{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T10:54:00Z","timestamp":1776768840801,"version":"3.51.2"},"reference-count":39,"publisher":"Georg Thieme Verlag KG","issue":"10","funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology of Taiwan","doi-asserted-by":"crossref","award":["105-2314-B-006-057-MY3"],"award-info":[{"award-number":["105-2314-B-006-057-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology of Taiwan","doi-asserted-by":"crossref","award":["108-2314-B-006-098-MY3"],"award-info":[{"award-number":["108-2314-B-006-098-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Higher Education Sprout Project, Ministry of Education to the Headquarters of University Advancement at National Cheng Kung University","award":["D108-G2512"],"award-info":[{"award-number":["D108-G2512"]}]},{"name":"Higher Education Sprout Project, Ministry of Education to the Headquarters of University Advancement at National Cheng Kung University","award":["D109-G2512"],"award-info":[{"award-number":["D109-G2512"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Background\u2003Arterial thrombosis is initiated by atherosclerotic plaque damage, prothrombotic material release and platelet aggregation. Platelets are primary mediators involved in thrombosis and cooperate with vascular and immune cells.<\/jats:p><jats:p>\n          Objective\u2003Herein, we investigated how activated platelets interacted with monocytes in atherothrombosis.<\/jats:p><jats:p>\n          Methods and Results\u2003We collected patients' blood from coronary arteries during percutaneous coronary intervention and measured platelet activity. Platelets from coronary arteries had higher pseudopodium expression and activity in patients with acute coronary syndrome (ACS). Ribosome profiling of platelets from coronary blood mapped a vigorous upregulation of Rho GTPases and their downstream effectors. RhoA activated downstream Rho-associated coiled-coil containing protein kinase (ROCK), and ROCK increased surface P-selectin in coronary blood platelets. The interaction between platelets and monocytes was observed in vitro, and was found in ruptured coronary plaques of ACS. Further we found that activated platelets promoted monocytes transmigration, which could be suppressed in the presence of ROCK inhibitors. The increased surface P-selectin on thrombin-induced platelets interacted with monocytes to upregulate monocyte chemokine receptor 2 (CCR2) expression via the ROCK pathway. The expression of CCR2 was higher in monocyte\u2013platelet aggregates than in monocytes without platelets. Finally, using the Asian Screening Array BeadChip, we identified single-nucleotide polymorphism (SNP) associated with cardiovascular events. Notably, patients having homozygous major alleles of the RHOA SNP rs11706370 presented with higher risks of cardiovascular events.<\/jats:p><jats:p>\n          Conclusion\u2003Through ROCK-activated cytoskeleton remodeling and P-selectin expression, platelets were recruited and interacted synergistically with high CCR2-expressing monocytes to induce thromboinflammation in atherothrombosis.<\/jats:p>","DOI":"10.1055\/s-0040-1714278","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T23:11:12Z","timestamp":1599088272000},"page":"1417-1431","source":"Crossref","is-referenced-by-count":23,"title":["The Role of ROCK in Platelet\u2013Monocyte Collaborative Induction of Thromboinflammation during Acute Coronary Syndrome"],"prefix":"10.1055","volume":"120","author":[{"given":"Ling-Wei","family":"Hsu","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"Division of Cardiology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Ting","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"Department of Cardiology, Chi Mei Medical Center, Tainan, Taiwan"},{"name":"Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Huang","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"Division of Cardiology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3643-5204","authenticated-orcid":false,"given":"Ping-Yen","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"Division of Cardiology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1161\/CIRCRESAHA.114.302721","article-title":"Mechanisms of plaque formation and rupture","volume":"114","author":"J F Bentzon","year":"2014","journal-title":"Circ Res"},{"issue":"11","key":"ref2","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1038\/nm1102-1227","article-title":"Platelets in atherothrombosis","volume":"8","author":"Z M Ruggeri","year":"2002","journal-title":"Nat Med"},{"issue":"S 01","key":"ref3","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1538-7836.2007.02517.x","article-title":"Platelets, inflammation and atherosclerosis","volume":"5","author":"S Lindemann","year":"2007","journal-title":"J Thromb 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Insights from tissue factor, monocyte-platelet aggregates and novel mechanisms","volume":"102","author":"E Shantsila","year":"2009","journal-title":"Thromb Haemost"},{"issue":"01","key":"ref38","doi-asserted-by":"crossref","first-page":"5149","DOI":"10.1038\/s41598-017-05601-9","article-title":"Activated monocytes enhance platelet-driven contraction of blood clots via tissue factor expression","volume":"7","author":"A D Peshkova","year":"2017","journal-title":"Sci Rep"},{"issue":"11","key":"ref39","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1161\/01.RES.0000149518.86865.3e","article-title":"Crucial role of the CCL2\/CCR2 axis in neointimal hyperplasia after arterial injury in hyperlipidemic mice involves early monocyte recruitment and CCL2 presentation on platelets","volume":"95","author":"A Schober","year":"2004","journal-title":"Circ Res"}],"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0040-1714278.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T01:56:13Z","timestamp":1600912573000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0040-1714278"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,2]]},"references-count":39,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,9,23]]},"published-print":{"date-parts":[[2020,10]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0040-1714278","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,2]]}}}