{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:51:36Z","timestamp":1791589896093,"version":"4.3.4"},"reference-count":17,"publisher":"Georg Thieme Verlag KG","issue":"05","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tierarztl Prax Ausg K Kleintiere Heimtiere"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title>\n                  <jats:p>Die zuverl\u00e4ssige Bestimmung von Steroidhormonen wie 17-Hydroxyprogesteron\nwird seit langem durch die Limitierungen immunologischer Verfahren\nerschwert. Assays wie Enzyme-linked Immunosorbent Assay (ELISA),\nRadioimmunoassay (RIA) oder auch Chemilumineszenz-Immunoassay (CLIA) zeigen\nje nach Test erhebliche Kreuzreaktionen, Interferenzen und eine ausgepr\u00e4gte\nVariabilit\u00e4t, wodurch ihre diagnostische Aussagekraft eingeschr\u00e4nkt ist.<\/jats:p>\n                  <jats:p>Ziel dieser Arbeit war die methodische Etablierung und Validierung einer\nLC-MS\/MS-basierten Bestimmung von 17-Hydroxyprogesteron, um eine spezifische\nund robuste Alternative zu immunologischen Testsystemen bereitzustellen.<\/jats:p>\n                  <jats:p>Die Entwicklung der Methode erfolgte unter Verwendung stabiler\nIsotopenstandards und umfasste die systematische Pr\u00fcfung von Linearit\u00e4t,\nSensitivit\u00e4t, Pr\u00e4zision, Wiederfindung und Matrixeffekten. Im Anschluss\nwurden erste Serumproben von Hunden im Rahmen der Routinediagnostik\nuntersucht, um die Anwendbarkeit im veterin\u00e4rmedizinischen Setting\nexemplarisch zu illustrieren.<\/jats:p>\n                  <jats:p>Das Verfahren zeigte eine ausgezeichnete analytische Performance mit einer\nNachweisgrenze von 0,044 ng\/ml, hoher Linearit\u00e4t (R\u00b2&gt;0,999) und geringen\nVariationskoeffizienten (&lt;5%). Kreuzreaktionen, wie sie bei\nimmunologischen Systemen beobachtet werden, konnten ausgeschlossen\nwerden.<\/jats:p>\n                  <jats:p>Mit der Etablierung der LC-MS\/MS-basierten 17-Hydroxyprogesteron-Bestimmung\nsteht im Gegensatz zu immunologisch basierten Testsystemen ein\nhochspezifisches und pr\u00e4zises Verfahren zur Verf\u00fcgung. Es bietet somit eine\nbelastbare Grundlage f\u00fcr die endokrinologische Diagnostik der\nSteroidhormonanalytik beim Hund.<\/jats:p>","DOI":"10.1055\/a-2854-7332","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T23:19:57Z","timestamp":1787181597000},"page":"344-349","source":"Crossref","is-referenced-by-count":0,"title":["Etablierung und Validierung einer LC-MS\/MS-basierten Bestimmung von\n17-Hydroxyprogesteron beim Hund"],"prefix":"10.1055","volume":"54","author":[{"given":"Sebastian","family":"Naczenski","sequence":"additional","affiliation":[{"name":"Laboklin GmbH & Co. KG, Bad Kissingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Klein","sequence":"additional","affiliation":[{"name":"Laboklin GmbH & Co. KG, Bad Kissingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Hildebrand","sequence":"additional","affiliation":[{"name":"Laboklin GmbH & Co. KG, Bad Kissingen"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1186\/1472-6890-14-33","article-title":"Cross-reactivity of steroid hormone immunoassays: clinical significance and\ntwo-dimensional molecular similarity prediction","volume":"14","author":"M D Krasowski","year":"2014","journal-title":"BMC Clin Pathol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1515\/cclm-2023-0344","article-title":"Methodological considerations in determining sex steroids in children:\ncomparison of conventional immunoassays with liquid chromatography-tandem mass\nspectrometry","volume":"62","author":"C Ankarberg-Lindgren","year":"2024","journal-title":"Clin Chem Lab Med (CCLM)"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1016\/j.clinbiochem.2016.04.009","article-title":"LC\u2013MS\/MS analysis of steroids in the clinical laboratory","volume":"49","author":"B G Keevil","year":"2016","journal-title":"Clin Biochem"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1634","DOI":"10.1292\/jvms.21-0406","article-title":"Simultaneous steroids measurement in dogs with hyperadrenocorticism using a\ncolumn-switching liquid chromatography-tandem mass spectrometry method","volume":"83","author":"N Sasaki","year":"2021","journal-title":"J Vet Med Sci"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1136\/vr.162.21.673","article-title":"Evaluation