, Hospices Civils de Lyon, Bron, France"},{"name":"EA 4609-H\u00e9mostase et Thrombose, Universit\u00e9 Claude Bernard Lyon I, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yesim","family":"Dargaud","sequence":"additional","affiliation":[{"name":"Service d'h\u00e9matologie-h\u00e9mostase, Hospices Civils de Lyon, Bron, France"},{"name":"EA 4609-H\u00e9mostase et Thrombose, Universit\u00e9 Claude Bernard Lyon I, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Nougier","sequence":"additional","affiliation":[{"name":"Service d'h\u00e9matologie-h\u00e9mostase, Hospices Civils de Lyon, Bron, France"},{"name":"EA 4609-H\u00e9mostase et Thrombose, Universit\u00e9 Claude Bernard Lyon I, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Joly","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 EA7424, Team Vascular Biology and Red Blood Cell, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, Lyon, France"},{"name":"Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, France"},{"name":"Laboratoire de Biochimie et de Biologie Mol\u00e9culaire, UF de Biochimie des Pathologies E\u00ecrythrocytaires, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lica","family":"Ni\u00f1o\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, France"},{"name":"Station Biologique de Roscoff, Sorbonne Universit\u00e9, CNRS, UMR8227 LBI2M, Roscoff, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Eg\u00e9e","sequence":"additional","affiliation":[{"name":"Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, France"},{"name":"Station Biologique de Roscoff, Sorbonne Universit\u00e9, CNRS, UMR8227 LBI2M, Roscoff, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Kaestner","sequence":"additional","affiliation":[{"name":"Theoretical Medicine and Biosciences, Medical Faculty, Saarland University, Homburg, Germany"},{"name":"Dynamics of Fluids, Experimental Physics, Saarland University, Saarbr\u00fccken, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Connes","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 EA7424, Team Vascular Biology and Red Blood Cell, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, Lyon, France"},{"name":"Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"issue":"03","key":"ref1","first-page":"1242","article-title":"Deformability of heterogeneous red blood cells in aging and related pathologies","volume":"16","author":"D S Prudinnik","year":"2024","journal-title":"Aging Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.3389\/fphys.2019.01329","article-title":"Blood rheology: key parameters, impact on blood flow, role in sickle cell disease and effects of exercise","volume":"10","author":"E Nader","year":"2019","journal-title":"Front Physiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"655393","DOI":"10.3389\/fphys.2021.655393","article-title":"How do red blood cells die?","volume":"12","author":"P Thiagarajan","year":"2021","journal-title":"Front Physiol"},{"issue":"16","key":"ref4","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.1016\/j.bpj.2025.07.002","article-title":"Viscosity and density measurements on the cytosol of human red blood cells","volume":"124","author":"T John","year":"2025","journal-title":"Biophys J"},{"issue":"05","key":"ref5","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1182\/blood.V61.5.899.899","article-title":"Osmotic gradient ektacytometry: comprehensive characterization of red cell volume and surface maintenance","volume":"61","author":"M R Clark","year":"1983","journal-title":"Blood"},{"issue":"16","key":"ref6","doi-asserted-by":"crossref","first-page":"12755","DOI":"10.3390\/ijms241612755","article-title":"Red blood cell deformability is expressed by a set of interrelated membrane proteins","volume":"24","author":"G Barshtein","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"09","key":"ref7","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1182\/blood-2012-04-422253","article-title":"Mutations in the mechanotransduction protein PIEZO1 are associated with hereditary xerocytosis","volume":"120","author":"R Zarychanski","year":"2012","journal-title":"Blood"},{"issue":"19","key":"ref8","doi-asserted-by":"crossref","first-page":"3925","DOI":"10.1182\/blood-2013-02-482489","article-title":"Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO1","volume":"121","author":"I Andolfo","year":"2013","journal-title":"Blood"},{"issue":"01","key":"ref9","doi-asserted-by":"crossref","first-page":"70","DOI":"10.3324\/haematol.2013.086090","article-title":"Piezo1 plays a role in erythrocyte volume homeostasis","volume":"99","author":"A