{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T16:14:22Z","timestamp":1780071262299,"version":"3.54.0"},"reference-count":17,"publisher":"Federa\u00e7\u00e3o das Associa\u00e7\u00f5es de Ginecologia e Obstetr\u00edcia","issue":"03","license":[{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Brazil","award":["2018\u20130462"],"award-info":[{"award-number":["2018\u20130462"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Bras Ginecol Obstet"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003To evaluate the reproductive and histological characteristics of fresh cultured ovarian tissue from transgender male patients.<\/jats:p><jats:p>\n          Methods\u2003An in vitro pilot study in which samples were collected during sex reassignment surgery for transgender male patients. The ovarian cortex was cut into fragments of 2\u2009mm, 3\u2009mm, and 4\u2009mm, and placed in a 96-well plate suitable for cultivation at days 0, 2, 4, 6, and 8, when the histology was analyzed.<\/jats:p><jats:p>\n          Results\u2003Stromal hyperplasia was observed in all samples, and it was not associated with the obtainment of primordial or primary follicles. Peripheral reduction in cell count was also a recurrent finding. Primordial and primary follicles were identified with a heterogeneous pattern in fragments from the same patient and from different patients, and follicles in more advanced stages of development (secondary and antral) were not found. There was an association between the diameter of the ovarian fragments and the identification of primary follicles (p\u2009=\u20090.036). The number of days in culture was associated with histological signs of tissue damaging in the fragments (p\u2009=\u20090.002). The total number of follicles identified in the samples with 2\u2009mm in diameter was significantly lower than in those that measured 4\u2009mm in diameter (p\u2009=\u20090.031).<\/jats:p><jats:p>\n          Conclusion\u2003A diameter of 4\u2009mm is suitable for ovarian tissue culture with the benefit of ease of handling. Even after prolonged exposure to testosterone, the ovarian fragments presented primordial and primary follicles, maintaining viability throughout the days they were exposed to the culture. Freezing the ovarian cortex of transgender patients who will undergo surgery for gender reassignment would be an interesting option, in the future, for the preservation of fertility.<\/jats:p>","DOI":"10.1055\/s-0042-1742410","type":"journal-article","created":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T23:53:28Z","timestamp":1644450808000},"page":"251-257","source":"Crossref","is-referenced-by-count":4,"title":["Ovarian Tissue Culture to Preserve Fertility in Transgender Male Patients after Hormonal Treatment"],"prefix":"10.61622","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8961-2967","authenticated-orcid":false,"given":"Alessandra Leal","family":"Bottini","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ginecologia e Obstetr\u00edcia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8921-9214","authenticated-orcid":false,"given":"V\u00e2nia Marisia Santos Fortes dos","family":"Reis","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ginecologia e Obstetr\u00edcia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1039-7940","authenticated-orcid":false,"given":"Edison","family":"Capp","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ginecologia e Obstetr\u00edcia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"},{"name":"Department of Gynecology and Obstetrics, School of Medicine, Hospital de Cl\u00ednicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre\/RS, 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