{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:20:46Z","timestamp":1790281246798,"version":"4.1.0"},"reference-count":39,"publisher":"Georg Thieme Verlag KG","issue":"07","funder":[{"name":"Wannan Medical\nCollege","award":["KF2024006, KF2024008"],"award-info":[{"award-number":["KF2024006, KF2024008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Res (Stuttg)"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Astragalus polysaccharide (APS) has therapeutic potential for\nneurodegenerative diseases; however, its specific mechanism of action\nagainst ischemic stroke (IS) requires further elucidation. This study aimed\nto elucidate the protective effects of APS on neural tissue and to explore\nthe underlying molecular pathways in a rat population subjected to middle\ncerebral artery occlusion (MCAO)<\/jats:p>\n                  <jats:p>The animals received intraperitoneal (IP) injections of APS. Neurological\nrecovery was assessed using neurobehavioral tests, and neuronal morphology\nwas examined using Nissl staining. Then, we assessed the mRNA levels of\nIL-1\u03b2, IL-6, and TNF-\u03b1 as well as the concentrations of oxidative\nstress-related substances. Finally, we evaluated the expression levels of\nNrf2, Keap1, HO-1, and GPX4 through Western blotting and\nimmunofluorescence.<\/jats:p>\n                  <jats:p>APS improved neurological function and ameliorated neuronal damage. It\nconcurrently inhibited IL-1\u03b2, IL-6, and TNF-\u03b1 expression while enhancing\nantioxidative potential. Mechanistically, APS induced an elevation in the\nlevels of Nrf2, HO-1, and GPX4 proteins while concurrently causing a\nreduction in the protein levels of Keap1 in the striatum.<\/jats:p>\n                  <jats:p>APS effectively mitigated neuronal damage and motor dysfunction after\ncerebral ischemia. The protective mechanism involves the activation of the\nNrf2\/HO-1 axis and subsequent suppression of oxidative stress and\nneuroinflammation.<\/jats:p>","DOI":"10.1055\/a-2898-0240","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T23:15:16Z","timestamp":1783984516000},"page":"195-204","source":"Crossref","is-referenced-by-count":1,"title":["Astragalus Polysaccharide Alleviates Oxidative Stress Injury through\nModulation of the Nrf2\/HO-1 Axis in the Striatum of Cerebral Ischemic\nRats"],"prefix":"10.1055","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2868-628X","authenticated-orcid":false,"given":"Qi","family":"Wu","sequence":"first","affiliation":[{"name":"Wannan Medical College, An Hui, China","place":["Wuhu"],"department":["School of Basic Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinzhong","family":"Ni","sequence":"first","affiliation":[{"name":"Wannan Medical College, An Hui, China","place":["Wuhu"],"department":["School of Basic Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Teng","sequence":"additional","affiliation":[{"name":"Wannan Medical College, An Hui, China","place":["Wuhu"],"department":["School of Basic Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Yang","sequence":"additional","affiliation":[{"name":"Wannan Medical College, An Hui, China","place":["Wuhu"],"department":["School of Basic Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Li","sequence":"additional","affiliation":[{"name":"Wannan Medical College, An Hui, China","place":["Wuhu"],"department":["School of Basic Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9528-5541","authenticated-orcid":false,"given":"Huachun","family":"Miao","sequence":"additional","affiliation":[{"name":"Wannan Medical College, An Hui, China","place":["Wuhu"],"department":["School of Basic Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaibin","family":"Li","sequence":"additional","affiliation":[{"name":"Wannan Medical College, An Hui, China","place":["Wuhu"],"department":["School of Basic Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"105458","DOI":"10.1016\/j.neuint.2022.105458","article-title":"Stroke: Molecular mechanisms and therapies: Update on recent developments","volume":"162","author":"F Shehjar","year":"2023","journal-title":"Neurochem Int"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"e00993","DOI":"10.1002\/brb3.993","article-title":"Nest-building activity as a reproducible and long-term stroke deficit test in a\nmouse model of stroke","volume":"8","author":"D Yuan","year":"2018","journal-title":"Brain