{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T14:46:33Z","timestamp":1775486793781,"version":"3.50.1"},"reference-count":46,"publisher":"Scientific Scholar","issue":"03","license":[{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Department of Science & Technology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Introduction\u2003Pseudomonas aeruginosa and Acinetobacter baumannii are important pathogens in health care\u2013associated infections. Fluoroquinolone resistance has emerged in these pathogens. In this study, we aimed to determine the occurrence of plasmid-mediated quinolone resistance (PMQR) determinants (qnrA, qnrB, qnrS, aac(6\u2032)-Ib-cr, oqxAB, and qepA) by polymerase chain reaction (PCR) and the transmissibility of plasmid-borne resistance determinants in clinical isolates of P. aeruginosa and A. baumannii.<\/jats:p><jats:p>Materials and Methods\u2003The study included P. aeruginosa (85) and A. baumannii (45) which were nonduplicate, clinically significant, and ciprofloxacin resistant. Antibiotic susceptibility testing was done by disk diffusion method for other antimicrobial agents, namely amikacin, ceftazidime, piperacillin\/tazobactam, ofloxacin, levofloxacin, and imipenem. Minimum inhibitory concentration of ciprofloxacin was determined. Efflux pump activity was evaluated using carbonyl-cyanide m-chlorophenylhydrazone (CCCP). The presence of PMQR genes was screened by PCR amplification. Transferability of PMQR genes was determined by conjugation experiment, and plasmid-based replicon typing was performed.<\/jats:p><jats:p>Results\u2003Resistance to other classes of antimicrobial agents was as follows: ceftazidime (86.9%), piperacillin\/tazobactam (73.8%), imipenem (69.2%), and amikacin (63.8%). The minimal inhibitory concentration (MIC)50 and MIC90 for ciprofloxacin were 64 and greater than or equal to 256 \u00b5g\/mL, respectively. There was a reduction in MIC for 37 (28.4%) isolates with CCCP. In P. aeruginosa, 12 (14.1%) isolates harbored qnrB, 12 (14.1%) qnrS, 9 (10.5%) both qnrB and qnrS, 66 (77.6%) aac(6\u2032)-Ib-cr, and 3 (3.5%) oqxAB gene. In A. baumannii, qnrB was detected in 2 (4.4%), 1 (2.2%) harbored both the qnrA and qnrS, 1 isolate harbored qnrB and qnrS, 21 (46.6%) aac(6\u2032)-Ib-cr, and 1 (2.2%) isolate harbored oqxAB gene. Notably, qepA gene was not detected in any of the study isolates. Conjugation experiments revealed that 12 (9.2%) were transferable. Of the transconjugants, seven (58.3%) belonged to IncFII type plasmid replicon, followed by four (33.3%) IncA\/C and one (8.3%) IncFIC type.<\/jats:p><jats:p>Conclusion\u2003The plasmid-mediated resistance aac(6\u2032)-Ib-cr gene is primarily responsible for mediating fluoroquinolone resistance in clinical isolates of P. aeruginosa and A. baumannii. The predominant plasmid type is IncFII.<\/jats:p>","DOI":"10.1055\/s-0042-1742636","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:03:25Z","timestamp":1644451405000},"page":"271-277","source":"Crossref","is-referenced-by-count":25,"title":["Plasmid-Mediated Fluoroquinolone Resistance in Pseudomonas aeruginosa and Acinetobacter baumannii"],"prefix":"10.25259","volume":"14","author":[{"given":"Geetha P.","family":"Venkataramana","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aishwarya K.V.","family":"Lalitha","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanthi","family":"Mariappan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uma","family":"Sekar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2022,2,9]]},"reference":[{"issue":"07","key":"ref1","doi-asserted-by":"crossref","first-page":"2242","DOI":"10.1128\/AAC.47.7.2242-2248.2003","article-title":"Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China","volume":"47","author":"M Wang","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"S49","DOI":"10.1086\/504477","article-title":"Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa","volume":"43","author":"R A Bonomo","year":"2006","journal-title":"Clin Infect Dis"},{"issue":"01","key":"ref3","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/nyas.12830","article-title":"Mechanisms of drug resistance: quinolone resistance","volume":"1354","author":"D C Hooper","year":"2015","journal-title":"Ann N Y Acad Sci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/S0140-6736(97)07322-4","article-title":"Quinolone resistance from a transferable plasmid","volume":"351","author":"L Mart\u00ednez-Mart\u00ednez","year":"1998","journal-title":"Lancet"},{"issue":"10","key":"ref5","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S1473-3099(06)70599-0","article-title":"The