{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T22:17:30Z","timestamp":1759011450490},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Pediatr Surg"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Introduction\u2003Bone morphogenetic proteins (BMPs) are a family of proteins that regulate proliferation and differentiation of intestinal epithelial cells. The purpose of this study was to evaluate the role of BMP signaling following intestinal ischemia\u2013reperfusion (IR) in a rat model.<\/jats:p><jats:p>\n          Materials and Methods\u2003Male Sprague-Dawley rats were divided into four experimental groups: Sham-24 and Sham-48 rats underwent laparotomy and were sacrificed 24 or 48\u2009hours later, respectively; IR-24 and IR-48 rats underwent occlusion of superior mesenteric artery and portal vein for 30\u2009minutes followed by 24 or 48\u2009hours of reperfusion, respectively. Enterocyte proliferation and apoptosis were determined at sacrifice. BMP-related genes and protein expression were determined using real-time polymerase chain reaction, Western blot, and immunohistochemistry for 48\u2009hours followed by IR.<\/jats:p><jats:p>\n          Results\u2003IR rats demonstrated a significant increase in BMP2 (twofold increase, p\u2009&lt;\u20090.05), BMP4 (sevenfold increase), STAT3 (70% increase), BMPR1 (70% increase) messenger ribonucleic acid levels in jejunum and was accompanied by a significant increase in BMP2 and BMP4 protein levels in jejunum (sixfold increase) (Western blot) and upward increase in the number of BMP-positive cells (by immunohistochemistry) in jejunal (48% increase) and ileal (56% increase) villi compared with Sham-48 animals. Elevation in BMP2 and BMP4 levels was associated with increased rates of cell proliferation and increased cell apoptosis.<\/jats:p><jats:p>\n          Conclusion\u2003Forty-eight hours following intestinal IR in rats, BMP signaling pathway was stimulated. The increase in BMP signaling pathway activity correlates with accelerated cell turnover.<\/jats:p>","DOI":"10.1055\/s-0039-1700550","type":"journal-article","created":{"date-parts":[[2019,11,10]],"date-time":"2019-11-10T23:31:51Z","timestamp":1573428711000},"page":"064-070","source":"Crossref","is-referenced-by-count":4,"title":["Accelerated Intestinal Epithelial Cell Turnover Correlates with Stimulated BMP Signaling Cascade following Intestinal Ischemia\u2013Reperfusion in a Rat"],"prefix":"10.1055","volume":"30","author":[{"given":"Yoav","family":"Ben-Shahar","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Adaptation and Recovery, Technion Israel Institute of Technology Ruth and Bruce Rappaport Faculty of Medicine, Haifa, Haifa, Israel"},{"name":"Department of Pediatric Surgery, Dana-Dwek Children's Hospital, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zaid","family":"Abassi","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Adaptation and Recovery, Technion Israel Institute of Technology Ruth and Bruce Rappaport Faculty of Medicine, Haifa, Haifa, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hila","family":"Kreizman Shefer","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Adaptation and Recovery, Technion Israel Institute of Technology Ruth and Bruce Rappaport Faculty of Medicine, Haifa, Haifa, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulia","family":"Pollak","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Adaptation and Recovery, Tel Aviv University Sackler Faculty of Medicine, Tel Aviv, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udayan","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Adaptation and Recovery, Technion Israel Institute of Technology Ruth and Bruce Rappaport Faculty of Medicine, Haifa, Haifa, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"Sukhotnik","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Dana-Dwek Children's Hospital, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel"},{"name":"Laboratory of Intestinal Adaptation and Recovery, Tel Aviv University Sackler Faculty of Medicine, Tel Aviv, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2019,11,10]]},"container-title":["European Journal of Pediatric Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0039-1700550.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T23:40:20Z","timestamp":1582069220000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0039-1700550"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,10]]},"references-count":0,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2020,2,18]]},"published-print":{"date-parts":[[2020,2]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0039-1700550","relation":{},"ISSN":["0939-7248","1439-359X"],"issn-type":[{"value":"0939-7248","type":"print"},{"value":"1439-359X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,10]]}}}