Behav"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.neuron.2008.11.005","article-title":"Striatal plasticity and basal ganglia circuit function","volume":"60","author":"A C Kreitzer","year":"2008","journal-title":"Neuron"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.nbd.2016.11.005","article-title":"Optogenetic stimulation of glutamatergic neuronal activity in the striatum\nenhances neurogenesis in the subventricular zone of normal and stroke mice","volume":"98","author":"M Song","year":"2017","journal-title":"Neurobiol Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1007\/s10072-017-2938-1","article-title":"Pathogenic mechanisms following ischemic stroke","volume":"38","author":"S E Khoshnam","year":"2017","journal-title":"Neurol Sci"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1007\/s11064-024-04185-x","article-title":"Electroacupuncture downregulating neuronal ferroptosis in MCAO\/R rats by\nActivating Nrf2\/SLC7A11\/GPX4 Axis","volume":"49","author":"W Zhu","year":"2024","journal-title":"Neurochem Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1728908","DOI":"10.3389\/fphar.2025.1728908","article-title":"Therapeutic effects of Lactobacillus rhamnosus, thymol and their\ncombination against neurotoxicity in propionic acid (PA)-induced autistic rats:\ninsights into the role of the Nrf2\/HO-1, Wnt3\/\u03b2-catenin\/GSK3\u03b2 BDNF\/p-TrkB\/CREB,\npI3K\/Akt\/mTOR, AMPK\/SIRT-1, and 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Mol Life Sci"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"102852","DOI":"10.1016\/j.redox.2023.102852","article-title":"Remote ischemic conditioning attenuates oxidative stress and inflammation via\nthe Nrf2\/HO-1 pathway in MCAO mice","volume":"66","author":"Y Y Sun","year":"2023","journal-title":"Redox Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2621","DOI":"10.1080\/15548627.2023.2218764","article-title":"GPX4 in cell death, autophagy, and disease","volume":"19","author":"Y Xie","year":"2023","journal-title":"Autophagy"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1449101","DOI":"10.3389\/fphar.2024.1449101","article-title":"Pharmacological effects of Astragalus polysaccharides in\ntreating neurodegenerative diseases","volume":"15","author":"Y Shi","year":"2024","journal-title":"Front\nPharmacol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"165260","DOI":"10.1016\/j.bbadis.2018.09.012","article-title":"Current advances in ischemic stroke research and therapies","volume":"1866","author":"D Barthels","year":"2020","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1161\/01.STR.20.1.84","article-title":"Reversible middle cerebral artery occlusion without craniectomy in rats","volume":"20","author":"E Z Longa","year":"1989","journal-title":"Stroke"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s00011-021-01515-5","article-title":"Dexmedetomidine exerts cerebral protective effects against cerebral ischemic\ninjury by promoting the polarization of M2 microglia via the Nrf2\/HO-1\/NLRP3\npathway","volume":"71","author":"N Wang","year":"2022","journal-title":"Inflamm Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"34","DOI":"10.31083\/j.jin2302034","article-title":"Astragalus Polysaccharide Promotes Neuronal Injury Repair via the Notch1\/NF\u03baB\nSignaling Axis in the Ventroposterior Thalamic Nucleus in Rats with Focal\nCerebral Ischemia","volume":"23","author":"W Li","year":"2024","journal-title":"J Integr Neurosci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2682","DOI":"10.1161\/hs1101.098367","article-title":"Intravenous administration of human umbilical cord blood reduces behavioral\ndeficits after stroke in rats","volume":"32","author":"J Chen","year":"2001","journal-title":"Stroke"},{"key":"ref19","first-page":"9912325","article-title":"Electroacupuncture Regulates Endoplasmic Reticulum Stress and\nAmeliorates Neuronal Injury in Rats with Acute Ischemic\nStroke","volume":"2021","author":"Y M Zhang","year":"2021","journal-title":"Evid Based Complement Alternat Med"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/j.amjmed.2021.07.027","article-title":"Ischemic Stroke","volume":"134","author":"S K Feske","year":"2021","journal-title":"Am J Med"},{"key":"ref21","first-page":"34","article-title":"Animal models of 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Part two: modeling cerebral