{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T13:27:46Z","timestamp":1772803666632,"version":"3.50.1"},"reference-count":27,"publisher":"Scientific Scholar","issue":"02","license":[{"start":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T00:00:00Z","timestamp":1627516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objectives\u2003Amoebiasis is caused by the most common intestinal protozoan parasite Entamoeba histolytica. This parasite causes amoebic colitis, which is manifested by diarrhea, followed by dysentery. The laboratory diagnosis of intestinal amoebiasis in most cases is by microscopic examination of stool samples. Other nonroutine methods include coproantigen enzyme-linked immunosorbent assay (ELISA) from stool samples, serum ELISA for antibodies, stool culture, isoenzyme analysis, and polymerase chain reaction (PCR). The present study aimed to comparatively analyze the different diagnostic modalities used for the detection of E. histolytica from the stool sample of patients with intestinal amoebiasis.<\/jats:p><jats:p>\n          Materials and Methods\u2003This study was undertaken with 631 patients, during a period of 3 years, from January 2017 to December 2019. Stool specimen obtained from each patient was subjected to direct microscopic wet mount examination, coproantigen ELISA, and nested multiplex PCR, respectively.<\/jats:p><jats:p>\n          Results\u2003Out of all the patients tested, 5.2% were positive for E. histolytica. Among the positive cases, stool microscopy was positive in 3.17%, coproantigen ELISA was positive in 29 (4.6%) cases, and PCR was positive in 30 (4.75%) cases.<\/jats:p><jats:p>\n          Statistical Analysis\u2003The prevalence of E. histolytica infection was summarized as percentages. The three diagnostic tests done were statistically analyzed, taking microscopy as the gold standard. The agreement between techniques (microscopy, coproantigen ELISA, and PCR) was analyzed with kappa statistics. Sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy were summarized as percentage with 95% confidence interval.<\/jats:p><jats:p>\n          Conclusion\u2003In all suspected amoebiasis cases, a combination of stool microscopy, coproantigen testing with molecular detection of the parasite offers the best approach to diagnosis of this parasitic infection.<\/jats:p>","DOI":"10.1055\/s-0041-1732488","type":"journal-article","created":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T05:28:04Z","timestamp":1627622884000},"page":"125-131","source":"Crossref","is-referenced-by-count":7,"title":["A Comparative Analysis of Microscopy, Coproantigen Serology, and Nested Multiplex PCR in the Laboratory Diagnosis of Entamoeba histolytica Infection"],"prefix":"10.25259","volume":"14","author":[{"given":"Sindhusuta","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nonika","family":"Rajkumari","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anitha","family":"Gunalan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhanalakshmi","family":"Rajavelu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeby Jose","family":"Olickal","sequence":"additional","affiliation":[{"name":"Department of Preventive and Social Medicine, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2021,7,29]]},"reference":[{"key":"ref1","author":"H Zulfiqar"},{"issue":"03","key":"ref2","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1128\/CMR.00004-07","article-title":"Laboratory diagnostic techniques for Entamoeba species","volume":"20","author":"R Fotedar","year":"2007","journal-title":"Clin Microbiol Rev"},{"key":"ref3","author":"P P Singh","year":"2018"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"4601420","DOI":"10.1155\/2018\/4601420","article-title":"Entamoeba histolytica: updates in clinical manifestation, pathogenesis, and vaccine development","volume":"2018","author":"M Kantor","year":"2018","journal-title":"Can J Gastroenterol Hepatol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1016\/S0140-6736(16)31012-1","article-title":"GBD 2015 Mortality and Causes of Death Collaborators. 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