worldwide emergence of plasmid-mediated quinolone resistance","volume":"6","author":"A Robicsek","year":"2006","journal-title":"Lancet Infect Dis"},{"issue":"09","key":"ref6","doi-asserted-by":"crossref","first-page":"3354","DOI":"10.1128\/AAC.00339-07","article-title":"New plasmid-mediated fluoroquinolone efflux pump, QepA, found in an Escherichia coli clinical isolate","volume":"51","author":"K Yamane","year":"2007","journal-title":"Antimicrob Agents Chemother"},{"issue":"05","key":"ref7","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/microbiolspec.PLAS-0006-2013","article-title":"Plasmid-mediated quinolone resistance","volume":"2","author":"G A Jacoby","year":"2014","journal-title":"Microbiol Spectr"},{"issue":"01","key":"ref8","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1093\/jac\/dks377","article-title":"Contribution of OqxAB efflux pumps to quinolone resistance in extended-spectrum-\u03b2-lactamase-producing Klebsiella pneumoniae","volume":"68","author":"J M Rodr\u00edguez-Mart\u00ednez","year":"2013","journal-title":"J Antimicrob Chemother"},{"issue":"02","key":"ref9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s10156-010-0120-2","article-title":"Plasmid-mediated quinolone resistance: an update","volume":"17","author":"J M Rodr\u00edguez-Mart\u00ednez","year":"2011","journal-title":"J Infect Chemother"},{"issue":"12","key":"ref10","doi-asserted-by":"crossref","first-page":"4268","DOI":"10.1128\/AAC.00830-08","article-title":"High prevalence of plasmid-mediated quinolone resistance genes qnr and aac(6\u2032)-Ib-cr in clinical isolates of Enterobacteriaceae from nine teaching hospitals in China","volume":"52","author":"H Yang","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"issue":"03","key":"ref11","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.diagmicrobio.2012.12.001","article-title":"Detection of plasmid-mediated quinolone resistance determinants and the emergence of multidrug resistance in clinical isolates of Shigella in SiXian area, China","volume":"75","author":"Y L Zhu","year":"2013","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"05","key":"ref12","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1179\/1973947815Y.0000000017","article-title":"Detection of the plasmid-mediated quinolone resistance determinants in clinical isolates of Acinetobacter baumannii in China","volume":"28","author":"H Yang","year":"2016","journal-title":"J Chemother"},{"issue":"03","key":"ref13","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.mimet.2005.03.018","article-title":"Identification of plasmids by PCR-based replicon typing","volume":"63","author":"A Carattoli","year":"2005","journal-title":"J Microbiol Methods"},{"key":"ref14","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing. M100","author":"Clinical and Laboratory Standards Institute","year":"2017","edition":"27th ed."},{"issue":"01","key":"ref15","doi-asserted-by":"crossref","first-page":"e8691","DOI":"10.5812\/jjm.8691","article-title":"Effect of efflux pump inhibitor carbonyl cyanide 3-chlorophenylhydrazone on the minimum inhibitory concentration of ciprofloxacin in Acinetobacter baumannii clinical isolates","volume":"7","author":"A Ardebili","year":"2014","journal-title":"Jundishapur J Microbiol"},{"issue":"08","key":"ref16","doi-asserted-by":"crossref","first-page":"2872","DOI":"10.1128\/AAC.01647-05","article-title":"qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States","volume":"50","author":"A Robicsek","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"issue":"05","key":"ref17","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.ijantimicag.2010.07.012","article-title":"Allele-specific polymerase chain reaction (PCR) for rapid detection of the aac(6\u2032)-Ib-cr quinolone resistance gene","volume":"36","author":"D W Wareham","year":"2010","journal-title":"Int J Antimicrob Agents"},{"issue":"01","key":"ref18","doi-asserted-by":"crossref","first-page":"42","DOI":"10.20546\/ijcmas.2017.601.006","article-title":"Plasmid mediated quinolone resistance determinants among nosocomial clinical Pseudomonas aeruginosa isolates","volume":"6","author":"M A Saleh","year":"2017","journal-title":"Int J Curr Microbiol Appl Sci"},{"issue":"07","key":"ref19","doi-asserted-by":"crossref","first-page":"2242","DOI":"10.1128\/AAC.47.7.2242-2248.2003","article-title":"Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China","volume":"47","author":"M Wang","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"ref20","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1039\/C9MD00120D","article-title":"Quinolone antibiotics","volume":"10","author":"T