of cortisol precursors for the diagnosis of pituitary-dependent\nhypercortisolism in dogs","volume":"162","author":"N S Sieber-Ruckstuhl","year":"2008","journal-title":"Vet Rec"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.2460\/javma.2005.227.1095","article-title":"Evaluation of serum 17-hydroxyprogesterone concentration after administration of\nACTH in dogs with hyperadrenocorticism","volume":"227","author":"N Benitah","year":"2005","journal-title":"J Am Vet Med Assoc"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1892\/0891-6640(2002)016<0433:tuoitd>2.3.co;2","article-title":"The Use of 17-Hydroxyprogesterone in the Diagnosis of Canine\nHyperadrenocorticism","volume":"16","author":"JM E Ristic","year":"2002","journal-title":"J Vet Int Med"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1292","DOI":"10.1111\/jvim.12192","article-title":"Diagnosis of Spontaneous Canine Hyperadrenocorticism: 2012 ACVIM Consensus\nStatement (Small Animal)","volume":"27","author":"E N Behrend","year":"2013","journal-title":"J Vet Intern Med"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.cvsm.2009.11.002","article-title":"Atypical Cushing\u2019s Syndrome in Dogs: Arguments For and Against","volume":"40","author":"E N Behrend","year":"2010","journal-title":"Vet Clin North Am Small Anim Pract"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"956","DOI":"10.3390\/jcm11040956","article-title":"Rapidity and Precision of Steroid Hormone Measurement","volume":"11","author":"S Karashima","year":"2022","journal-title":"JCM"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"D1","DOI":"10.1530\/EJE-15-0338","article-title":"Mass spectrometry and immunoassay: how to measure steroid hormones today and\ntomorrow","volume":"173","author":"A E Taylor","year":"2015","journal-title":"Eur J Endocrinol"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.jsbmb.2010.05.001","article-title":"Advantages and challenges of mass spectrometry assays for steroid hormones","volume":"121","author":"F Z Stanczyk","year":"2010","journal-title":"J Steroid Biochem Mol Biol"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1111\/vcp.12301","article-title":"Liquid chromatography-tandem mass spectrometry ( LC \u2013 MS \/ MS ) for analysis of\nendogenous steroids in the luteal phase and early pregnancy in dogs: a pilot\nstudy","volume":"44","author":"B S Holst","year":"2015","journal-title":"Vet Clin Pathol"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"2152","DOI":"10.1111\/jvim.16224","article-title":"Multiple corticosteroid abnormalities in cats with hyperaldosteronism","volume":"35","author":"D K Langlois","year":"2021","journal-title":"J Vet Intern Med"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1111\/vcp.12958","article-title":"Patterns of the low-dose dexamethasone suppression test in canine\nhyperadrenocorticism revisited","volume":"50","author":"F K Zeugswetter","year":"2021","journal-title":"Vet Clin Pathol"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1111\/jvim.12500","article-title":"Serum Cortisol Concentrations in Dogs with Pituitary-Dependent\nHyperadrenocorticism and Atypical Hyperadrenocorticism","volume":"29","author":"L A Frank","year":"2015","journal-title":"J Vet Intern Med"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.1014792","article-title":"Serum steroid profiling of hepatocellular carcinoma associated with\nhyperadrenocorticism in dogs: A preliminary study","volume":"9","author":"T Oo","year":"2022","journal-title":"Front Vet Sci"}],"container-title":["Tier\u00e4rztliche Praxis Ausgabe K: Kleintiere \/ Heimtiere"],"original-title":[],"language":"de","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2854-7332.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:22:16Z","timestamp":1791588136000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2854-7332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":17,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2026,10,9]]},"published-print":{"date-parts":[[2026,10]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2854-7332","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["1434-1239","2567-5842"],"issn-type":[{"value":"1434-1239","type":"print"},{"value":"2567-5842","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}