{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:23:51Z","timestamp":1791487431903,"version":"4.3.4"},"reference-count":40,"publisher":"Scientific Scholar","issue":"02","license":[{"start":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T00:00:00Z","timestamp":1625788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background\u2003Alcoholic liver disease (ALD) is a major source of alcohol-related morbidity and mortality. Heavy drinkers and alcoholics may progress from fatty liver to alcoholic hepatitis to cirrhosis. The enzyme \u03b3-glutamyltranspeptidase (GGT) is a membrane-bound glycoprotein which catalyzes the transfer of the \u03b3-glutamyl group from \u03b3-glutamyl peptides to other peptides, amino acids, and water. Serum GGT activity mainly attributed to hepatobiliary system and thus is an important marker of ALD. Hence the present study is conducted to estimate and correlate the levels of GGT and osteopontin (OPN) in ALD.<\/jats:p><jats:p>Aims and Objectives\u2003The objective of this study is to estimate and correlate the levels of GGT and OPN in ALD.<\/jats:p><jats:p>Materials and Methods\u2003Sixty clinically diagnosed cases of ALD and sixty age- and gender-matched healthy controls were recruited for the study. Blood samples were collected from them and serum aspartate aminotransferase, serum alanine transaminases (ALTs), serum ALP levels, and plasma OPN levels were measured. Estimation of serum aspartate transaminases (AST), ALTs, and alkaline phosphatase (ALP) was assayed by standard photometric methods in autoanalyzer ERBA-XL (EM-200) using commercially available kits. OPN was estimated by using commercial kit based on enzyme-linked immunosorbent assay.<\/jats:p><jats:p>Results\u2003The parameters of the liver function tests such as AST, ALT, and ALP were significantly increased in patients with ALD (p\u2009&lt;\u20090.001) when compared with the healthy control subjects. In the present study, significantly increased levels of \u03b3-glutamyl transferases and OPN were found in patients with ALD (p\u2009&lt;\u20090.001) when compared with the control subjects. OPN showed significant positive correlations with AST (r\u2009=\u20090.76, p\u2009&lt;\u20090.001), ALT (r\u2009=\u20090.64, p\u2009&lt;\u20090.001), ALP (r\u2009=\u20090.68, p\u2009&lt;\u20090.001), and GGT (r\u2009=\u20090.61, p\u2009&lt;\u20090.001).<\/jats:p><jats:p>Conclusion\u2003The present study focuses on the role of GGT and OPN that are sensitive indicators of liver cell injury and are most helpful in recognizing hepatocellular diseases such as ALD, hepatitis, and liver cirrhosis. Hence, the pattern of the GGT and OPN levels elevation can be helpful diagnostically.<\/jats:p>","DOI":"10.1055\/s-0041-1729479","type":"journal-article","created":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T23:55:02Z","timestamp":1625874902000},"page":"101-108","source":"Crossref","is-referenced-by-count":8,"title":["A Study on the Serum \u03b3-Glutamyltranspeptidase and Plasma Osteopontin in Alcoholic Liver Disease"],"prefix":"10.25259","volume":"14","author":[{"given":"Arpita","family":"Suri","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, SGT University, Gurugram, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, SGT University, Gurugram, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjiv Kumar","family":"Bansal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, SGT University, Gurugram, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2021,7,9]]},"reference":[{"issue":"05","key":"ref1","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1111\/j.1530-0277.1993.tb05673.x","article-title":"Alcohol consumption and alcoholic liver disease: evidence of a threshold level of effects of ethanol","volume":"17","author":"V T Savolainen","year":"1993","journal-title":"Alcohol Clin Exp Res"},{"issue":"04","key":"ref2","doi-asserted-by":"crossref","first-page":"184","DOI":"10.2302\/kjm.48.184","article-title":"Pathogenesis of alcoholic liver disease: newer mechanisms of injury","volume":"48","author":"D W Crabb","year":"1999","journal-title":"Keio J Med"},{"issue":"03","key":"ref3","first-page":"209","article-title":"The