{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:14:54Z","timestamp":1787004894832,"version":"build-2736575974"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"03","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Pediatr Surg"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:p>Background\u2003Modified multivisceral transplantation (MMVTx) refers to the use of a graft that includes all abdominal organs except the liver. The use of this type of transplant in children and adults expanded over the last years with good results. However, long-term survival in experimental models has not been reported. Our aim is to describe in detail some technical modifications of MMVTx to obtain long-term survival.<\/jats:p>\n                  <jats:p>Materials and Methods\u2003Syngeneic (Lewis\u2013Lewis) heterotopic MMVTx was performed in 16 male rats (180\u2013250 g). All procedures were performed under isoflurane anesthesia. The graft consisted of stomach, duodenopancreatic axis, spleen, and small bowel. The vascular pedicle consisted of a conduit of aorta, including the celiac trunk and the superior mesenteric artery (SMA), and the portal vein (PV). The engraftment was performed by end-to-side anastomosis to the infra-renal cava vein and aorta. After reperfusion, the graft was accommodated in the right side of the abdomen, and a terminal ileostomy performed. The native spleen was removed.<\/jats:p>\n                  <jats:p>Results\u2003Donor and recipient time was 39\u2009\u00b1\u20094.4 minutes and 69\u2009\u00b1\u20097 minutes, respectively; venous and arterial anastomosis time was 14\u2009\u00b1\u20091 minutes and 12.3\u2009\u00b1\u20091 minutes, respectively. Total ischemia time was 77.2\u2009\u00b1\u20097.9 minutes. Survival was 75% (12\/16), six were sacrificed after 2 hours, and six were kept alive for long-term evaluation (more than 1 week).<\/jats:p>\n                  <jats:p>Conclusion\u2003Long-term survival is reported after heterotopic MMVTx in rats. The heterotopic MMVTx with native spleen removal would potentially improve the existent models for transplant research. The usefulness of this model warrants further confirmation in allogeneic experiments.<\/jats:p>","DOI":"10.1055\/s-0038-1632375","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T18:43:30Z","timestamp":1519411410000},"page":"253-259","source":"Crossref","is-referenced-by-count":6,"title":["Modified Multivisceral Transplantation with Native Spleen Removal in Rats"],"prefix":"10.1055","volume":"29","author":[{"given":"Pablo","family":"Stringa","sequence":"additional","affiliation":[{"name":"Cirug\u00eda Experimental, Fundacion Investigacion Biomedica Del Hospital Universitario La Paz, Madrid, Spain"},{"name":"Instituto de Medicina Traslacional, Trasplante y Bioingenier\u00eda (IMETTYB), Universidad Favaloro-CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidia","family":"Arreola","sequence":"additional","affiliation":[{"name":"Hospital de Pediatr\u00eda, Centro M\u00e9dico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ane","family":"Moreno","sequence":"additional","affiliation":[{"name":"Cirug\u00eda Pedi\u00e1trica, Hospital Universitario La Paz, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlota","family":"Largo","sequence":"additional","affiliation":[{"name":"Cirug\u00eda Experimental, Hospital Universitario La Paz, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00edn","family":"Rumbo","sequence":"additional","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP-CONICET-UNLP), Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Hernandez","sequence":"additional","affiliation":[{"name":"Cirug\u00eda Experimental, Fundacion Investigacion Biomedica Del Hospital Universitario La Paz, Madrid, Spain"},{"name":"Cirug\u00eda Pedi\u00e1trica, Hospital Universitario La Paz, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2018,2,23]]},"container-title":["European Journal of Pediatric Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0038-1632375.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T18:39:51Z","timestamp":1557340791000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0038-1632375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,23]]},"references-count":0,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2019,5,8]]},"published-print":{"date-parts":[[2019,6]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0038-1632375","relation":{},"ISSN":["0939-7248","1439-359X"],"issn-type":[{"value":"0939-7248","type":"print"},{"value":"1439-359X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,23]]}}}