{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T03:16:07Z","timestamp":1785208567762,"version":"3.55.0"},"reference-count":40,"publisher":"Federa\u00e7\u00e3o das Associa\u00e7\u00f5es de Ginecologia e Obstetr\u00edcia","issue":"02","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Bras Ginecol Obstet"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003To investigate whether follicular fluid (FF) from infertile women with mild endometriosis (ME) alters in vitro bovine embryo development, and whether the antioxidants N-acetyl-cysteine (NAC) and\/or L-carnitine (LC) could prevent such damages.<\/jats:p><jats:p>\n          Methods\u2003Follicular fluid was obtained from infertile women (11 with ME and 11 control). Bovine oocytes were matured in vitro divided in: No-FF, with 1% of FF from control women (CFF) or ME women (MEFF); with 1.5\u2009mM NAC (CFF\u2009+\u2009NAC, MEFF\u2009+\u2009NAC), with 0.6\u2009mg\/mL LC (CFF\u2009+\u2009LC, MEFF\u2009+\u2009LC), or both antioxidants (CFF\u2009+\u2009NAC\u2009+\u2009LC, MEFF\u2009+\u2009NAC\u2009+\u2009LC). After in vitro fertilization, in vitro embryo culture was performed for 9 days.<\/jats:p><jats:p>\n          Results\u2003A total of 883 presumptive zygotes were cultured in vitro. No differences were observed in cleavage rate (p\u2009=\u20090.5376) and blastocyst formation rate (p\u2009=\u20090.4249). However, the MEFF group (12.5%) had lower hatching rate than the No-FF (42.1%, p\u2009=\u20090.029) and CFF (42.9%, p\u2009=\u20090.036) groups. Addition of antioxidants in the group with CFF did not alter hatching rate (p \u2265 0.56), and in groups with MEFF, just NAC increased the hatching rate [(MEFF: 12.5% versus MEFF\u2009+\u2009NAC: 44.4% (p\u2009=\u20090.02); vs MEFF\u2009+\u2009LC: 18.8% (p\u2009=\u20090.79); versus MEFF\u2009+\u2009NAC\u2009+\u2009LC: 30.8% (p\u2009=\u20090.22)].<\/jats:p><jats:p>\n          Conclusion\u2003Therefore, FF from infertile women with ME added to medium of in vitro maturation of bovine oocytes impairs hatching rate, and NAC prevented these damages, suggesting involvement of oxidative stress in worst of oocyte and embryo quality of women with ME.<\/jats:p>","DOI":"10.1055\/s-0040-1718443","type":"journal-article","created":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T06:38:42Z","timestamp":1611902322000},"page":"119-125","source":"Crossref","is-referenced-by-count":15,"title":["Follicular Fluid from Infertile Women with Mild Endometriosis Impairs In Vitro Bovine Embryo Development: Potential Role of Oxidative Stress"],"prefix":"10.61622","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6760-283X","authenticated-orcid":false,"given":"Vanessa Silvestre Innocenti","family":"Giorgi","sequence":"additional","affiliation":[{"name":"Human Reproduction Division, Department of Gynecology and Obstetrics, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9308-8344","authenticated-orcid":false,"given":"Rui Alberto","family":"Ferriani","sequence":"additional","affiliation":[{"name":"Human Reproduction Division, Department of Gynecology and Obstetrics, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, SP, Brazil"},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Bras\u00edlia, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2368-4188","authenticated-orcid":false,"given":"Paula Andrea","family":"Navarro","sequence":"additional","affiliation":[{"name":"Human Reproduction Division, Department of Gynecology and Obstetrics, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, SP, Brazil"},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Bras\u00edlia, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"47794","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"issue":"03","key":"ref1","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1016\/j.fertnstert.2012.06.029","article-title":"Pathogenesis and pathophysiology of endometriosis","volume":"98","author":"R O Burney","year":"2012","journal-title":"Fertil 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