epidemiology of alcoholic liver disease","volume":"27","author":"R E Mann","year":"2003","journal-title":"Alcohol Res Health"},{"issue":"03","key":"ref4","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1055\/s-2008-1040605","article-title":"Histologic spectrum of alcoholic liver disease","volume":"6","author":"R N MacSween","year":"1986","journal-title":"Semin Liver Dis"},{"issue":"01","key":"ref5","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.cld.2004.11.001","article-title":"Morphology of alcoholic liver disease","volume":"9","author":"J H Lefkowitch","year":"2005","journal-title":"Clin Liver Dis"},{"issue":"03","key":"ref6","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1055\/s-2004-832936","article-title":"Epidemiology of alcoholic liver disease","volume":"24","author":"S Mandayam","year":"2004","journal-title":"Semin Liver Dis"},{"issue":"06","key":"ref7","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1136\/gut.41.6.845","article-title":"The Dionysos Study Group. Drinking habits as cofactors of risk for alcohol induced liver damage","volume":"41","author":"S Bellentani","year":"1997","journal-title":"Gut"},{"issue":"02","key":"ref8","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/bjp.2008.30","article-title":"Neuropharmacology of alcohol addiction","volume":"154","author":"V Vengeliene","year":"2008","journal-title":"Br J Pharmacol"},{"issue":"07","key":"ref9","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s12098-007-0118-7","article-title":"Liver function tests and their interpretation","volume":"74","author":"B R Thapa","year":"2007","journal-title":"Indian J Pediatr"},{"issue":"02","key":"ref10","first-page":"110","article-title":"Gamma-glutamyl-transpeptidase in hepato-biliary disease\u2014value as an enzymatic liver function test","volume":"55","author":"A Cuschieri","year":"1974","journal-title":"Br J Exp Pathol"},{"key":"ref11","first-page":"604","author":"P Mauro","year":"2006"},{"issue":"01","key":"ref12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/hepr.12166","article-title":"Osteopontin: versatile modulator of liver diseases","volume":"44","author":"S Nagoshi","year":"2014","journal-title":"Hepatol Res"},{"issue":"09","key":"ref13","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.7150\/ijbs.16445","article-title":"Role of osteopontin in liver diseases","volume":"12","author":"Y Wen","year":"2016","journal-title":"Int J Biol Sci"},{"issue":"23","key":"ref14","doi-asserted-by":"crossref","first-page":"8819","DOI":"10.1073\/pnas.83.23.8819","article-title":"Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell-binding sequence","volume":"83","author":"A Oldberg","year":"1986","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"44","key":"ref15","doi-asserted-by":"crossref","first-page":"26232","DOI":"10.1074\/jbc.270.44.26232","article-title":"A biochemical characterization of the binding of osteopontin to integrins alpha v beta 1 and alpha v beta 5","volume":"270","author":"D D Hu","year":"1995","journal-title":"J Biol Chem"},{"issue":"06","key":"ref16","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1091\/mbc.9.6.1425","article-title":"Identification of osteopontin as a novel ligand for the integrin alpha8 beta1 and potential roles for this integrin-ligand interaction in kidney morphogenesis","volume":"9","author":"S Denda","year":"1998","journal-title":"Mol Biol Cell"},{"key":"ref17","first-page":"1767","author":"C Diana Nicoll","year":"2007"},{"issue":"04","key":"ref18","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/0092-8674(79)90103-X","article-title":"Transformed mammalian cells secrete specific proteins and phosphoproteins","volume":"16","author":"D R Senger","year":"1979","journal-title":"Cell"},{"issue":"04","key":"ref19","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.0954-6820.1970.tb08034.x","article-title":"Multiple serum enzyme analyses in chronic alcoholics","volume":"188","author":"A Konttinen","year":"1970","journal-title":"Acta Med Scand"},{"issue":"06","key":"ref20","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1016\/S0016-5085(19)30490-1","article-title":"Hepatic