{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:35:59Z","timestamp":1773966959861,"version":"3.50.1"},"reference-count":0,"publisher":"Scientific Scholar","issue":"03","license":[{"start":{"date-parts":[[2019,10,7]],"date-time":"2019-10-07T00:00:00Z","timestamp":1570406400000},"content-version":"vor","delay-in-days":98,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurosciences in Rural Practice"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\nBackground\u2003Spinal cord injury (SCI) leads to serious complications involving primary trauma and progressive loss due to inflammation, local ischemia, or infection. Despite a worldwide annual incidence of 15 to 40 cases per million, methylprednisolone is the only treatment available to alleviate neurologic dysfunction; therefore, research is currently focused on identifying novel targets by biochemical and molecular studies.<\/jats:p><jats:p>\nPurpose\u2003Here, we investigated the expression of various molecular markers at the messenger ribonucleic acid (mRNA) and protein level at day 0 and day 30 post-SCI.<\/jats:p><jats:p>\nMethods\u2003Enzyme-linked immunosorbent assay (ELISA) was performed to determine the expression of CASPASE-3 and heat shock protein-27 (HSP-27) in serum samples. Real-time polymerase chain reaction (RT-PCR) was performed to determine the level of mRNA expression of vascular endothelial growth factor receptor-1 (VEGFR-1), VEGFR-2, HSP-27, monocyte chemoattractant protein-1 (MCP-1), and CASPASE-3.<\/jats:p><jats:p>\nResults\u2003HSP-27 expression at day 30, as compared with day 0, showed significant downregulation. In contrast, there was elevated expression of MCP-1. ELISA analysis showed no significant change in the expression of CASPASE-3 or HSP-27.<\/jats:p><jats:p>\nConclusion\u2003There may be possible opposing role of HSP-27 and MCP-1 governing SCI. Their association can be studied by designing in vitro studies.<\/jats:p>","DOI":"10.1055\/s-0039-1697683","type":"journal-article","created":{"date-parts":[[2019,10,7]],"date-time":"2019-10-07T22:56:29Z","timestamp":1570488989000},"page":"452-458","source":"Crossref","is-referenced-by-count":2,"title":["Altered Expression of Heat Shock Protein-27 and Monocyte Chemoattractant Protein-1 after Acute Spinal Cord Injury: A Pilot Study"],"prefix":"10.25259","volume":"10","author":[{"given":"Vidyasagar","family":"Boraiah","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shweta","family":"Modgil","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neuroscience Research Laboratory, Post Graduate Institute of Medical Education and Research, Chandigarh, India"},{"name":"Department of Zoology, Panjab University, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaushal","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neuroscience Research Laboratory, Post Graduate Institute of Medical Education and Research, Chandigarh, India"},{"name":"Centre for System Biology and Bioinformatics, Panjab University, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivek","family":"Podder","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Kamineni Institute of Medical Sciences, Narketpally, Telangana, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhava Sai","family":"Sivapuram","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Dr. Pinnamaneni Siddhartha Institute of Medical Sciences and Research Foundation, Chinna Avutapalli, Andhra Pradesh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gurwattan S.","family":"Miranpuri","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akshay","family":"Anand","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neuroscience Research Laboratory, Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay","family":"Goni","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2019,10,7]]},"container-title":["Journal of Neurosciences in Rural Practice"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0039-1697683.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,5]],"date-time":"2023-08-05T04:38:57Z","timestamp":1691210337000},"score":1,"resource":{"primary":{"URL":"https:\/\/ruralneuropractice.com\/altered-expression-of-heat-shock-protein-27-and-monocyte-chemoattractant-protein-1-after-acute-spinal-cord-injury-a-pilot-study\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":0,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2019,10,7]]},"published-print":{"date-parts":[[2019,7]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0039-1697683","relation":{},"ISSN":["0976-3147","0976-3155"],"issn-type":[{"value":"0976-3147","type":"print"},{"value":"0976-3155","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7]]}}}