Faucherre","year":"2014","journal-title":"Haematologica"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"e07370","DOI":"10.7554\/eLife.07370","article-title":"Piezo1 links mechanical forces to red blood cell volume","volume":"4","author":"S M Cahalan","year":"2015","journal-title":"eLife"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1222983","DOI":"10.3389\/fphys.2023.1222983","article-title":"Dual action of Dooku1 on PIEZO1 channel in human red blood cells","volume":"14","author":"A Hatem","year":"2023","journal-title":"Front Physiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nature13701","article-title":"Piezo1 integration of vascular architecture with physiological force","volume":"515","author":"J Li","year":"2014","journal-title":"Nature"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.1038\/ncomms2899","article-title":"Dehydrated hereditary stomatocytosis linked to gain-of-function mutations in mechanically activated PIEZO1 ion channels","volume":"4","author":"J Albuisson","year":"2013","journal-title":"Nat Commun"},{"issue":"11","key":"ref14","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1182\/blood-2015-07-657957","article-title":"Mutations in the Gardos channel (KCNN4) are associated with hereditary xerocytosis","volume":"126","author":"E Glogowska","year":"2015","journal-title":"Blood"},{"issue":"08","key":"ref15","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.3324\/haematol.2018.205328","article-title":"Clinical and biological features in PIEZO1-hereditary xerocytosis and Gardos channelopathy: a retrospective series of 126 patients","volume":"104","author":"V Picard","year":"2019","journal-title":"Haematologica"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.86152","volume-title":"Red blood cells actively contribute to blood coagulation and thrombus formation","author":"I Bernhardt","year":"2019"},{"issue":"09","key":"ref17","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1001\/jama.1983.03340090057031","article-title":"The relation of blood platelets to hemorrhagic disease: description of a method for determining the bleeding time and coagulation time and report of three cases of hemorrhagic disease relieved by transfusion","volume":"250","author":"W W Duke","year":"1983","journal-title":"JAMA"},{"issue":"01","key":"ref18","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/j.1365-2141.1961.tb00318.x","article-title":"The role of red cells in haemostasis: the relation between haematocrit, bleeding time and platelet adhesiveness","volume":"7","author":"A J Hellem","year":"1961","journal-title":"Br J Haematol"},{"issue":"31","key":"ref19","doi-asserted-by":"crossref","first-page":"18651","DOI":"10.1074\/jbc.271.31.18651","article-title":"Prostaglandin E2 stimulates a Ca2+-dependent K+ channel in human erythrocytes and alters cell volume and filterability","volume":"271","author":"Q Li","year":"1996","journal-title":"J Biol Chem"},{"issue":"07","key":"ref20","doi-asserted-by":"crossref","first-page":"2420","DOI":"10.1182\/blood.V95.7.2420","article-title":"Lysophosphatidic acid opens a Ca(++) channel in human erythrocytes","volume":"95","author":"L Yang","year":"2000","journal-title":"Blood"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S1567-5394(01)00164-5","article-title":"Ion channels in the human red blood cell membrane: their further investigation and physiological relevance","volume":"55","author":"L Kaestner","year":"2002","journal-title":"Bioelectrochemistry"},{"issue":"06","key":"ref22","first-page":"1269","article-title":"Prostaglandin E2 activates channel-mediated calcium entry in human erythrocytes: an indication for a blood clot formation supporting process","volume":"92","author":"L Kaestner","year":"2004","journal-title":"Thromb Haemost"},{"issue":"01","key":"ref23","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ceca.2011.05.002","article-title":"Stimulation of human red blood cells leads to Ca2+-mediated intercellular adhesion","volume":"50","author":"P Steffen","year":"2011","journal-title":"Cell Calcium"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.bioelechem.2011.08.004","article-title":"Lysophosphatidic acid induced red blood cell aggregation in vitro","volume":"87","author":"L Kaestner","year":"2012","journal-title":"Bioelectrochemistry"},{"issue":"09","key":"ref25","doi-asserted-by":"crossref","first-page":"2418","DOI":"10.1016\/j.jtha.2023.05.022","article-title":"Activation of Piezo1 channels in compressed red blood cells augments platelet-driven contraction of blood clots","volume":"21","author":"N G