transaminase activity in alcoholic liver disease","volume":"78","author":"D S Matloff","year":"1980","journal-title":"Gastroe-nterology"},{"issue":"03","key":"ref21","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/BF01347885","article-title":"Serum gamma-glutamyl transpeptidase and chronic alcoholism","volume":"30","author":"S N Moussavian","year":"1985","journal-title":"Dig Dis Sci"},{"issue":"01","key":"ref22","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/BF02893039","article-title":"Biochemical diagnosis of alcoholism","volume":"20","author":"S K Das","year":"2005","journal-title":"Indian J Clin Biochem"},{"key":"ref23","first-page":"183","article-title":"Erythrocyte lipid peroxidation and antioxidants in chronic alcoholics with alcoholic liver disease","volume":"3","author":"R Maithreyi","year":"2010","journal-title":"Asian J Pharm Clin Res"},{"issue":"04","key":"ref24","first-page":"147","article-title":"Curative effect of garlic on alcoholic liver diseased patients","volume":"3","author":"S Mirunalini","year":"2010","journal-title":"Jordan J Biol Sci"},{"issue":"01","key":"ref25","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/1471-230X-12-145","article-title":"Effects of coffee, smoking, and alcohol on liver function tests: a comprehensive cross-sectional study","volume":"12","author":"E S Jang","year":"2012","journal-title":"BMC Gastroenterol"},{"issue":"05","key":"ref26","first-page":"638","article-title":"The effect of chronic liver diseases on some biochemical parameters in patients serum","volume":"4","author":"E F Al-Jumaily","year":"2012","journal-title":"Curr Res J Biol Sci"},{"issue":"02","key":"ref27","first-page":"280","article-title":"Comparative levels of ALT, AST, ALP and GGT in liver associated diseases","volume":"3","author":"M A Hyder","year":"2013","journal-title":"Eur J Exp Biol"},{"issue":"04","key":"ref28","first-page":"1099","article-title":"Comparative levels of liver enzymes in patients with various liver disorders","volume":"6","author":"B Gayathri","year":"2015","journal-title":"Int J Pharma Bio Sci"},{"issue":"02","key":"ref29","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.jhep.2018.03.031","article-title":"Dysregulation of serum bile acids and FGF19 in alcoholic hepatitis","volume":"69","author":"K Brandl","year":"2018","journal-title":"J Hepatol"},{"issue":"10","key":"ref30","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1046\/j.1440-1746.2001.02603.x","article-title":"Alcoholic liver disease: a clinical series in an Australian private practice","volume":"16","author":"K J Hourigan","year":"2001","journal-title":"J Gastroenterol Hepatol"},{"issue":"36","key":"ref31","doi-asserted-by":"crossref","first-page":"13088","DOI":"10.3748\/wjg.v20.i36.13088","article-title":"Osteopontin is an important mediator of alcoholic liver disease via hepatic stellate cell activation","volume":"20","author":"D Seth","year":"2014","journal-title":"World J Gastroenterol"},{"issue":"07","key":"ref32","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1136\/gut.24.7.625","article-title":"Hepatic gamma-glutamyltransferase activity in alcoholic fatty liver: comparison with other liver enzymes in man and rats","volume":"24","author":"R Teschke","year":"1983","journal-title":"Gut"},{"issue":"05","key":"ref33","doi-asserted-by":"crossref","first-page":"72","DOI":"10.9790\/0853-0757275","article-title":"Comparative study of ALT, AST, GGT & uric acid levels in liver diseases","volume":"7","author":"G V Benerji","year":"2013","journal-title":"IOSR-JDMS"},{"key":"ref34","first-page":"15","article-title":"Assessing alcohol intake & its dose dependent effects on liver enzymes by 24-h recall and questionnaire using NHANES 2001-2010 data","author":"S Agarwal","year":"2016","journal-title":"Nutrition Journal"},{"issue":"01","key":"ref35","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1080\/00365520510024061","article-title":"Serum osteopontin levels in patients with acute liver dysfunction","volume":"41","author":"M Arai","year":"2006","journal-title":"Scand J 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