{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T23:57:51Z","timestamp":1785455871359,"version":"3.56.0"},"reference-count":32,"publisher":"Georg Thieme Verlag KG","funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de\n                        Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","award":["Code 001"],"award-info":[{"award-number":["Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento\n                        Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["310407\/2022-2"],"award-info":[{"award-number":["310407\/2022-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Helicobacter pylori infection is related to several gastric pathologies,\n                    including gastritis, peptic ulcer, and gastric cancer. Substances capable of\n                    eradicating H. pylori have been subject to the attention of researchers.\n                    This study evaluated a fully characterized ethyl acetate fraction, obtained from\n                    the rhizomes of Limonium brasiliense, and its microparticulate\n                    pharmaceutical form against H. pylori, as well as their potential\n                    immunomodulatory and antitumor activities associated with H. pylori\n                    infection. Ultra-high-performance liquid chromatography analysis of ethyl\n                    acetate fraction indicated the presence of samaragenins A and B along with\n                    gallic acid, epigallocatechin, and epigallocatechin-3-O-gallate.\n                    Antioxidant activity showed half-maximum inhibitory concentration values of 5.7\n                    (95% confidence interval: 5.21\u200a\u2013\u200a6.83), 31.9 (95% confidence interval:\n                    19.57\u200a\u2013\u200a51.99), and 33.1 (95% confidence interval: 24.4\u200a\u2013\u200a44.73) \u00b5g\/mL for HOCl,\n                        O2\n               \u2022\u2212, and NO, respectively. Ethyl acetate fraction\n                    exerted minimal inhibitory concentration values of 1,024 and 128\u2009\u00b5g\/mL against\n                    the H. pylori (ATCC 43\u200a504, clinical isolate H. pylori strain).\n                        In vivo treatments in mice with ethyl acetate fraction and\n                    microparticulate ethyl acetate fraction (100\u2009mg\/kg, in single daily doses for 20\n                    consecutive days) improved gastric pathology in infected animals, reducing\n                    urease positivity (14.3% vs. 54.5% in untreated controls), inflammation (tumor\n                    necrosis factor alpha, interleukin 1\u03b2, and interleukin 6 reduced,\n                    p\u2009&lt;\u20090.05), and morphological damage compared with the untreated control\n                    group. H. pylori urease was inhibited with half-maximum inhibitory\n                    concentration of 521.6\u2009\u00b5g\/mL (95% confidence interval: 419.2\u200a\u2013\u200a649.0). In\n                    addition, ethyl acetate fraction showed antiproliferative activity against\n                    gastric adenocarcinoma cells (half cytotoxic concentration of 21.7, 95%\n                    confidence interval: 18.1\u200a\u2013\u200a26.04). In Escherichia coli\n                    lipopolysaccharide-stimulated macrophages, ethyl acetate fraction reduced the\n                    tumor necrosis factor alpha and interleukin 6 production by 16% and 35%,\n                    respectively. These results indicate the potential of L.\u00a0brasiliense as a\n                    source of compounds capable of attenuating gastric diseases related to H.\n                        pylori infection.<\/jats:p>","DOI":"10.1055\/a-2897-9285","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T22:49:09Z","timestamp":1781822949000},"source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Potential of Limonium brasiliense Extracts in\n                        Helicobacter pylori-Associated Gastric Pathologies"],"prefix":"10.1055","author":[{"given":"Augusto Santos","family":"Borges","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Laboratory of Pharmaceutical Biology, Palafito,\n                    Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina","family":"Guidi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Laboratory of Pharmaceutical Biology, Palafito,\n                    Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Nascimento de","family":"Paula","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Laboratory of Pharmaceutical Biology, Palafito,\n                    Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloisa Lorenzi da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Laboratory of Pharmaceutical Biology, Palafito,\n                    Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Jorge","family":"Fagundes","sequence":"additional","affiliation":[{"name":"Department of Medicine, Universidade Estadual de Maring\u00e1, Maring\u00e1,\n                    Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Aur\u00e9lio Valad\u00e3o","family":"Fagundes","sequence":"additional","affiliation":[{"name":"Department of Medicine, Universidade Estadual de Maring\u00e1, Maring\u00e1,\n                    Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Rezende","family":"Kitagawa","sequence":"additional","affiliation":[{"name":"Graduate Program in Pharmaceutical Sciences, Universidade Federal do\n                    Esp\u00edrito Santo, Vitoria, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita de C\u00e1ssia Ribeiro","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Graduate Program in Pharmaceutical Sciences, Universidade Federal do\n                    Esp\u00edrito Santo, Vitoria, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Cristina de Medeiros","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Laboratory of Pharmaceutical Biology, Palafito,\n                    Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7532-3395","authenticated-orcid":false,"given":"Jo\u00e3o Carlos Palazzo de","family":"Mello","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Laboratory of Pharmaceutical Biology, Palafito,\n                    Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/j.jcmgh.2016.12.001","article-title":"The clinical evidence linking Helicobacter pylori to gastric cancer","volume":"3","author":"S F Moss","year":"2017","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"ref2","first-page":"591","article-title":"Risk factors for gastric cancer: A systematic review","volume":"19","author":"A R Yusefi","year":"2018","journal-title":"Asian Pac J Cancer Prev"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"229","DOI":"10.3322\/caac.21834","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality\n                    