Evtugina","year":"2023","journal-title":"J Thromb Haemost"},{"issue":"17","key":"ref26","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1182\/bloodadvances.2020003823","article-title":"Mechanistic ion channel interactions in red cells of patients with G\u00e1rdos channelopathy","volume":"5","author":"J Jansen","year":"2021","journal-title":"Blood Adv"},{"issue":"02","key":"ref27","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/0005-2736(78)90253-5","article-title":"Erythrocyte membrane potentials determined by hydrogen ion distribution","volume":"512","author":"R I Macey","year":"1978","journal-title":"Biochim Biophys Acta"},{"issue":"03","key":"ref28","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/BF01871176","article-title":"Flux ratio of valinomycin-mediated K+ fluxes across the human red cell membrane in the presence of the protonophore CCCP","volume":"93","author":"P Bennekou","year":"1986","journal-title":"J Membr Biol"},{"issue":"24","key":"ref29","doi-asserted-by":"crossref","first-page":"6336","DOI":"10.1182\/bloodadvances.2020003285","article-title":"Gardos channelopathy: functional analysis of a novel KCNN4 variant","volume":"4","author":"E Fermo","year":"2020","journal-title":"Blood Adv"},{"issue":"03","key":"ref30","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1111\/bjh.18799","article-title":"Piezo1 activation augments sickling propensity and the adhesive properties of sickle red blood cells in a calcium-dependent manner","volume":"202","author":"E Nader","year":"2023","journal-title":"Br J Haematol"},{"issue":"02","key":"ref32","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3233\/CH-2009-1202","article-title":"New guidelines for hemorheological laboratory techniques","volume":"42","author":"O K Baskurt","year":"2009","journal-title":"Clin Hemorheol Microcirc"},{"issue":"01","key":"ref33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/(SICI)1097-0320(19980101)31:1<1::AID-CYTO1>3.0.CO;2-R","article-title":"Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure","volume":"31","author":"M van Engeland","year":"1998","journal-title":"Cytometry"},{"issue":"02","key":"ref34","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1182\/blood.2020004853","article-title":"Plasma microparticles of sickle patients during crisis or taking hydroxyurea modify endothelium inflammatory properties","volume":"136","author":"Y Garnier","year":"2020","journal-title":"Blood"},{"issue":"03","key":"ref35","doi-asserted-by":"crossref","first-page":"658","DOI":"10.3390\/diagnostics12030658","article-title":"The non-activated thromboelastometry (NATEM) assay's application among adults and neonatal\/pediatric population: a systematic review","volume":"12","author":"P Georgiadou","year":"2022","journal-title":"Diagnostics (Basel)"},{"issue":"09","key":"ref36","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1111\/jth.15016","article-title":"Hypofibrinolytic state and high thrombin generation may play a major role in SARS-COV2 associated thrombosis","volume":"18","author":"C Nougier","year":"2020","journal-title":"J Thromb Haemost"},{"issue":"22","key":"ref37","doi-asserted-by":"crossref","first-page":"8531","DOI":"10.3390\/ijms21228531","article-title":"Impact of trail running races on blood viscosity and its determinants: effects of distance","volume":"21","author":"M Robert","year":"2020","journal-title":"Int J Mol Sci"},{"issue":"01","key":"ref38","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1136\/jcp.21.1.110","article-title":"Rapid method for the estimation of plasma haemoglobin levels","volume":"21","author":"C M Cripps","year":"1968","journal-title":"J Clin Pathol"},{"issue":"36","key":"ref39","doi-asserted-by":"crossref","first-page":"e2407765121","DOI":"10.1073\/pnas.2407765121","article-title":"Lysis of human erythrocytes due to Piezo1-dependent cytosolic calcium overload as a mechanism of circulatory removal","volume":"121","author":"L Kuck","year":"2024","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"ref40","doi-asserted-by":"crossref","first-page":"3113","DOI":"10.1111\/jth.15504","article-title":"Piezo1 initiates platelet hyperreactivity and accelerates thrombosis in hypertension","volume":"19","author":"W Zhao","year":"2021","journal-title":"J Thromb Haemost"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1101\/2025.05.27.656389","article-title":"Targeting PIEZO1-TMEM16F coupling to mitigate sickle cell disease complications","author":"P Liang","journal-title":"bioRxiv"},{"issue":"04","key":"ref42","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1182\/blood.2023021465","article-title":"Deciphering