{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:52:35Z","timestamp":1792785155102,"version":"4.5.0"},"reference-count":22,"publisher":"Georg Thieme Verlag KG","issue":"CP","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Landsteiner Foundation for Blood Transfusion Research","id":[{"id":"https:\/\/ror.org\/05emg8z25","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Studienstiftung des Deutschen Volkes","id":[{"id":"https:\/\/ror.org\/05xwwfy96","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TH Open"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Background In the autoimmune bleeding disorder childhood immune thrombocytopenia (ITP), antinuclear antibodies (ANA) have been suggested as potential biomarkers for predicting chronic ITP. Unfortunately, published evidence remains conflicting and testing is not currently recommended in clinical routine.<\/jats:p>\n                  <jats:p>Aim This study aimed to validate the prognostic association of ANA with chronic ITP in newly diagnosed childhood ITP.<\/jats:p>\n                  <jats:p>Methods Data from 176 children with newly diagnosed ITP and a platelet count \u226420 \u00d7 109\/L enrolled in the Treatment With or Without IVIg in Kids with ITP (TIKI) randomized controlled trial were available. ANA testing was performed on sera obtained within 72 h of diagnosis, before immunomodulatory treatment. Children were followed for 12 months to assess chronic ITP development, intravenous immunoglobulin (IVIg) treatment response, and spontaneous recovery.<\/jats:p>\n                  <jats:p>Results ANA were detected in 36\/176 (20%) children. Chronic ITP developed in 21\/176 children; 4\/36 (11%) of ANA-positive versus 17\/140 (12%) of ANA-negative patients (OR 0.90; 95% CI: 0.25\u20132.65). No differences were observed in platelet counts, spontaneous recovery, or IVIg responses when comparing children with and without ANA. ANA provided no additional prognostic value beyond the Childhood ITP Recovery Score, a clinical prediction rule for recovery from ITP. ANA were not associated with anti-platelet antibodies by platelet immunofluorescence (PIFT) or monoclonal antibody-immobilization of platelet antigens (MAIPA).<\/jats:p>\n                  <jats:p>Conclusion ANA testing at the time of diagnosis was not helpful to predict chronic ITP, IVIg treatment responses, or spontaneous recovery among children with newly diagnosed ITP and a platelet count \u226420 \u00d7 109\/L. Future studies may evaluate whether ANA testing may be of value when performed at a later point in time.<\/jats:p>","DOI":"10.1055\/a-2938-6571","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T23:04:01Z","timestamp":1788822241000},"source":"Crossref","is-referenced-by-count":0,"special_numbering":"S","title":["Prognostic Re-evaluation of Antinuclear Antibodies in Childhood Immune Thrombocytopenia"],"prefix":"10.1055","volume":"10","author":[{"given":"Zeinab","family":"Muse","sequence":"first","affiliation":[{"name":"Astrid Lindgren Children\u2019s Hospital, Karolinska University Hospital, Stockholm County, Sweden","place":["Stockholm"],"department":["Pediatric Coagulation"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5555-376X","authenticated-orcid":false,"given":"David E.","family":"Schmidt","sequence":"first","affiliation":[{"name":"Astrid Lindgren Children\u2019s Hospital, Karolinska University Hospital, Stockholm County, Sweden","place":["Stockholm"],"department":["Pediatric Coagulation"]},{"name":"Sanquin Blood Supply Foundation, Noord-Holland, Netherlands","place":["Amsterdam"],"department":["Department Research"]},{"name":"Amsterdam University Medical Center, University of Amsterdam, Netherlands","place":["Amsterdam"],"department":["Landsteiner Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja M.J.","family":"Heitink-Poll\u00e9","sequence":"additional","affiliation":[{"name":"Princess Maxima Center for Pediatric Oncology, Netherlands","place":["Utrecht"]},{"name":"University Medical Centre Utrecht, Netherlands","place":["Utrecht"],"department":["Department of Pediatric Hematology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leendert","family":"Porcelijn","sequence":"additional","affiliation":[{"name":"PurSang Diagnostics, Noord-Holland, Netherlands","place":["Amsterdam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyra A.","family":"Gelderman","sequence":"additional","affiliation":[{"name":"PurSang Diagnostics, Noord-Holland, Netherlands","place":["Amsterdam"]},{"name":"Erasmus Medical Center, ZH, Netherlands","place":["Rotterdam"],"department":["Department of Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marrie C.A.","family":"Bruin","sequence":"additional","affiliation":[{"name":"Princess Maxima Center for Pediatric Oncology, Netherlands","place":["Utrecht"]},{"name":"University Medical Centre Utrecht, Netherlands","place":["Utrecht"],"department":["Department of Pediatric Hematology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Ellen","family":"Van der Schoot","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Noord-Holland, Netherlands","place":["Amsterdam"],"department":["Department Research"]},{"name":"Amsterdam University Medical Center, University of Amsterdam, Netherlands","place":["Amsterdam"],"department":["Landsteiner Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gestur","family":"Vidarsson","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Noord-Holland, Netherlands","place":["Amsterdam"],"department":["Department Research"]},{"name":"Utrecht Institute for Pharmaceutical Sciences, Netherlands","place":["Utrecht"],"department":["Department of Biomolecular Mass Spectrometry and Proteomics"]},{"name":"Bijvoet Center for Biomolecular Research, Utrecht University, Netherlands","place":["Utrecht"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masja","family":"De Haas","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Noord-Holland, Netherlands","place":["Amsterdam"],"department":["Department Research"]},{"name":"Leiden University Medical Center, ZH, Netherlands","place":["Leiden"],"department":["Department of Hematology"]},{"name":"Sanquin, Netherlands","place":["Amsterdam"],"department":["Department of Medical Affairs"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rick","family":"Kapur","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Noord-Holland, Netherlands","place":["Amsterdam"],"department":["Department Research"]},{"name":"Amsterdam University Medical Center, University of Amsterdam, Netherlands","place":["Amsterdam"],"department":["Landsteiner Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"issue":"02","key":"ref1","doi-asserted-by":"crossref","first-page":"E16","DOI":"10.3390\/jcm6020016","article-title":"Pathogenesis and therapeutic mechanisms in immune thrombocytopenia (ITP)","volume":"6","author":"A Zufferey","year":"2017","journal-title":"J Clin Med"},{"issue":"06","key":"ref2","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1097\/MOH.0000000000000612","article-title":"An update on the pathophysiology of immune thrombocytopenia","volume":"27","author":"J W Semple","year":"2020","journal-title":"Curr Opin Hematol"},{"issue":"03","key":"ref3","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1067\/S0022-3476(03)00245-2","article-title":"NOPHO ITP Working Group. 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