{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:53:29Z","timestamp":1792644809555,"version":"4.5.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thorac cardiovasc Surg"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Background\u2003Existing large-animal, ex vivo, cardiac perfusion models are restricted in their ability to establish an ischemia\/reperfusion condition as seen in cardiac surgery or transplantation. Other working heart systems only challenge one ventricle or require a substantially larger priming volume. We describe a novel biventricular cardiac perfusion system with reduced priming volume.<\/jats:p>\n                  <jats:p>Methods\u2003Juvenile pig hearts were cardiopleged, explanted, and reperfused ex vivo after 150 minutes of cold ischemia. Autologous whole blood was used as perfusate (minimal priming volume 350 mL). After 15 minutes of Langendorff perfusion (LM), the system was switched into a biventricular working mode (WM) and studied for 3 hours.<\/jats:p>\n                  <jats:p>Results\u2003During reperfusion, complete unloading of both ventricles and constant-pressure coronary perfusion was achieved. During working mode perfusion, the preload and afterload pressure of both ventricles was controlled within the targeted physiologic range. Functional parameters such as left ventricular work index were reduced in ex vivo working mode (in vivo: 787\u2009\u00b1\u2009186 vs. 1\u2009h WM 498\u2009\u00b1\u200966\u2009mm Hg\u00b7mL\/g\u00b7min; p\u2009&lt;\u20090.01), but remained stable throughout the following study period (3\u2009h WM 517\u2009\u00b1\u2009103\u2009mm Hg\u00b7mL\/g\u00b7min; p\u2009=\u20090.63). Along with the elevated workload during WM, myocardial metabolism and oxygen consumption increased compared with LM (0.021\u2009\u00b1\u20090.08 vs. 0.06\u2009\u00b1\u20090.01 mL\/min\/g; 1\u2009h after reperfusion). Histologic examination of the myocardium revealed no structural damage.<\/jats:p>\n                  <jats:p>Conclusion\u2003In the ex vivo perfusion system, stable hemodynamic and metabolic conditions can be established for a period of 3 hours while functional and blood parameters are easily accessible. Moreover, because of the minimal priming volume, the novel ex vivo cardiac perfusion circuit allows for autologous perfusion, using the limited amount of blood available from the organ donating animal.<\/jats:p>","DOI":"10.1055\/s-0036-1580604","type":"journal-article","created":{"date-parts":[[2016,4,4]],"date-time":"2016-04-04T19:31:00Z","timestamp":1459798260000},"page":"071-082","source":"Crossref","is-referenced-by-count":15,"title":["Large-Animal Biventricular Working Heart Perfusion System with Low Priming Volume\u2014Comparison between in vivo and ex vivo Cardiac Function"],"prefix":"10.1055","volume":"66","author":[{"given":"Tanja","family":"Mayr","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology, Ludwig Maximilian University, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Jauch","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology, Ludwig Maximilian University, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Guethoff","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Ludwig Maximilian University, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Buchholz","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Ludwig Maximilian University, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Reichart","sequence":"first","affiliation":[{"name":"Walter Brendel Center, Ludwig Maximilian University, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Bauer","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology, Ludwig Maximilian University, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan-Michael","family":"Abicht","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Ludwig Maximilian University, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2016,4,4]]},"container-title":["The Thoracic and Cardiovascular Surgeon"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0036-1580604.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T18:36:59Z","timestamp":1515523019000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0036-1580604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,4]]},"references-count":0,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2018,1,9]]},"published-print":{"date-parts":[[2018,1]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0036-1580604","relation":{},"ISSN":["0171-6425","1439-1902"],"issn-type":[{"value":"0171-6425","type":"print"},{"value":"1439-1902","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,4]]}}}