{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:34:58Z","timestamp":1786120498332,"version":"build-2736575974"},"reference-count":49,"publisher":"Georg Thieme Verlag KG","funder":[{"name":"Jagiellonian University in Krak\u00f3w Medical\n                        College","award":["N42\/DBS\/000427"],"award-info":[{"award-number":["N42\/DBS\/000427"]}]},{"name":"University of Information Technology and Management in\n                        Rzesz\u00f3w","award":["DS 503-07-01-03"],"award-info":[{"award-number":["DS 503-07-01-03"]}]},{"DOI":"10.13039\/501100010621","name":"Medical University of Lublin","doi-asserted-by":"crossref","award":["DS24"],"award-info":[{"award-number":["DS24"]}],"id":[{"id":"10.13039\/501100010621","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Achillea filipendulina, a polyphenol-rich species, was used to establish\n                        in vitro shoot cultures and to compare their phytochemical and\n                    biological properties with those of field-grown material. In vitro shoots\n                    cultivated on agar medium (AFiv) and in Plantform bioreactors (AFB) were\n                    extracted with ethanol, 50% ethanol, or water and evaluated alongside extracts\n                    from field-grown plants (AFH). HPLC-ESI-QTOF-MS\/MS profiling revealed 37\n                    phenolic constituents. At the same time, quantitative analysis by LC-MS\n                    confirmed that chlorogenic acid was the major compound, reaching the highest\n                    concentrations in AFH and AFB ethanol-based extracts. In contrast, AFiv samples\n                    contained lower but consistently detectable levels. Similarly,\n                    p-hydroxybenzoic acid, quercetin, and rutin were quantified above LOQ in\n                    nearly all samples, with their highest amounts in AFH EtOH and 50%\u2009EtOH extracts\n                    and the lowest levels in AFiv H2O extracts, demonstrating clear\n                    solvent- and biomass-dependent variation.<\/jats:p>\n                  <jats:p>AFH extracts showed the strongest antioxidant activity (IC50\n                    50%\u2009EtOH=30.1; TPC\u2009=\u20091.32\u2009mg\u2009GAE\/g DW), while AFiv and AFB displayed lower TPC\n                    (0.36\u200a\u2013\u200a0.38 and 0.19\u200a\u2013\u200a0.30\u2009mg\u2009GAE\/g DW) and weaker activity\n                    (IC50\u2009=\u2009254\u2009\u00b5g\/mL and \u2265\u20091000\u2009\u00b5g\/mL). In tyrosinase assays, AFiv\n                    extracts were the most potent, inhibiting murine tyrosinase by 56.8\u200a\u2013\u200a62.8% and\n                    human tyrosinase by 17.6\u200a\u2013\u200a29.9%, comparable to kojic acid, whereas AFH and AFB\n                    demonstrated weaker or selective inhibition.<\/jats:p>\n                  <jats:p>Cytotoxicity testing showed that AFH extracts reduced HaCaT viability by\n                    25\u200a\u2013\u200a38%, while AFiv and AFB were non-cytotoxic and exhibited reduced or no\n                    cytotoxicity toward melanoma cells. Moreover, technological validation of the\n                    most potent AFiv 50EtOH extract within model O\/W and W\/O emulsions demonstrated\n                    exceptional 30-day physicochemical stability and compatibility.<\/jats:p>","DOI":"10.1055\/a-2910-9128","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T23:21:37Z","timestamp":1783984897000},"source":"Crossref","is-referenced-by-count":0,"title":["Skin-lightening Metabolites in Achillea filipendulina:\n                    Comparative Phytochemical and Bioactivity Profiling of Extracts from Shoot\n                    Cultures and Field-grown Plants"],"prefix":"10.1055","author":[{"given":"Karolina","family":"Czech","sequence":"additional","affiliation":[{"name":"Department of Cosmetology, University of Information Technology and\n                    Management, Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Kozyra","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plants Garden, Medical\n                    University of Lublin, Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6351-4047","authenticated-orcid":false,"given":"Agnieszka","family":"Szopa","sequence":"additional","affiliation":[{"name":"Department of Medicinal Plant and Mushroom Biotechnology, Faculty of\n                    Pharmacy, Jagiellonian University, Medical College, Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wirginia","family":"Kukula-Koch","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plants Garden, Medical\n                    University of Lublin, Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Gawe\u0142-B\u0119ben","sequence":"additional","affiliation":[{"name":"Department of Cosmetology, University of Information Technology and\n                    Management, Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"7276","DOI":"10.1038\/s41467-024-51556-7","article-title":"Island biogeography of the megadiverse plant family Asteraceae","volume":"15","author":"L Roeble","year":"2024","journal-title":"Nat Commun"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"3009","DOI":"10.3390\/ijms22063009","article-title":"The plants of the asteraceae family as agents in the protection of human\n                    health","volume":"22","author":"A 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