{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T13:48:27Z","timestamp":1786974507537,"version":"build-2736575974"},"reference-count":24,"publisher":"Georg Thieme Verlag KG","issue":"06","license":[{"start":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T00:00:00Z","timestamp":1610582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Background and Purpose\u2003Accurate and rapid assessment of coagulation status is necessary to guide thrombolysis or reversal of anticoagulation in stroke patients, but commercially available point-of-care (POC) assays are not suited for coagulation testing in patients treated with direct oral anticoagulants (DOACs). We aimed to evaluate the direct thrombin monitoring (DTM) test card by Helena Laboratories (Texas, United States) for anti-IIa-specific POC coagulation testing, hypothesizing that its POC-ecarin clotting time (POC-ECT) accurately reflects dabigatran plasma concentrations.<\/jats:p><jats:p>\n          Methods\u2003A prospective single-center diagnostic study (ClinicalTrials.gov-identifier: NCT02825394) was conducted enrolling patients receiving a first dose of dabigatran and patients already on dabigatran treatment. Blood samples were collected before drug intake and 0.5, 1, 2, 8, and 12\u2009hours after intake. POC-ECT was performed using whole blood (WB), citrated blood (CB), and citrated plasma (CP). Dabigatran plasma concentrations were determined by mass spectrometry.<\/jats:p><jats:p>\n          Results\u2003In total, 240 blood samples from 40 patients contained 0 to 275\u2009ng\/mL of dabigatran. POC-ECT with WB\/CB\/CP ranged from 20 to 186\/184\/316\u2009seconds. Pearson's correlation coefficient showed a strong correlation between dabigatran concentrations and POC-ECT with WB\/CB\/CP (R2\n          \u2009=\u20090.78\/0.90\/0.92). Dabigatran concentrations &gt;30 and &gt;50\u2009ng\/mL (thresholds for thrombolysis, surgery, and reversal therapy according to clinical guidelines) were detected by POC-ECT with WB\/CB\/CP (&gt;36\/35\/45 and &gt;43\/45\/59\u2009seconds) with 95\/97\/97 and 96\/98\/97% sensitivity, and 81\/87\/94 and 74\/60\/91% specificity.<\/jats:p><jats:p>\n          Conclusion\u2003This first study evaluating DOAC-specific POC coagulation testing revealed an excellent correlation of POC-ECT with actual dabigatran concentrations. Detecting clinically relevant dabigatran levels with high sensitivity\/specificity, the DTM assay represents a suitable diagnostic tool in acute stroke, hemorrhage, and urgent surgery.<\/jats:p>","DOI":"10.1055\/s-0040-1721775","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:08:56Z","timestamp":1610669336000},"page":"782-791","source":"Crossref","is-referenced-by-count":9,"title":["Specific Point-of-Care Testing of Coagulation in Patients Treated with Dabigatran"],"prefix":"10.1055","volume":"121","author":[{"given":"Florian","family":"H\u00e4rtig","sequence":"additional","affiliation":[{"name":"Department of Neurology & Stroke, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Hertie Institute for Clinical Brain Research, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingvild","family":"Birschmann","sequence":"additional","affiliation":[{"name":"Institute for Laboratory and Transfusion Medicine, Heart and Diabetes Center, Ruhr University, Bad Oeynhausen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Peter","sequence":"additional","affiliation":[{"name":"German Centre for Diabetes Research (DZD), Neuherberg, Germany"},{"name":"Institute for Diabetes Research and Metabolic Diseases, Helmholtz Centre Munich, University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Institute for Clinical Chemistry and Pathobiochemistry, Department for Diagnostic Laboratory Medicine, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9111-1416","authenticated-orcid":false,"given":"Matthias","family":"Ebner","sequence":"additional","affiliation":[{"name":"Department of Neurology & Stroke, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Hertie Institute for Clinical Brain Research, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Department of Internal Medicine and Cardiology, Campus Virchow Hospital, Charit\u00e9, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Spencer","sequence":"additional","affiliation":[{"name":"Department of Neurology & Stroke, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Hertie Institute for Clinical Brain Research, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Gramlich","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hardy","family":"Richter","sequence":"additional","affiliation":[{"name":"Department of Neurology & Stroke, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Hertie Institute for Clinical Brain Research, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Institute for Laboratory and Transfusion Medicine, Heart and Diabetes Center, Ruhr University, Bad Oeynhausen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Lehmann","sequence":"additional","affiliation":[{"name":"German Centre for Diabetes Research (DZD), Neuherberg, Germany"},{"name":"Institute for Diabetes Research and Metabolic Diseases, Helmholtz Centre Munich, University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Institute for Clinical Chemistry and Pathobiochemistry, Department for Diagnostic Laboratory Medicine, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnar","family":"Blumenstock","sequence":"additional","affiliation":[{"name":"Department of Clinical Epidemiology and Applied Biometry, University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine S.","family":"Zuern","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital T\u00fcbingen, Germany"},{"name":"Department of Cardiology and Cardiovascular Research Institute Basel, University Hospital Basel, University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf","family":"Ziemann","sequence":"additional","affiliation":[{"name":"Department of Neurology & Stroke, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Hertie Institute for Clinical Brain Research, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Poli","sequence":"additional","affiliation":[{"name":"Department of Neurology & Stroke, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Hertie Institute for Clinical Brain Research, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2021,1,14]]},"reference":[{"issue":"03","key":"ref1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1002\/ana.22355","article-title":"Point-of-care laboratory halves door-to-therapy-decision time in acute stroke","volume":"69","author":"S Walter","year":"2011","journal-title":"Ann 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