{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T03:53:42Z","timestamp":1768276422585,"version":"3.49.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>\n            Objective\u2003Activation of thromboxane A2 synthase (TXAS)\/thromboxane A2 (TXA2)\/thromboxane prostanoid (TP) receptor leads to arterial constriction, platelet aggregation and vascular injury. We attempted to characterize the microvascular dysfunction in ischaemia\/reperfusion injury using genetically modified TXAS\u2212\/\u2212, TP\u2212\/\u2212 and TXAS\u2212\/\u2212TP\u2212\/\u2212 mice.<\/jats:p><jats:p>\n            Approach and Results\u2003The cardiac micro-circulation and electrocardiograms were evaluated from B6, TXAS\u2212\/\u2212, TP\u2212\/\u2212 and TXAS\u2212\/\u2212TP\u2212\/\u2212 mice in response to intravenous saline, endothelin-1, U46619 (a TXA2 agonist) and myocardial ischaemia\/reperfusion injury. Cardiac function was investigated with myocardial permeability, the troponin I concentration and the infarct size. Myocardial TXAS, TP, endothelial nitric oxide (NO) synthase (eNOS), nicotinamide adenine dinucleotide phosphate oxidase 4 (NOx4), 4-hydroxynonenal, interleukin (IL)-1\u03b2, cell apoptosis, coronary effluent thromboxane B2 (TXB2) and superoxide anions (O2\n            \u2212) and NO concentrations were measured. Mice mesenteric reactivity in response to various drugs was assessed by wire myography. In vivo fluorescent platelet adhesiveness to the mesenteric arterial endothelium after FeCl3 stimulation was examined. In B6 mice, ischaemia\/reperfusion significantly increased levels of ST-segment elevation, myocardial TXAS, TP, NOx4, IL-1\u03b2, apoptosis, coronary endothelin-1, TXB2, O2\n            \u2212 release and the infarct size, with concomitant decreases in eNOS, NO concentrations and cardiac micro-circulation. These effects were remarkably depressed in TXAS\u2212\/\u2212, TP\u2212\/\u2212 and TXAS\u2212\/\u2212TP\u2212\/\u2212 mice. Aspirin treatment or depletion of the TXAS, TP or TXAS\/TP gene significantly attenuated the exaggerated vascular reactivity by vasoconstrictors and vasodilators and efficiently reduced platelet adhesion to the mesenteric endothelium under FeCl3 stimulation.<\/jats:p><jats:p>\n            Conclusion\u2003Inhibiting TXAS\/TXA2\/TP signalling confers microvascular protection against oxidative injury in both cardiac and mesenteric arteries.<\/jats:p>","DOI":"10.1055\/s-0038-1672206","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T23:03:31Z","timestamp":1539126211000},"page":"1982-1996","source":"Crossref","is-referenced-by-count":21,"title":["Genetic Depletion of Thromboxane A2\/Thromboxane-Prostanoid Receptor Signalling Prevents Microvascular Dysfunction in Ischaemia\/Reperfusion Injury"],"prefix":"10.1055","volume":"118","author":[{"given":"Chih-Yao","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Life Science, National Taiwan Normal University, Taipei, Taiwan"},{"name":"Department of Cardiovascular Surgery, Taipei City Hospital RenAi Branch, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen-Yen","family":"Chien","sequence":"additional","affiliation":[{"name":"Mackay Junior College of Medicine, Nursing and Management, New Taipei City, Taiwan"},{"name":"Department of Surgery, Mackay Memorial Hospital and Mackay Medical College, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Yin","family":"Qiou","sequence":"additional","affiliation":[{"name":"Department of Life Science, National Taiwan Normal University, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Chang","sequence":"additional","affiliation":[{"name":"High School Division, Taipei American School, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I-Shing","family":"Yu","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Center, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Yuan","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine and Cardiovascular Center, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiang-Ting","family":"Chien","sequence":"additional","affiliation":[{"name":"Department of Life Science, National Taiwan Normal University, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2018,10,9]]},"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0038-1672206.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T21:11:14Z","timestamp":1557177074000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0038-1672206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,9]]},"references-count":0,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2018,10,24]]},"published-print":{"date-parts":[[2018,11]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0038-1672206","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,9]]}}}