{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:20:17Z","timestamp":1772137217580,"version":"3.50.1"},"reference-count":25,"publisher":"Georg Thieme Verlag KG","issue":"10","funder":[{"DOI":"10.13039\/100010447","name":"German Center for Cardiovascular Research","doi-asserted-by":"crossref","award":["81X3600606"],"award-info":[{"award-number":["81X3600606"]}],"id":[{"id":"10.13039\/100010447","id-type":"DOI","asserted-by":"crossref"}]},{"name":"BMBF grant Sys_CARE","award":["01ZX1908A"],"award-info":[{"award-number":["01ZX1908A"]}]},{"name":"Bavarian State Ministry of Science and the Arts as part of the Bavarian Research Institute for Digital Transformation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection induces a coagulopathy characterized by platelet activation and a hypercoagulable state with an increased incidence of cardiovascular events. The viral spike protein S has been reported to enhance thrombosis formation, stimulate platelets to release procoagulant factors, and promote the formation of platelet\u2013leukocyte aggregates even in absence of the virus. Although SARS-CoV-2 vaccines induce spike protein overexpression to trigger SARS-CoV-2-specific immune protection, thrombocyte activity has not been investigated in this context. Here, we provide the first phenotypic platelet characterization of healthy human subjects undergoing BNT162b2 vaccination. Using mass cytometry, we analyzed the expression of constitutive transmembrane receptors, adhesion proteins, and platelet activation markers in 12 healthy donors before and at five different time points within 4 weeks after the first BNT162b2 administration. We measured platelet reactivity by stimulating thrombocyte activation with thrombin receptor-activating peptide. Activation marker expression (P-selectin, LAMP-3, LAMP-1, CD40L, and PAC-1) did not change after vaccination. All investigated constitutive transmembrane proteins showed similar expressions over time. Platelet reactivity was not altered after BNT162b2 administration. Activation marker expression was significantly lower compared with an independent cohort of mild symptomatic COVID-19 patients analyzed with the same platform. This study reveals that BNT162b2 administration does not alter platelet protein expression and reactivity.<\/jats:p>","DOI":"10.1055\/s-0041-1733934","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T18:45:03Z","timestamp":1628880303000},"page":"1706-1711","source":"Crossref","is-referenced-by-count":11,"title":["Platelet Surface Protein Expression and Reactivity upon TRAP Stimulation after BNT162b2 Vaccination"],"prefix":"10.1055","volume":"122","author":[{"given":"Melissa","family":"Klug","sequence":"first","affiliation":[{"name":"Department of Internal Medicine I, School of Medicine, University Hospital rechts der Isar, Technical University of Munich, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany"},{"name":"Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Lazareva","sequence":"first","affiliation":[{"name":"Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kilian","family":"Kirmes","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, School of Medicine, University Hospital rechts der Isar, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Rosenbaum","sequence":"additional","affiliation":[{"name":"School of Medicine, Institute of Clinical Chemistry and Pathobiochemistry, Technical University of Munich, Munich, Germany"},{"name":"TranslaTUM, Center for Translational Cancer Research, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Lukas","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, School of Medicine, University hospital rechts der Isar, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Weidlich","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, School of Medicine, University hospital rechts der Isar, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph D.","family":"Spinner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, School of Medicine, University hospital rechts der Isar, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moritz","family":"von Scheidt","sequence":"additional","affiliation":[{"name":"German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany"},{"name":"Deutsches Herzzentrum M\u00fcnchen, Cardiology, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosanna","family":"Gosetti","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, School of Medicine, University Hospital rechts der Isar, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Baumbach","sequence":"additional","affiliation":[{"name":"Chair of Computational Systems Biology, University of Hamburg, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Ruland","sequence":"additional","affiliation":[{"name":"School of Medicine, Institute of Clinical Chemistry and Pathobiochemistry, Technical University of Munich, Munich, Germany"},{"name":"TranslaTUM, Center for Translational Cancer Research, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianluigi","family":"Condorelli","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, Humanitas Clinical and Research Center IRCCS and Humanitas University, Rozzano, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl-Ludwig","family":"Laugwitz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, School of Medicine, University Hospital rechts der Isar, Technical University of Munich, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"List","sequence":"additional","affiliation":[{"name":"Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabell","family":"Bernlochner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, School of Medicine, University Hospital rechts der Isar, Technical University of Munich, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dario","family":"Bongiovanni","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, School of Medicine, University Hospital rechts der Isar, Technical University of Munich, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany"},{"name":"Department of Cardiovascular Medicine, Humanitas Clinical and Research Center IRCCS and Humanitas University, Rozzano, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2021,8,13]]},"reference":[{"issue":"04","key":"ref1","doi-asserted-by":"crossref","first-page":"268","DOI":"10.7326\/M20-2003","article-title":"Autopsy findings and venous thromboembolism in patients with COVID-19: a prospective cohort study","volume":"173","author":"D Wichmann","year":"2020","journal-title":"Ann Intern Med"},{"issue":"02","key":"ref2","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1161\/CIRCULATIONAHA.120.047430","article-title":"Pulmonary embolism in patients with COVID-19: awareness of an increased prevalence","volume":"142","author":"J Poissy","year":"2020","journal-title":"Circulation"},{"issue":"25","key":"ref3","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1056\/NEJMc2009020","article-title":"ST-segment elevation in patients with Covid-19 - a case series","volume":"382","author":"S 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Nicolai","year":"2020","journal-title":"Circulation"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1161\/CIRCRESAHA.120.317703","article-title":"Platelets can associate with SARS-Cov-2 RNA and are hyperactivated in COVID-19","volume":"127","author":"Y Zaid","year":"2020","journal-title":"Circ Res"},{"issue":"01","key":"ref8","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/s41419-020-03333-9","article-title":"SARS-CoV-2 infection is associated with a pro-thrombotic platelet phenotype","volume":"12","author":"D Bongiovanni","year":"2021","journal-title":"Cell Death Dis"},{"issue":"01","key":"ref9","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1186\/s13045-020-00954-7","article-title":"SARS-CoV-2 binds platelet ACE2 to enhance thrombosis in COVID-19","volume":"13","author":"S Zhang","year":"2020","journal-title":"J Hematol 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Platelet surface marker analysis by mass cytometry","volume":"31","author":"T A Blair","year":"2019","journal-title":"Platelets"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"650129","DOI":"10.3389\/fmed.2021.650129","article-title":"Thrombotic events in COVID-19 are associated with a lower use of prophylactic anticoagulation before hospitalization and followed by decreases in platelet reactivity","volume":"8","author":"C C Clark","year":"2021","journal-title":"Front Med (Lausanne)"},{"issue":"11","key":"ref25","first-page":"1395","article-title":"Vaccine-induced immune thrombotic thrombocytopenia (VITT): targeting pathomechanisms with Bruton tyrosine kinase inhibitors","volume":"121","author":"P von Hundelshausen","year":"2022","journal-title":"Thromb Haemost"}],"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0041-1733934.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T19:55:21Z","timestamp":1664222121000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0041-1733934"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,13]]},"references-count":25,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,9,26]]},"published-print":{"date-parts":[[2022,10]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0041-1733934","archive":["Portico","CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.05.18.21257324","asserted-by":"object"}]},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,13]]}}}