{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:33:49Z","timestamp":1792636429422,"version":"4.5.0"},"reference-count":53,"publisher":"Georg Thieme Verlag KG","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Perinatol"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:p>\n            Objective\u2003The study aimed to assess the feasibility of creating and transplanting human umbilical cord mesenchymal stem cell sheets applied to a rat model of hysterotomy, and additionally to determine benefits of human umbilical cord mesenchymal stem cell sheet transplantation in reducing uterine fibrosis and scarring.<\/jats:p><jats:p>\n            Study Design\u2003Human umbilical cord mesenchymal stem cell sheets are generated by culturing human umbilical cord mesenchymal stem cells on thermo-responsive cell culture plates. The temperature-sensitive property of these culture dishes facilitates normal cell culture in a thin contiguous layer and allows for reliable recovery of intact stem cell sheets without use of destructive proteolytic enzymes.<\/jats:p><jats:p>We developed a rat hysterotomy model using nude rats. The rat uterus has two distinct horns: one horn provided a control\/untreated scarring site, while the second horn was the cell sheet transplantation site.<\/jats:p><jats:p>On day 14 following surgery, complete uteri were harvested and subjected to histologic evaluations of all hysterotomy sites.<\/jats:p><jats:p>\n            Results\u2003The stem cell sheet culture process yielded human umbilical cord mesenchymal stem cell sheets with surface area of approximately 1\u2009cm2.<\/jats:p><jats:p>Mean myometrial thickness in the cell sheet-transplanted group was 274\u2009\u03bcm compared with 191\u2009\u03bcm in the control group (p\u2009=\u20090.02). Mean fibrotic surface area in the human umbilical cord mesenchymal stem cell sheet-transplanted group was 95,861\u2009\u03bcm2 compared with 129,185\u2009\u03bcm2 in the control group. Compared with control horn sites, cell sheet-transplanted horns exhibited significantly smaller fibrotic-to-normal myometrium ratios (0.18 vs. 0.27, respectively, p\u2009=\u20090.029). Mean number of fibroblasts in cell sheet-transplanted horns was significantly smaller than the control horns (483 vs. 716\/mm2, respectively, p\u2009=\u20090.001).<\/jats:p><jats:p>\n            Conclusion\u2003Human umbilical cord mesenchymal stem cell sheet transplantation is feasible in a rat model of hysterotomy. Furthermore, use of stem cell sheets reduces fibroblast infiltration and uterine scar fibrotic tissue formation during hysterotomy healing, potentially mitigating risks of uterine scar formation.<\/jats:p><jats:p>\n            Key Points\n          <\/jats:p>","DOI":"10.1055\/s-0040-1721718","type":"journal-article","created":{"date-parts":[[2020,12,27]],"date-time":"2020-12-27T23:39:05Z","timestamp":1609112345000},"page":"1212-1222","source":"Crossref","is-referenced-by-count":12,"title":["Human Mesenchymal Stem Cell Sheets Improve Uterine Incision Repair in a Rodent Hysterotomy Model"],"prefix":"10.1055","volume":"39","author":[{"given":"Goro","family":"Kuramoto","sequence":"additional","affiliation":[{"name":"Cell Sheet Tissue Engineering Center, Department of Pharmaceutics and Pharmaceutical Chemistry, Health Sciences, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7660-4968","authenticated-orcid":false,"given":"Ibrahim A.","family":"Hammad","sequence":"additional","affiliation":[{"name":"Intermountain Healthcare, Salt Lake City, Utah"},{"name":"Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Health Sciences, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett D.","family":"Einerson","sequence":"additional","affiliation":[{"name":"Intermountain Healthcare, Salt Lake City, Utah"},{"name":"Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Health Sciences, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda A.","family":"Allshouse","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Health Sciences, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Debbink","sequence":"additional","affiliation":[{"name":"Intermountain Healthcare, Salt Lake City, Utah"},{"name":"Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Health Sciences, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Grainger","sequence":"additional","affiliation":[{"name":"Cell Sheet Tissue Engineering Center, Department of Pharmaceutics and Pharmaceutical Chemistry, Health Sciences, University of Utah, Salt Lake City, Utah"},{"name":"Department of Biomedical Engineering, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Silver","sequence":"additional","affiliation":[{"name":"Intermountain Healthcare, Salt Lake City, Utah"},{"name":"Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Health Sciences, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruo","family":"Okano","sequence":"additional","affiliation":[{"name":"Cell Sheet Tissue Engineering Center, Department of Pharmaceutics and Pharmaceutical Chemistry, Health Sciences, University of Utah, Salt Lake City, Utah"},{"name":"Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2020,12,27]]},"reference":[{"issue":"29","key":"ref1","first-page":"1","article-title":"National Hospital Discharge Survey: 2007 summary","author":"M J Hall","year":"2010","journal-title":"Natl Health Stat Rep"},{"issue":"06","key":"ref2","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1097\/01.AOG.0000219750.79480.84","article-title":"Maternal morbidity associated with 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