ischemia","volume":"4","author":"M Bacigaluppi","year":"2010","journal-title":"Open Neurol J"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1369\/0022155413492159","article-title":"Melatonin ameliorates injury and specific responses of ischemic striatal neurons\nin rats","volume":"61","author":"Y Ma","year":"2013","journal-title":"J Histochem Cytochem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"112836","DOI":"10.1016\/j.biopha.2022.112836","article-title":"Formononetin protects against inflammation associated with cerebral\nischemia-reperfusion injury in rats by targeting the JAK2\/STAT3 signaling\npathway","volume":"149","author":"L Yu","year":"2022","journal-title":"Biomed Pharmacother"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1007\/s11011-024-01420-w","article-title":"Astragalus polysaccharide treatment relieves cerebral ischemia-reperfusion\ninjury by promoting M2 polarization of 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RATS","volume":"14","author":"Y Chen","year":"2017","journal-title":"Afr J Tradit Complement Altern Med"},{"key":"ref31","first-page":"203433","article-title":"Cerebrovascular and Neurological Disorders: Protective Role of NRF2","author":"F Sivandzade","year":"2019","journal-title":"Int J Mol Sci"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"157904","DOI":"10.1016\/j.phymed.2026.157904","article-title":"Rosmarinic acid alleviates ischemic stroke by targeting BAG3 to modulate\nautophagy via the P62-Keap1-Nrf2 pathway","volume":"154","author":"C Liu","year":"2026","journal-title":"Phytomedicine"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"e70662","DOI":"10.1111\/cns.70662","article-title":"Tetrahydrocurcumin ameliorates cerebral ischemia-reperfusion injury and restores\nblood-brain barrier dysfunction by inhibiting ferroptosis","volume":"31","author":"S Zhang","year":"2025","journal-title":"CNS Neurosci Ther"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.bbamcr.2018.02.010","article-title":"Nrf2-Keap1 signaling in oxidative and reductive stress","volume":"1865","author":"I Bellezza","year":"2018","journal-title":"Biochim Biophys Acta Mol Cell Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1007\/s12035-016-9707-7","article-title":"Nrf2 Weaves an Elaborate Network of Neuroprotection Against Stroke","volume":"54","author":"S Jiang","year":"2017","journal-title":"Mol Neurobiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1007\/s11064-023-04095-4","article-title":"SIRT5 Regulates Ferroptosis through the Nrf2\/HO-1 signaling axis to participate\nin ischemia-reperfusion injury in ischemic stroke","volume":"49","author":"J Li","year":"2024","journal-title":"Neurochem Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"103406","DOI":"10.1016\/j.redox.2024.103406","article-title":"Isoliquiritigenin alleviates cerebral ischemia-reperfusion injury by reducing\noxidative stress and ameliorating mitochondrial dysfunction via activating the\nNrf2 pathway","volume":"77","author":"X Lan","year":"2024","journal-title":"Redox Biol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2152746","DOI":"10.1155\/2022\/2152746","article-title":"Geraniin protects against cerebral ischemia\/reperfusion injury\nby suppressing oxidative stress and neuronal apoptosis via regulation of the\nNrf2\/HO-1 pathway","volume":"2022","author":"Y Yang","year":"2022","journal-title":"Oxid Med Cell Longev"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.2147\/DDDT.S503424","article-title":"Buyang huanwu decoction alleviates ischemic stroke injury by inhibiting\nferroptosis via the Nrf2\/GPX4 pathway","volume":"19","author":"H Huang","year":"2025","journal-title":"Drug Des Devel Ther"}],"container-title":["Drug Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2898-0240.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:32:37Z","timestamp":1789770757000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2898-0240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,13]]},"references-count":39,"journal-issue":{"issue":"07","published-online":{"date-parts":[[2026,9,18]]},"published-print":{"date-parts":[[2026,9]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2898-0240","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["2194-9379","2194-9387"],"issn-type":[{"value":"2194-9379","type":"print"},{"value":"2194-9387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,13]]}}}