{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:44:05Z","timestamp":1773661445897,"version":"3.50.1"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"07","funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund FWF","doi-asserted-by":"crossref","award":["SFB54\u201304\/APF05404FW"],"award-info":[{"award-number":["SFB54\u201304\/APF05404FW"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteoprotegerin (OPG) regulates bone metabolism by reducing the activation of osteoclasts, but may also be involved in blood vessel calcification and atherosclerosis. Within endothelial cells OPG is stored in Weibel\u2013Palade bodies (WPBs). Blood kinetics of OPG are essentially unknown. We aimed to assess these using two distinct in vivo models; one after stimulation with desmopressin (DDAVP) and another after stimulation with lipopolysaccharide (LPS). Both clinical trials were conducted at the Department of Clinical Pharmacology at the Medical University of Vienna, Austria. Participants received desmopressin (0.3 \u00b5g\/kg), LPS (2\u2009ng\/kg), or placebo (sodium chloride 0.9%) with subsequent blood sampling at time points up to 24\u2009hours after administration. The primary objective of this study was to investigate the plasma kinetics of OPG after stimulation with desmopressin and LPS. Secondary analyses included the release of other WPB contents including von Willebrand factor (vWF). This analysis included 31 healthy volunteers (n\u2009=\u200916 for desmopressin and placebo, n\u2009=\u200915 for LPS). Infusion of desmopressin did not increase OPG concentrations compared with placebo, while LPS infusion significantly increased OPG levels, both compared with desmopressin (p\u2009&lt;\u20090.0001) and to placebo (p\u2009=\u20090.004), with a maximum of \u223ctwofold increase in OPG levels \u223c6\u2009hours after infusion. von Willebrand factor levels increased after both desmopressin and LPS infusion (p\u2009&lt;\u20090.0001), with a maximum of \u223cthreefold increase 2\u2009hours after desmopressin and a maximum of \u223ctwofold increase 6\u2009hours after LPS administration. In conclusion, we report that, in contrast to vWF, OPG is not released upon stimulation with desmopressin, but increases significantly during experimental endotoxemia.<\/jats:p>","DOI":"10.1055\/s-0040-1712448","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T06:45:26Z","timestamp":1591080326000},"page":"1108-1115","source":"Crossref","is-referenced-by-count":6,"title":["Differential Osteoprotegerin Kinetics after Stimulation with Desmopressin and Lipopolysaccharides In Vivo"],"prefix":"10.1055","volume":"120","author":[{"given":"Nina","family":"Buchtele","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria"},{"name":"Department of Medicine I, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarina D.","family":"Kovacevic","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1462-5397","authenticated-orcid":false,"given":"Christine","family":"Brostjan","sequence":"additional","affiliation":[{"name":"Department of Surgery, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Schwameis","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Hayden","sequence":"additional","affiliation":[{"name":"Department of Surgery, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulla","family":"Derhaschnig","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christa","family":"Firbas","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Jilma","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Schoergenhofer","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,5,29]]},"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0040-1712448.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T22:50:55Z","timestamp":1594075855000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0040-1712448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,29]]},"references-count":0,"journal-issue":{"issue":"07","published-online":{"date-parts":[[2020,7,6]]},"published-print":{"date-parts":[[2020,7]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0040-1712448","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,29]]}}}