{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:10:54Z","timestamp":1768003854158,"version":"3.49.0"},"reference-count":19,"publisher":"Georg Thieme Verlag KG","issue":"03","license":[{"start":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T00:00:00Z","timestamp":1635120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kemenristek\/BRIN 2020"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003This study aimed to validate the use of Ca-apt-1, an RNA aptamer, that we generated previously as a probe for immunostaining of Candida albicans in rat tongue paraffin-fixed tissue sections<\/jats:p><jats:p>\n          Material and Methods\u2003The performance of Ca-apt-1 as a detector molecule was compared with that of anti-C. albicans polyclonal antibody (PcAb), which was used as a positive control. Immunostaining images were visualized by light microscopy and were analyzed by using ImageJ software.<\/jats:p><jats:p>\n          Results\u2003Microscopic results demonstrated that Ca-apt-1 specifically recognized and immunostained C. albicans cells of rat tongue candidiasis, with a specificity comparable to that of PcAb. ImageJ analysis showed that the area (pixels) detected by Ca-apt-1 was wider than that detected by the antibody. This indicates that the binding affinity of Ca-apt-1 toward C. albicans was better than that of PcAb on paraffin-embedded tissues.<\/jats:p><jats:p>\n          Conclusion\u2003This study demonstrates that Ca-apt-1 can be used as a probe for immunostaining of fixed tissue sections for oral candidiasis diagnosis.<\/jats:p>","DOI":"10.1055\/s-0041-1735794","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T23:03:03Z","timestamp":1635202983000},"page":"543-548","source":"Crossref","is-referenced-by-count":3,"title":["Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue"],"prefix":"10.1055","volume":"16","author":[{"given":"Boy M.","family":"Bachtiar","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chatchawan","family":"Srisawat","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and NANOTEC-Mahidol, University Center of Excellence in Nanotechnology for Cancer Diagnosis and Treatment, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok Noi, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Retno Pudji","family":"Rahayu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Retno D.","family":"Soedjodono","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Arin","family":"Prabandari","sequence":"additional","affiliation":[{"name":"Primate Research Center, IPB University, Bogor, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Endang W.","family":"Bachtiar","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2021,10,25]]},"reference":[{"issue":"03","key":"ref1","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1055\/s-0040-1712779","article-title":"Quantification and pathogenicity of Candida albicans in denture-wearing and nondenture-wearing elderly","volume":"14","author":"B M Bachtiar","year":"2020","journal-title":"Eur J Dent"},{"issue":"05","key":"ref2","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/eos.12725","article-title":"Effect of cell-free spent media 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