{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:49:10Z","timestamp":1790844550060,"version":"4.1.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"03","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In obesity, platelets are described as hyperactive, mainly based on increased platelet size and presence of pro-thrombotic plasmatic molecules. We explored platelet functions, including calcium signalling in obesity, and the effect of weight loss. We included 40 obese patients (women with body mass index [BMI] of \u2265 35 kg\/m2) who were to undergo gastric bypass surgery and 40 healthy lean subjects (women with BMI of\u2009&lt;\u200925 kg\/m2) as a control group. Approximately 1\u2009year after surgery, the obese patients lost weight (75% had a BMI\u2009&lt;\u200935 kg\/m2). They were explored a second time with the same healthy control for the same platelet experiments. Compared with controls, obese patients' platelets displayed reduced sensitivity to thrombin (aggregation EC50 increased by 1.9\u2009\u00b1\u20090.3-fold, p\u2009=\u20090.005) and a lower Ca2+ response (70\u2009\u00b1\u20097% decrease, p\u2009&lt;\u200910\u22124). In 17 pairs of patients, we performed additional experiments: in obese patients' platelets, thrombin-induced \u03b1IIb\u03b23 activation was significantly lower (p\u2009=\u20090.003) and sarco-endoplasmic reticulum Ca2+ATPase (SERCA3) expression was decreased (48\u2009\u00b1\u20096% decrease, p\u2009&lt;\u200910\u22124). These differences were abolished after weight loss. Interestingly, pharmacological inhibition of SERCA3 activity in control group's platelets mimicked similar alterations than in obese patients' platelets and was associated with defective adenosine diphosphate (ADP) secretion. Addition of ADP to agonist restored platelet functions in obese patients and in SERCA3-inhibited control platelets (five experiments) confirming the direct involvement of the SERCA3-dependent ADP secretion pathway. This is the first study demonstrating that platelets from obese patients are hypo-reactive, due to a deficiency of SERCA3-dependent ADP secretion. Weight loss restores SERCA3 activity and subsequent calcium signalling, \u03b1IIb\u03b23 activation, platelet aggregation and ADP secretion.<\/jats:p>","DOI":"10.1055\/s-0038-1677033","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T05:46:17Z","timestamp":1547790377000},"page":"384-396","source":"Crossref","is-referenced-by-count":18,"title":["Platelet Functions are Decreased in Obesity and Restored after Weight Loss: Evidence for a Role of the SERCA3-Dependent ADP Secretion Pathway"],"prefix":"10.1055","volume":"119","author":[{"given":"Ziane","family":"Ela\u00efb","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Lopez","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"},{"name":"Departamento de Fisiolog\u00eda, Catedr\u00e1tico de Universidad, Universidad de Extremadura, C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel","family":"Coupaye","sequence":"additional","affiliation":[{"name":"Service des Explorations Fonctionnelles, H\u00f4pital Louis Mourier, Assistance Publique\u2013H\u00f4pitaux de Paris, Colombes, France"},{"name":"Centre Int\u00e9gr\u00e9 Nord Francilien de prise en charge de l'Ob\u00e9sit\u00e9 (CINFO), Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Zuber","sequence":"additional","affiliation":[{"name":"Service de Recherche Clinique, Fondation Ophtalmologique A. de Rothschild, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Becker","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Kondratieff","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Rep\u00e9rant","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"P\u00e9pin","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Salomon","sequence":"additional","affiliation":[{"name":"Service de Recherche Clinique, Fondation Ophtalmologique A. de Rothschild, Paris, France"},{"name":"D\u00e9partement de Sant\u00e9 Publique, H\u00f4pital Louis Mourier, Assistance Publique\u2013H\u00f4pitaux de Paris, Colombes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"France","family":"Teillet","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie et Transfusion, H\u00f4pital Louis Mourier, Assistance Publique\u2013H\u00f4pitaux de Paris, Colombes, France"},{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Msika","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service de Chirurgie Digestive, H\u00f4pital Louis Mourier, Assistance Publique\u2013H\u00f4pitaux de Paris, Colombes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Denis","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"de Prost","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie et Transfusion, H\u00f4pital Louis Mourier, Assistance Publique\u2013H\u00f4pitaux de Paris, Colombes, France"},{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Rosa","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9gis","family":"Bobe","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 Mixte de Recherche\u2013Sant\u00e9 1176, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"St\u00e9panian","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"Service d'H\u00e9matologie Biologique, H\u00f4pital Lariboisi\u00e8re, Assistance Publique\u2013H\u00f4pitaux de Paris, Paris, France"},{"name":"EA3515 Institut Universitaire d'H\u00e9matologie, H\u00f4pital Saint Louis, Paris-Diderot, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2019,1,16]]},"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0038-1677033.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T17:20:42Z","timestamp":1557163242000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0038-1677033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,16]]},"references-count":0,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2019,2,25]]},"published-print":{"date-parts":[[2019,3]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0038-1677033","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,16]]}}}