{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T11:51:44Z","timestamp":1784289104500,"version":"3.55.0"},"reference-count":35,"publisher":"Federa\u00e7\u00e3o das Associa\u00e7\u00f5es de Ginecologia e Obstetr\u00edcia","issue":"10","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":46,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Bras Ginecol Obstet"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003To investigate whether patients with a previous recombinant follicle stimulating hormone (rFSH)-stimulated cycle would have improved outcomes with rFSH\u2009+\u2009recombinant luteinizing hormone (rLH) stimulation in the following cycle.<\/jats:p><jats:p>\n          Methods\u2003For the present retrospective case-control study, 228 cycles performed in 114 patients undergoing intracytoplasmic sperm injection (ICSI) between 2015 and 2018 in an in vitro fertilization (IVF) center were evaluated. Controlled ovarian stimulation (COS) was achieved with rFSH (Gonal-f, Serono, Geneva, Switzerland) in the first ICSI cycle (rFSH group), and with rFSH and rLH (Pergoveris, Merck Serono S.p.A, Bari, Italy) in the second cycle (rFSH\u2009+\u2009rLH group). The ICSI outcomes were compared among the groups.<\/jats:p><jats:p>\n          Results\u2003Higher estradiol levels, oocyte yield, day-3 high-quality embryos rate and implantation rate, and a lower miscarriage rate were observed in the rFSH\u2009+\u2009rLH group compared with the rFSH group. In patients\u2009&lt;\u200935 years old, the implantation rate was higher in the rFSH\u2009+\u2009rLH group compared with the rFSH group. In patients \u2265 35 years old, higher estradiol levels, oocyte yield, day-3 high-quality embryos rate, and implantation rate were observed in the rFSH\u2009+\u2009rLH group. In patients with \u2264 4 retrieved oocytes, oocyte yield, mature oocytes rate, normal cleavage speed, implantation rate, and miscarriage rate were improved in the rFSH\u2009+\u2009rLH group. In patients with \u2265 5 retrieved oocytes, higher estradiol levels, oocyte yield, and implantation rate were observed in the rFSH\u2009+\u2009rLH group.<\/jats:p><jats:p>\n          Conclusion\u2003Ovarian stimulation with luteinizing hormone (LH) supplementation results in higher implantation rates, independent of maternal age and response to COS when compared with previous cycles stimulated with rFSH only. Improvements were also observed for ICSI outcomes and miscarriage after stratification by age and retrieved oocytes.<\/jats:p>","DOI":"10.1055\/s-0041-1736306","type":"journal-article","created":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T23:42:11Z","timestamp":1637106131000},"page":"749-758","source":"Crossref","is-referenced-by-count":6,"title":["Improving Implantation Rate in 2nd ICSI Cycle through Ovarian Stimulation with FSH and LH in GNRH Antagonist Regimen"],"prefix":"10.61622","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7869-5285","authenticated-orcid":false,"given":"Amanda Souza","family":"Setti","sequence":"additional","affiliation":[{"name":"Fertility Medical Group, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1333-6593","authenticated-orcid":false,"given":"Daniela Paes de Almeida Ferreira","family":"Braga","sequence":"additional","affiliation":[{"name":"Fertility Medical Group, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1379-2452","authenticated-orcid":false,"suffix":"J\u00fanior","given":"Assumpto","family":"Iaconelli","sequence":"additional","affiliation":[{"name":"Fertility Medical Group, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4971-1925","authenticated-orcid":false,"suffix":"J\u00fanior","given":"Edson","family":"Borges","sequence":"additional","affiliation":[{"name":"Fertility Medical Group, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"47794","published-online":{"date-parts":[[2021,11,16]]},"reference":[{"issue":"04","key":"ref1","doi-asserted-by":"publisher","first-page":"227","DOI":"10.4103\/0974-1208.126285","article-title":"Luteinizing hormone and follicle stimulating hormone synergy: A review of role in controlled ovarian hyper-stimulation","volume":"6","author":"G A Raju","year":"2013","journal-title":"J Hum Reprod Sci"},{"issue":"02","key":"ref2","doi-asserted-by":"publisher","first-page":"CD005070","DOI":"10.1002\/14651858.CD005070.pub2","article-title":"Recombinant Luteinizing Hormone (rLH) for controlled ovarian hyperstimulation in assisted reproductive cycles","author":"M H Mochtar","year":"2007","journal-title":"Cochrane Database Syst Rev"},{"issue":"05","key":"ref3","doi-asserted-by":"publisher","first-page":"1108","DOI":"10.1016\/j.fertnstert.2012.01.130","article-title":"The use of recombinant luteinizing hormone in patients undergoing assisted reproductive techniques with advanced reproductive age: a systematic review and meta-analysis","volume":"97","author":"M J Hill","year":"2012","journal-title":"Fertil Steril"},{"issue":"S 01","key":"ref4","doi-asserted-by":"publisher","first-page":"S33","DOI":"10.1016\/S1472-6483(11)60007-2","article-title":"Who needs LH in ovarian stimulation?","volume":"22","author":"C Alviggi","year":"2011","journal-title":"Reprod Biomed Online"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/1477-7827-10-9","article-title":"Hormonal, functional and genetic biomarkers in controlled ovarian stimulation: tools for matching patients and protocols","volume":"10","author":"C Alviggi","year":"2012","journal-title":"Reprod Biol Endocrinol"},{"issue":"03","key":"ref6","doi-asserted-by":"publisher","first-page":"1031","DOI":"10.1016\/j.fertnstert.2010.10.021","article-title":"Impact of luteinizing hormone administration on gonadotropin-releasing hormone antagonist cycles: an age-adjusted analysis","volume":"95","author":"E Bosch","year":"2011","journal-title":"Fertil Steril"},{"issue":"03","key":"ref7","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.rbmo.2011.12.005","article-title":"Does exogenous LH in ovarian stimulation improve assisted reproduction success? 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