worldwide for 36 cancers in 185 countries","volume":"74","author":"F Bray","year":"2024","journal-title":"CA Cancer J Clin"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1053\/j.gastro.2016.01.028","article-title":"Association between Helicobacter pylori eradication and gastric cancer\n                    incidence: A systematic review and meta-analysis","volume":"150","author":"Y C Lee","year":"2016","journal-title":"Gastroenterology"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1111\/hel.12163","article-title":"Treatment of Helicobacter pylori infection 2014","volume":"19","author":"A O\u02bcConnor","year":"2014","journal-title":"Helicobacter"},{"key":"ref6","first-page":"45","article-title":"Estudos farmacol\u00f3gicos preliminares das ra\u00edzes do Limonium brasiliense\n                    (Boiss.) Kuntze \u2013 Plumbaginaceae (baicuru)*","volume":"1","author":"T FAL Moura","year":"1985","journal-title":"Cad Farm"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1515\/znc-2004-7-804","article-title":"Antioxidant metabolites from Limonium brasiliense (Boiss.) 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Blainski","year":"2017","journal-title":"Rev Bras Farmacogn"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.fitote.2017.03.002","article-title":"Flavan-3-ols and proanthocyanidins from Limonium brasiliense inhibit the\n                    adhesion of Porphyromonas gingivalis to epithelial host cells by\n                    interaction with gingipains","volume":"118","author":"A de Oliveira Caleare","year":"2017","journal-title":"Fitoterapia"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1590\/S0074-02762002000700017","article-title":"Screening of some plants used in the Brazilian folk medicine for the treatment\n                    of infectious diseases","volume":"97","author":"F B Holetz","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"10368","DOI":"10.3748\/wjg.v20.i30.10368","article-title":"Medicinal plant activity on Helicobacter pylori related diseases","volume":"20","author":"Y C Wang","year":"2014","journal-title":"World J Gastroenterol"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"112207","DOI":"10.1016\/j.indcrop.2020.112207","article-title":"Plectranthus barbatus Andrews as anti-Helicobacter pylori agent\n                    with activity against adenocarcinoma gastric cells","volume":"146","author":"A S Borges","year":"2020","journal-title":"Ind Crops Prod"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.copbio.2011.08.007","article-title":"Polyphenols as antimicrobial agents","volume":"23","author":"M Daglia","year":"2012","journal-title":"Curr Opin Biotechnol"},{"key":"ref18","first-page":"2470","article-title":"Essential role of urease in pathogenesis of gastritis induced by Helicobacter\n                        pylori in gnotobiotic piglets","volume":"59","author":"K A Eaton","year":"1991","journal-title":"Rev Infect Dis"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"2245","DOI":"10.1016\/j.bbagen.2018.07.020","article-title":"Targeting Helicobacter pylori urease activity and maturation: In-cell\n                    high-throughput approach for drug discovery","volume":"1862","author":"C Tarsia","year":"2018","journal-title":"Biochim Biophys Acta Gen Subj"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1007\/s12275-015-5085-5","article-title":"Morphological changes in human gastric epithelial cells induced by nuclear\n                    targeting of Helicobacter pylori urease subunit A","volume":"53","author":"J H Lee","year":"2015","journal-title":"J Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.3748\/wjg.v16.i10.1188","article-title":"Influence of interleukin polymorphisms on development of gastric cancer and\n                    peptic ulcer","volume":"16","author":"M Sugimoto","year":"2010","journal-title":"World 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El-Omar","year":"2003","journal-title":"Gastroenterology"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"3658","DOI":"10.4049\/jimmunol.174.6.3658","article-title":"Helicobacter pylori disrupts NADPH oxidase targeting in human neutrophils\n                    to induce extracellular superoxide release","volume":"174","author":"L A Allen","year":"2005","journal-title":"J Immunol"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1016\/j.fitote.2011.01.018","article-title":"Antioxidant and prooxidant properties of flavonoids","volume":"82","author":"D Proch\u00e1zkov\u00e1","year":"2011","journal-title":"Fitoterapia"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1248\/cpb.37.3229","article-title":"Superoxide anion scavenging properties of fluvastatin and its metabolites","volume":"47","author":"K Suzumura","year":"1999","journal-title":"Chem Pharm Bull (Tokyo)"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1006\/abio.1994.1195","article-title":"A method for screening hypochlorous acid scavengers by inhibition of the\n                    oxidation of 5-thio-2-nitrobenzoic acid: application to anti-asthmatic\n                    drugs","volume":"218","author":"T L Ching","year":"1994","journal-title":"Anal Biochem"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to\n                    proliferation and cytotoxicity assays","volume":"65","author":"T Mosmann","year":"1983","journal-title":"J Immunol Methods"},{"key":"ref30","volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow\n                    Aerobically; Approved Standard\u20139th ed. CLSI document M07-A9","author":"Clinical and Laboratory Standards Institute\n                            (CLSI)","year":"2012"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"7172","DOI":"10.1021\/jf100274g","article-title":"In vitro and in vivo effects of apple peel polyphenols against\n                        Helicobacter pylori","volume":"58","author":"E Pastene","year":"2010","journal-title":"J Agric Food Chem"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.jep.2016.06.032","article-title":"Anti-ulcer mechanisms of polyphenols extract of Euphorbia umbellata (Pax)\n                    Bruyns (Euphorbiaceae)","volume":"191","author":"B R Minozzo","year":"2016","journal-title":"J Ethnopharmacol"}],"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2897-9285.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T23:00:58Z","timestamp":1785452458000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2897-9285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,18]]},"references-count":32,"URL":"https:\/\/doi.org\/10.1055\/a-2897-9285","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"value":"0032-0943","type":"print"},{"value":"1439-0221","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,6,18]]},"published":{"date-parts":[[2026,6,18]]}}}