{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:56:59Z","timestamp":1790877419178,"version":"4.1.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"03","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Pediatr Surg"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:p>\n            Purpose\u2003The reconstruction of congenital diaphragmatic hernia or other congenital soft tissue defects often requires implants. These can be either degradable or permanent, each having their advantages. Whatever type is being used, the host response induced by implants plays a crucial role to determine the outcome. Macrophages are pivotal during implant remodeling; they are plastic and acquire in response to environmental stimuli either an inflammatory status and mediate subsequent fibrosis or a regulatory status and facilitate functional remodeling. Matrices engineered with mesenchymal stem cells (MSCs) have the capacity to modulate the host immune reaction. MSCs are believed to promote constructive remodeling of the implant through a regulatory macrophage response among others. Herein, we evaluate this potential of MSC derived from the amniotic fluid (AF-MSC), an interesting MSC type for neonatal reconstruction, on electrospun polylactic acid (PLA) scaffolds.<\/jats:p><jats:p>\n            Methods\u2003We seeded AF-MSC at a density of 1.105\/cm2 on electrospun PLA matrices and determined cell viability. In vivo, we used cell-seeded or cell-free PLA matrices for subcutaneous implantation in immune competent rats. The host immune response was evaluated by histomorphometry at 14 days postoperatively.<\/jats:p><jats:p>\n            Results\u2003The PLA matrix supported adherence and proliferation of AF-MSC. Fourteen days after implantation, PLA matrices were well penetrated by inflammatory cells, new blood vessels, and collagen fibers. AF-MSC\u2013seeded scaffolds were associated with a similar response yet with a decreased number of eosinophils, increased matrix degradation and collagen fiber deposition compared with controls. The amount of total macrophages and of M2-subtype was similar for all animals.<\/jats:p><jats:p>\n            Conclusion\u2003Electrospun PLA matrices are a suitable substrate for short-term culture of AF-MSC. In rats, addition of AF-MSC to PLA matrices modulates the host response after subcutaneous implantation, yet without a difference in macrophage profile compared with control.<\/jats:p>","DOI":"10.1055\/s-0037-1603522","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T18:55:58Z","timestamp":1496256958000},"page":"285-292","source":"Crossref","is-referenced-by-count":8,"title":["Modulation of the Early Host Response to Electrospun Polylactic Acid Matrices by Mesenchymal Stem Cells from the Amniotic Fluid"],"prefix":"10.1055","volume":"28","author":[{"given":"Flore","family":"Lesage","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabiniano","family":"Roman","sequence":"first","affiliation":[{"name":"Department of Material Science and Engineering, University of Sheffield Kroto Research Institute and Centre for Nanoscience and Technology, Sheffield, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savitree","family":"Pranpanus","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Prince of Songkla University, Songkla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Ospitalieri","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Zia","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Jimenez","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium"},{"name":"Department of Gynaecology and Obstetrics Cl\u00ednica Alemana, Facultad de Medicina Cl\u00ednica Alemana-Universidad Del Desarrollo, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila","family":"MacNeil","sequence":"first","affiliation":[{"name":"Department of Material Science and Engineering, University of Sheffield Kroto Research Institute and Centre for Nanoscience and Technology, Sheffield, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaan","family":"Toelen","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium"},{"name":"Department of Pediatrics, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Deprest","sequence":"additional","affiliation":[{"name":"Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium"},{"name":"Department of Obstetrics and Gynecology, University Hospitals Leuven, Leuven, Belgium"},{"name":"Department of Maternal Fetal Medicine, University College London Institute for Women's Health, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2017,5,31]]},"container-title":["European Journal of Pediatric Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0037-1603522.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T18:36:15Z","timestamp":1525804575000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0037-1603522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,31]]},"references-count":0,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2018,5,8]]},"published-print":{"date-parts":[[2018,6]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0037-1603522","relation":{},"ISSN":["0939-7248","1439-359X"],"issn-type":[{"value":"0939-7248","type":"print"},{"value":"1439-359X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,31]]}}}