{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:59:32Z","timestamp":1791313172559,"version":"4.3.1"},"reference-count":41,"publisher":"Federa\u00e7\u00e3o das Associa\u00e7\u00f5es de Ginecologia e Obstetr\u00edcia","issue":"02","license":[{"start":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T00:00:00Z","timestamp":1645747200000},"content-version":"vor","delay-in-days":24,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Bras Ginecol Obstet"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Objective\u2003Premature ovarian insufficiency (POI) contributes significantly to female infertility. Cyclophosphamide (CYC has adverse effects on folliculogenesis. Platelet-rich plasma (PRP) is an autologous product rich in many growth factors. We evaluated the protective effect of PRP on in vitro fertilization in female rats with CYC-induced ovarian damage.<\/jats:p>\n                  <jats:p>Methods\u2003Twenty-eight adult female Sprague-Dawley rats were randomly divided into four groups. Group 1 (control-sodium chloride 0.9%; 1\u2009mL\/kg, single-dose intraperitoneal [IP] injection); group 2 (CYC), 75\u2009mg\/kg, single-dose IP injection and sodium chloride 0.9% (1\u2009mL\/kg, single-dose IP injection); group 3 CYC plus PRP, CYC (75\u2009mg\/kg, single-dose and PRP (200 \u03bcl, single-dose) IP injection); and group 4 (PRP, 200 \u03bcl, single-dose IP injection).<\/jats:p>\n                  <jats:p>Results\u2003In the comparisons in terms of M1 and M2 oocytes, it was observed that the CYC group presented a significantly lower amount than the control, CYC\/PRP, and PRP groups. (for M1, p\u2009=\u20090.000, p\u2009=\u20090.029, p\u2009=\u20090.025; for M2, p\u2009=\u20090.009, p\u2009=\u20090.004, p\u2009=\u20090.000, respectively). The number of fertilized oocytes and two-celled good quality embryos was found to be statistically significant between the CYC and control groups, CYC\u2009+\u2009PRP and PRP groups (p\u2009=\u20090.009, p\u2009=\u20090.001, p\u2009=\u20090.000 for oocytes, respectively. For embryos; p\u2009=\u20090.016, p\u2009=\u20090.002, p\u2009=\u20090.000).<\/jats:p>\n                  <jats:p>Conclusion\u2003Platelet-rich plasma can protect the ovarian function against damage caused by CYC, and, in addition, it improves oocyte count and the development of embryos as a result of oocyte stimulation during the IVF procedure.<\/jats:p>","DOI":"10.1055\/s-0041-1741451","type":"journal-article","created":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T18:54:58Z","timestamp":1645815298000},"page":"161-168","source":"Crossref","is-referenced-by-count":10,"title":["Protective Effects of Platelet-rich plasma for in vitro Fertilization of Rats with Ovarian Failure Induced by Cyclophosphamide"],"prefix":"10.61622","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2617-3175","authenticated-orcid":false,"given":"\u00d6zcan","family":"Budak","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology and Artificial Reproductive Techniques, Faculty of Medicine, Sakarya University, Sakarya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4776-6244","authenticated-orcid":false,"given":"Mehmet S\u00fchha","family":"Bostanc\u0131","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology and Artificial Reproductive Techniques, Faculty of Medicine, Sakarya University, Sakarya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3280-851X","authenticated-orcid":false,"given":"Veysel","family":"Toprak","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Private Tatvan Can Hospital, Bitlis, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1730-1331","authenticated-orcid":false,"given":"Song\u00fcl","family":"Do\u011fanay","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Sakarya University, Sakarya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5656-6853","authenticated-orcid":false,"given":"Osman","family":"K\u00f6se","sequence":"additional","affiliation":[{"name":"Specialist of Gynecological Oncology, Department of Obstetrics and Gynecology Faculty of Medicine, Sakarya University, Sakarya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"47794","published-online":{"date-parts":[[2022,2,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1016\/S0140-6736(10)60355-8","article-title":"Primary ovarian insufficiency","volume":"376","author":"M De Vos","year":"2010","journal-title":"Lancet"},{"issue":"05","key":"ref2","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1530\/REP-09-0567","article-title":"Premature ovarian failure","volume":"140","author":"A N 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