{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T15:55:26Z","timestamp":1768146926828,"version":"3.49.0"},"reference-count":37,"publisher":"Federa\u00e7\u00e3o das Associa\u00e7\u00f5es de Ginecologia e Obstetr\u00edcia","issue":"09","license":[{"start":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T00:00:00Z","timestamp":1569196800000},"content-version":"vor","delay-in-days":22,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Bras Ginecol Obstet"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003To describe a population of pregnant women diagnosed with toxoplasmosis and their respective newborns, describing the hospital protocol for treatment and follow-up.<\/jats:p><jats:p>\n          Methods\u2003Retrospective cohort of pregnant women with acute toxoplasmosis infection and risk of transplacental transmission who were sent to the Fetal Medicine Group of Hospital de Cl\u00ednicas de Porto Alegre (HCPA) between - January 1, 2006 and December 31, 2016. All patients with confirmed disease were included. The diagnostic protocol and treatment were applied; a polymerase chain reaction (PCR) analysis of the amniotic fluid was used to diagnose toxoplasmosis and determine the treatment. The newborns were followed up at the pediatric outpatient clinic specializing in congenital infection. The patients who were not followed up or were not born in the HCPA were excluded.<\/jats:p><jats:p>\n          Results\u2003A total of 65 patients were confirmed to have gestational toxoplasmosis; 40 performed amniocentesis, and 6 (15%) were identified as having positive PCR in the amniotic fluid. In five of those cases, this result associated with the gestational age defined the triple therapy during pregnancy, and in one case, it defined the monotherapy (advanced gestational age). A total of 4 of these newborns were treated from birth with triple therapy for 10 months, 1 was not treated (due to maternal refusal), and 1 progressed to death within the first 54\u2009hours of life due to complications of congenital toxoplasmosis. Of the 34 remaining cases with a negative PCR, 33 were treated with monotherapy and 1 was treated with triple therapy (ultrasound findings); of these children, 9 (26.5%) presented negative immunoglobulin G (IgG), 24 (70.6%) presented positive IgG (but none presented positive immunoglobulin M [IgM]), and 1 (2,9%) presented alterations compatible with congenital disease and started treatment with the triple therapy soon after birth. Out of the total sample of 60 patients, among the 25 who did not perform amniotic fluid PCR, 5 were treated with triple therapy (ultrasound findings\/prior treatment) and 20 patients were submitted to monotherapy; only two newborns underwent treatment for congenital toxoplasmosis. Among the 65 cases of gestational toxoplasmosis, 6 (9,2%) children had a diagnosis of congenital toxoplasmosis, and 2 patients with triple therapy felt severe adverse effects of the medications.<\/jats:p><jats:p>\n          Conclusions\u2003The present study suggests that research on PCR screening of the amniotic fluid may be useful to identify patients with a higher potential for fetal complications, who may benefit from the poly-antimicrobial treatment. Patients with negative PCR results must continue to prevent fetal infection with monotherapy, without risk of fetal or maternal impairment.<\/jats:p>","DOI":"10.1055\/s-0039-1697034","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T22:31:23Z","timestamp":1569277883000},"page":"539-547","source":"Crossref","is-referenced-by-count":14,"title":["Follow-up of Toxoplasmosis during Pregnancy: Ten-Year Experience in a University Hospital in Southern Brazil"],"prefix":"10.61622","volume":"41","author":[{"given":"Amanda Andrade","family":"Diesel","sequence":"additional","affiliation":[{"name":"Fetal Medicine Group, Hospital de Cl\u00ednicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzana de Azevedo","family":"Zachia","sequence":"additional","affiliation":[{"name":"Fetal Medicine Group, Hospital de Cl\u00ednicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1214-5664","authenticated-orcid":false,"given":"Ana L\u00facia Letti","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Fetal Medicine Group, Hospital de Cl\u00ednicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Vilaverde","family":"Perez","sequence":"additional","affiliation":[{"name":"Fetal Medicine Group, Hospital de Cl\u00ednicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavio Antonio de Freitas","family":"Uberti","sequence":"additional","affiliation":[{"name":"Pediatrics Service, Hospital de Cl\u00ednicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Ant\u00f4nio de Azevedo","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Fetal Medicine Group, Hospital de Cl\u00ednicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"47794","published-online":{"date-parts":[[2019,9,23]]},"reference":[{"issue":"03","key":"ref1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1590\/0037-8682-0233-2014","article-title":"Toxoplasma gondii seropositivity and risk factors in pregnant women followed up by the Family Health Strategy","volume":"48","author":"R M de Quadros","year":"2015","journal-title":"Rev Soc Bras Med Trop"},{"issue":"01","key":"ref2","first-page":"59","article-title":"[Cross-sectional study on toxoplasmosis among female students on a university course in the Presidente Prudente region, State of S\u00e3o Paulo]","volume":"43","author":"CdeO Souza","year":"2010","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1590\/S0103-05822011000300006","article-title":"Abordagem diagn\u00f3stica e terap\u00eautica da toxoplasmose em gestantes e as repercuss\u00f5es no rec\u00e9m-nascido","volume":"29","author":"T M Pessanha","year":"2011","journal-title":"Rev Paul Pediatr"},{"key":"ref4","volume-title":"Aten\u00e7\u00e3o \u00e0 Sa\u00fade do Rec\u00e9m-Nascido: Guia para os Profissionais de Sa\u00fade. 2a ed","year":"2014"},{"issue":"11","key":"ref5","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1055\/s-0036-1593970","article-title":"Evaluation of pregnant and postpartum women's knowledge about toxoplasmosis in Rio Grande \u2013 RS, Brazil","volume":"38","author":"L M Lehmann","year":"2016","journal-title":"Rev Bras Ginecol Obstet"},{"issue":"03","key":"ref6","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1590\/S0036-46652003000300006","article-title":"Incidence of congenital toxoplasmosis in southern Brazil: a prospective study","volume":"45","author":"L Mozzatto","year":"2003","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"03","key":"ref7","doi-asserted-by":"crossref","first-page":"281","DOI":"10.4103\/0974-777X.83536","article-title":"Toxoplasmosis: a global threat","volume":"3","author":"J M Furtado","year":"2011","journal-title":"J Glob Infect Dis"},{"issue":"07","key":"ref8","doi-asserted-by":"crossref","first-page":"e0132719","DOI":"10.1371\/journal.pone.0132719","article-title":"Prematurity and low birth weight did not correlate with anti-toxoplasma gondii maternal serum profiles - 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