{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T15:41:46Z","timestamp":1793202106724,"version":"4.5.0"},"reference-count":15,"publisher":"Scientific Scholar","issue":"02","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":31,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\nBackground and Objective\u2003Serotonin levels are increased in acute appendicitis. We investigated the possible source of this increase. The aim of this study was to compare the distribution and density of epithelial and nonepithelial enterochromaffin (EC) cells as well as numbers of degranulated and nondegranulated mast cells in different layers of normal appendices and acute appendicitis.<\/jats:p><jats:p>\nMethods\u2003Sections from 15 cases of acute appendicitis and 10 cases where the appendix was morphologically normal were stained with Hematoxylin &amp; Eosin, Toluidine blue, and immunohistochemically for chromogranin and CD-117. EC cells stained by chromogranin were counted per crypt and extraepithelial EC cells counted and expressed as cells per unit area (mm2). Mast cells stained by Toluidine blue and CD-117 were counted in lamina propria, submucosa, and muscle layers. The difference between Toluidine blue and CD117 stained mast cells was taken to be an estimate of degranulated cells. The cell counts were expressed per unit area (mm2) as well as per cross-sectional area of the appendix.<\/jats:p><jats:p>\nResults\u2003There was no statistically significant difference in epithelial and extraepithelial EC cells between acute appendicitis and normal appendix. Estimated mast cell degranulation as indicated by mast cell counts per cross-sectional area is greatly increased in acute appendicitis when compared with normal.<\/jats:p><jats:p>\nConclusion\u2003Degranulated mast cells rather than EC cells may be the main source of raised serotonin in acute appendicitis.<\/jats:p>","DOI":"10.1055\/s-0040-1716476","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T22:51:34Z","timestamp":1599000694000},"page":"141-146","source":"Crossref","is-referenced-by-count":6,"title":["Enterochromaffin Cells and Mast Cells in Acute Appendicitis"],"prefix":"10.25259","volume":"12","author":[{"given":"Bhavya P.","family":"Mohan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Government Medical College, Thrissur, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.P.","family":"Aravindan","sequence":"additional","affiliation":[{"name":"ICMR Emeritus Scientist, Government Medical College, Calicut, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1136\/emermed-2015-205559","article-title":"Does elevated urinary 5-hydroxyindole acetic acid level predict acute appendicitis in children?","volume":"33","author":"A Bosak Versic","year":"2016","journal-title":"Emerg Med J"},{"key":"ref2","first-page":"437","article-title":"Acute appendicitis and plasma concentration of serotonin","volume":"153","author":"P R\u00f8rdam","year":"1987","journal-title":"Acta Chir Scand"},{"issue":"04","key":"ref3","first-page":"491","article-title":"Eosinophils in acute appendicitis: possible significance","volume":"40","author":"K P Aravindan","year":"1997","journal-title":"Indian J Pathol Microbiol"},{"issue":"02","key":"ref4","doi-asserted-by":"crossref","first-page":"172","DOI":"10.4103\/0377-4929.41642","article-title":"Evidence for eosinophil degranulation in acute appendicitis","volume":"51","author":"G Santosh","year":"2008","journal-title":"Indian J Pathol Microbiol"},{"issue":"04","key":"ref5","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.ajem.2008.03.016","article-title":"The importance of urine 5-hydroxyindoleacetic acid levels in the early diagnosis of acute appendicitis","volume":"27","author":"O Mentes","year":"2009","journal-title":"Am J Emerg Med"},{"issue":"11","key":"ref6","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/j.clinbiochem.2004.07.003","article-title":"Urinary 5-hydroxy indole acetic acid as a test for early diagnosis of acute appendicitis","volume":"37","author":"S Bolandparvaz","year":"2004","journal-title":"Clin Biochem"},{"issue":"01","key":"ref7","first-page":"18","article-title":"Evaluation of plasma serotonin concentration in acute appendicitis","volume":"16","author":"U Kalra","year":"1997","journal-title":"Indian J Gastroenterol"},{"issue":"01","key":"ref8","first-page":"40","article-title":"The value of 5-hydroxy indole acetic acid measurement in spot urine in diagnosis of acute appendicitis","volume":"81","author":"M T Oruc","year":"2004","journal-title":"East Afr Med J"},{"issue":"11","key":"ref9","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1111\/j.1600-0463.2008.00916.x","article-title":"Serotonin content of normal and inflamed appendix: a possible role of serotonin in acute appendicitis","volume":"116","author":"M Vasei","year":"2008","journal-title":"APMIS"},{"issue":"02","key":"ref10","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.jaci.2006.09.003","article-title":"Human mast cells are capable of serotonin synthesis and release","volume":"119","author":"N M Kushnir-Sukhov","year":"2007","journal-title":"J Allergy Clin Immunol"},{"issue":"05","key":"ref11","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s12262-008-0066-0","article-title":"Eosinophils, mast cells, nerves and ganglion cells in appendicitis","volume":"70","author":"U R Singh","year":"2008","journal-title":"Indian J Surg"},{"issue":"02","key":"ref12","first-page":"229","article-title":"Mast cells in surgically resected appendices","volume":"49","author":"V V Mysorekar","year":"2006","journal-title":"Indian J Pathol Microbiol"},{"issue":"06","key":"ref13","doi-asserted-by":"crossref","first-page":"3599","DOI":"10.4049\/jimmunol.173.6.3599","article-title":"Cutting edge: serotonin is a chemotactic factor for eosinophils and functions additively with eotaxin","volume":"173","author":"S A Boehme","year":"2004","journal-title":"J Immunol"},{"issue":"11","key":"ref14","first-page":"571","article-title":"Mast cell count in surgically resected appendices","volume":"95","author":"R Naik","year":"1997","journal-title":"J Indian Med Assoc"},{"issue":"01","key":"ref15","first-page":"54","article-title":"Mast cell density and neuronal hypertrophy in patients with acute appendicitis","volume":"14","author":"N Co\u015fkun","year":"2003","journal-title":"Turk J Gastroenterol"}],"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0040-1716476.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T08:16:46Z","timestamp":1703405806000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/enterochromaffin-cells-and-mast-cells-in-acute-appendicitis\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":15,"journal-issue":{"issue":"02","published-online":{"date-parts":[[2020,9,1]]},"published-print":{"date-parts":[[2020,8]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0040-1716476","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8]]}}}