{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T05:30:44Z","timestamp":1792387844241,"version":"4.4.0"},"reference-count":10,"publisher":"Scientific Scholar","issue":"03","license":[{"start":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T00:00:00Z","timestamp":1606089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ongoing COVID-19 pandemic has hugely impacted the economy of many countries, and there is an acute shortage of diagnostic resources. With the exponential increase in the number of cases and necessity to screen large number of people, there is a steep increase in the demand for diagnostic kits. Pooled-sample testing is a promising strategy to screen a large population rapidly with limited resources. The aim of this work was to compile a cohesive literature review of the effectiveness and accuracy of pooled-sample testing in the detection of SARS-CoV-2 and critically analyze its limitations. Medline, Google Scholar, Embase, and preprint servers (e.g., bioRxiv) were searched for literature on pooled testing for diagnosis of COVID-19, and out of initial 60 articles\/reports, nine original articles were retained. Optimal pool size (number of samples in a pool) seemed to be dependent on factors like prevalence or rate of positivity in community. In low-prevalence localities pool size of around 30 seemed to be effective as observed by some authors. All the researchers had found significant reduction in number of tests (depending on pool size, stages, and pooling design), leading to conservation of resources. Pooling can be done with extracted RNA eluate or directly with patient\u2019s sample before extraction. This leads to further reduction in consumables, time and manpower. Risk of false negativity in samples with high-threshold cycle (i.e., low-viral load) value was a concern. Some researchers suggest adding few additional cycles to lower the chances of missing positive cases with low-Ct value. Lower limit of detection (LoD) of RT-PCR kits, that is, sensitivity of kits was another factor to consider. Thus, in a country like India, given the economic benefit and scarcity of resources, pooling strategy can be very effective, especially in low-prevalence areas and in low-risk contacts.<\/jats:p>","DOI":"10.1055\/s-0040-1721159","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T13:27:49Z","timestamp":1606224469000},"page":"212-218","source":"Crossref","is-referenced-by-count":34,"title":["Effectiveness of Sample Pooling Strategies for SARS-CoV-2 Mass Screening by RT-PCR: A Scoping Review"],"prefix":"10.25259","volume":"12","author":[{"given":"Sangeeta","family":"Deka","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Virbhadra Road, Rishikesh, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepjyoti","family":"Kalita","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Virbhadra Road, Rishikesh, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2020,11,23]]},"reference":[{"issue":"04","key":"ref1","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1038\/s41564-020-0695-z","article-title":"The species severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2","volume":"5","author":"Coronaviridae Study Group of the International Committee on Taxonomy of Viruses","year":"2020","journal-title":"Nat Microbiol"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.57309","article-title":"SARS-CoV-2 (COVID-19) by the numbers","author":"Y M Bar-On","year":"2020","journal-title":"eLife"},{"issue":"04","key":"ref6","doi-asserted-by":"crossref","first-page":"2000058","DOI":"10.2807\/1560-7917.ES.2020.25.4.2000058","article-title":"Pattern of early human-to-human transmission of Wuhan 2019 novel coronavirus (2019-nCoV), December 2019 to January 2020","volume":"25","author":"J Riou","year":"2020","journal-title":"Euro Surveill"},{"issue":"03","key":"ref7","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1128\/JCM.05631-11","article-title":"Pooling nasopharyngeal\/throat swab specimens to increase testing capacity for influenza viruses by PCR","volume":"50","author":"T T Van","year":"2012","journal-title":"J Clin Microbiol"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa531","article-title":"Evaluation of COVID-19 RT-qPCR test in multi-sample pools","author":"I Yelin","year":"2020","journal-title":"Clin Infect Dis"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30362-5","article-title":"Pooling of samples for testing for SARS-CoV-2 in asymptomatic people","author":"S Lohse","year":"2020","journal-title":"Lancet Infect Dis"},{"issue":"06","key":"ref12","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1093\/ajcp\/aqaa064","article-title":"Assessment of specimen pooling to conserve SARS CoV-2 testing resources","volume":"153","author":"B Abdalhamid","year":"2020","journal-title":"Am J Clin Pathol"},{"issue":"19","key":"ref13","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1001\/jama.2020.5445","article-title":"Sample Pooling as a Strategy to Detect Community Transmission of SARS-CoV-2","volume":"323","author":"C A Hogan","year":"2020","journal-title":"JAMA"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104382","article-title":"Multi-stage group testing improves efficiency of large-scale COVID-19 screening","author":"J N Eberhardt","year":"2020","journal-title":"J Clin Virol"},{"issue":"01","key":"ref19","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1186\/s12967-019-1992-2","article-title":"A methodology for deriving the sensitivity of pooled testing, based on viral load progression and pooling dilution","volume":"17","author":"N T Nguyen","year":"2019","journal-title":"J Transl Med"}],"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0040-1721159.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T08:16:52Z","timestamp":1703405812000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/effectiveness-of-sample-pooling-strategies-for-sars-cov-2-mass-screening-by-rt-pcr-a-scoping-review\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,23]]},"references-count":10,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2020,11,23]]},"published-print":{"date-parts":[[2020,12]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0040-1721159","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,23]]}}}