and disrupting PIEZO1-TMEM16F interplay in hereditary xerocytosis","volume":"143","author":"P Liang","year":"2024","journal-title":"Blood"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"673","DOI":"10.3389\/fphys.2017.00673","article-title":"Is increased intracellular calcium in red blood cells a common component in the molecular mechanism causing anemia?","volume":"8","author":"L Hertz","year":"2017","journal-title":"Front Physiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"551441","DOI":"10.3389\/fimmu.2020.551441","article-title":"Association between nitric oxide, oxidative stress, eryptosis, red blood cell microparticles, and vascular function in sickle cell anemia","volume":"11","author":"E Nader","year":"2020","journal-title":"Front Immunol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/246105a0","article-title":"Elevated erythrocyte calcium in sickle cell disease","volume":"246","author":"J W Eaton","year":"1973","journal-title":"Nature"},{"issue":"27","key":"ref46","doi-asserted-by":"crossref","first-page":"6355","DOI":"10.1021\/bi00142a027","article-title":"Ca2+ induces transbilayer redistribution of all major phospholipids in human erythrocytes","volume":"31","author":"P Williamson","year":"1992","journal-title":"Biochemistry"},{"issue":"05","key":"ref47","doi-asserted-by":"crossref","first-page":"9848","DOI":"10.3390\/ijms14059848","article-title":"Calcium in red blood cells-a perilous balance","volume":"14","author":"A Bogdanova","year":"2013","journal-title":"Int J Mol Sci"},{"issue":"05","key":"ref48","doi-asserted-by":"crossref","first-page":"e179","DOI":"10.3324\/haematol.2018.201160","article-title":"A novel gain-of-function mutation of Piezo1 is functionally affirmed in red blood cells by high-throughput patch clamp","volume":"104","author":"M G Rotordam","year":"2019","journal-title":"Haematologica"},{"issue":"23","key":"ref49","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1182\/blood.2019000424","article-title":"Red blood cells modulate structure and dynamics of venous clot formation in sickle cell disease","volume":"133","author":"C Faes","year":"2019","journal-title":"Blood"},{"issue":"S 06","key":"ref50","first-page":"S1","article-title":"Basic principles of viscoelastic testing","volume":"60","author":"T Carll","year":"2020","journal-title":"Transfusion"},{"issue":"08","key":"ref51","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1111\/jth.15407","article-title":"Pathologically stiff erythrocytes impede contraction of blood clots","volume":"19","author":"V Tutwiler","year":"2021","journal-title":"J Thromb Haemost"},{"issue":"01","key":"ref52","doi-asserted-by":"crossref","first-page":"100023","DOI":"10.1016\/j.rpth.2022.100023","article-title":"Blood clot contraction: mechanisms, pathophysiology, and disease","volume":"7","author":"R I Litvinov","year":"2022","journal-title":"Res Pract Thromb Haemost"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3233\/CH-238122","article-title":"Blood rheology and vascular function in sickle cell trait and sickle cell disease: from pathophysiological mechanisms to clinical usefulness","volume":"86","author":"P Connes","year":"2024","journal-title":"Clin Hemorheol Microcirc"},{"issue":"01","key":"ref54","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1038\/s41598-017-01591-w","article-title":"'Gardos channelopathy': a variant of hereditary stomatocytosis with complex molecular regulation","volume":"7","author":"E Fermo","year":"2017","journal-title":"Sci Rep"},{"issue":"06","key":"ref55","doi-asserted-by":"crossref","first-page":"482","DOI":"10.3390\/cells13060482","article-title":"Piezo1 and its function in different blood cell lineages","volume":"13","author":"A I Karkempetzaki","year":"2024","journal-title":"Cells"}],"container-title":["Seminars in Thrombosis and Hemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2846-3643.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T23:03:56Z","timestamp":1782255836000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2846-3643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,1]]},"references-count":54,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2026,6,23]]},"published-print":{"date-parts":[[2026,7]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2846-3643","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0094-6176","1098-9064"],"issn-type":[{"value":"0094-6176","type":"print"},{"value":"1098-9064","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,4,1]]},"published":{"date-parts":[[2026,4,1]]}}}