{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:04:58Z","timestamp":1773965098248,"version":"3.50.1"},"reference-count":22,"publisher":"Federa\u00e7\u00e3o das Associa\u00e7\u00f5es de Ginecologia e Obstetr\u00edcia","issue":"03","license":[{"start":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T00:00:00Z","timestamp":1643587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Bras Ginecol Obstet"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003To assess the influence of oxidative stress on the gene expression of nitric oxide synthases (NOS 3 and NOS 2) and, hence, the cardiovascular responses in preeclampsia.<\/jats:p><jats:p>\n          Methods\u2003This was a case control study in which patients with preeclampsia (PE group) and normal pregnancy controls (NP group) were included according to the guidelines of the American College of Obstetricians and Gynecologists (ACOG). The serum levels of malondialdehyde (MDA), total antioxidant capacity, and nitric oxide (NO) were estimated, and the heart rate and mean arterial pressure were recorded. The gene profiling of NOS3 and NOS2 was performed through real-time polymerase chain reaction (RT-PCR). The statistical analysis was performed using the Student t-test, and values of p\u2009&lt;\u20090.05 were considered statistically significant.<\/jats:p><jats:p>\n          Results\u2003The serum levels of malondialdehyde were increased (p\u2009&lt;\u20090.0001), and the total antioxidant capacity was reduced in the PE group (p\u2009=\u20090.034), indicating oxidative stress. In the PE group, the mean arterial pressure was significantly higher (p\u2009&lt;\u20090.0001), but the serum levels of NO did not show a statistically significant reduction (p\u2009=\u20090.20). The gene expression profiling of NOS3 and NOS2 revealed a down regulation in the PE group by 8.49 and 51.05 times respectively.<\/jats:p><jats:p>\n          Conclusion\u2003Oxidative stress may lead to endothelial dysfunction, which could result in increased mean arterial pressure. Nitric oxide may play a role in this mechanism, but interactions with other vasoactive \/biological substances cannot be overlooked, as the gene expression of NOS3 and NOS2 has been reduced.<\/jats:p>","DOI":"10.1055\/s-0042-1742313","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:49:31Z","timestamp":1643676571000},"page":"214-219","source":"Crossref","is-referenced-by-count":3,"title":["Interplay of Oxidative Stress and Nitric Oxide Synthase Gene Expression on Cardiovascular Responses in Preeclampsia"],"prefix":"10.61622","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2140-4632","authenticated-orcid":false,"given":"Anita","family":"Herur","sequence":"additional","affiliation":[{"name":"Department of Physiology, Shri B. M. Patil Medical College, Hospital and Research Centre, BLDE (Deemed to be University), Vijayapur-586103, Karnataka, India"},{"name":"Department of Physiology and OBG, S. Nijalingappa Medical college, Bagalkot-587103, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8353-1541","authenticated-orcid":false,"given":"Manjunatha","family":"Aithala","sequence":"additional","affiliation":[{"name":"Department of Physiology, Shri B. M. Patil Medical College, Hospital and Research Centre, BLDE (Deemed to be University), Vijayapur-586103, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3641-2117","authenticated-orcid":false,"given":"Kusal K.","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Physiology, Shri B. M. Patil Medical College, Hospital and Research Centre, BLDE (Deemed to be University), Vijayapur-586103, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3779-189X","authenticated-orcid":false,"given":"Ashalata","family":"Mallapur","sequence":"additional","affiliation":[{"name":"Department of Physiology and OBG, S. Nijalingappa Medical college, Bagalkot-587103, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8211-5973","authenticated-orcid":false,"given":"Rajat","family":"Hegde","sequence":"additional","affiliation":[{"name":"Karnataka Institute for DNA Research (KIDNAR), Dharwad-580003, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9022-0052","authenticated-orcid":false,"given":"Suyamindra","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"Department of Physiology, Shri B. M. 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