DM Pham","year":"2019","journal-title":"MedChemComm"},{"issue":"04","key":"ref21","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1097\/01.qco.0000171920.44809.f0","article-title":"Update on Pseudomonas aeruginosa and Acinetobacter baumannii infections in the healthcare setting","volume":"18","author":"S Navon-Venezia","year":"2005","journal-title":"Curr Opin Infect Dis"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"116","DOI":"10.2174\/1874285801812010116","article-title":"Molecular study of quinolone resistance determining regions of gyrA gene and parC genes in clinical isolates of Acintobacter baumannii resistant to fluoroquinolone","volume":"12","author":"M ES Zaki","year":"2018","journal-title":"Open Microbiol J"},{"issue":"06","key":"ref23","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1093\/jac\/dkl404","article-title":"Analysis of the mechanisms of fluoroquinolone resistance in urinary tract pathogens","volume":"58","author":"H Y Chenia","year":"2006","journal-title":"J Antimicrob Chemother"},{"issue":"06","key":"ref24","doi-asserted-by":"crossref","first-page":"e0178888","DOI":"10.1371\/journal.pone.0178888","article-title":"Genomic and phenotypic characterisation of fluoroquinolone resistance mechanisms in Enterobacteriaceae in Durban, South Africa","volume":"12","author":"J Osei Sekyere","year":"2017","journal-title":"PLoS One"},{"issue":"02","key":"ref25","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/j.1574-6976.2011.00290.x","article-title":"Broad-specificity efflux pumps and their role in multidrug resistance of Gram-negative bacteria","volume":"36","author":"H Nikaido","year":"2012","journal-title":"FEMS Microbiol Rev"},{"issue":"06","key":"ref26","doi-asserted-by":"crossref","first-page":"600","DOI":"10.3855\/jidc.7619","article-title":"Role of efflux pump inhibitor in decreasing antibiotic cross-resistance of Pseudomonas aeruginosa in a burn hospital in Iran","volume":"10","author":"M Talebi-Taher","year":"2016","journal-title":"J Infect Dev Ctries"},{"issue":"01","key":"ref27","doi-asserted-by":"crossref","first-page":"37","DOI":"10.4103\/ijabmr.IJABMR_90_19","article-title":"Detection of overexpression of efflux pump expression in fluoroquinolone-resistant Pseudomonas aeruginosa isolates","volume":"10","author":"N Al Rashed","year":"2020","journal-title":"Int J Appl Basic Med Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"479","DOI":"10.2147\/IDR.S147192","article-title":"Different phenotypic and molecular mechanisms associated with multidrug resistance in Gram-negative clinical isolates from Egypt","volume":"10","author":"O M Helmy","year":"2017","journal-title":"Infect Drug Resist"},{"issue":"04","key":"ref29","doi-asserted-by":"crossref","first-page":"99","DOI":"10.12816\/0025926","article-title":"Phenotypic and genotypic detection of some antimicrobial resistance mechanisms among multidrug-resistant Acinetobacter baumannii isolated from immunocompromised patients in Egypt","volume":"23","author":"F M Gomaa","year":"2014","journal-title":"Egypt J Med Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"915","DOI":"10.2147\/IDR.S203288","article-title":"Investigation of six plasmid-mediated quinolone resistance genes among clinical isolates of pseudomonas: a genotypic study in Saudi Arabia","volume":"12","author":"M F El-Badawy","year":"2019","journal-title":"Infect Drug Resist"},{"issue":"02","key":"ref31","first-page":"250","article-title":"Determination of Qnr allele frequencies in fluoroquinolone resistant Pseudomonas aeruginosa isolated from burn wounds","volume":"69","author":"K Rafiq","year":"2019","journal-title":"J Pak Med Assoc"},{"issue":"01","key":"ref32","first-page":"1386","article-title":"Prevalence and fluoroquinolone resistance of Pseudomonas aeruginosa in a hospital of South China","volume":"8","author":"X Yang","year":"2015","journal-title":"Int J Clin Exp Med"},{"issue":"04","key":"ref33","doi-asserted-by":"crossref","first-page":"310","DOI":"10.7883\/yoken.JJID.2008.310","article-title":"Investigation of plasmid-mediated quinolone resistance among isolates obtained in a Turkish intensive care unit","volume":"61","author":"H Nazik","year":"2008","journal-title":"Jpn J Infect Dis"},{"issue":"04","key":"ref34","first-page":"602","article-title":"Investigation of plasmid-mediated quinolone resistance in Pseudomonas aeruginosa strains isolated from cystic fibrosis patients [in Turkish]","volume":"45","author":"A Y Coban","year":"2011","journal-title":"Mikrobiyol Bul"},{"issue":"01","key":"ref35","doi-asserted-by":"crossref","first-page":"12268","DOI":"10.1038\/s41598-018-30756-4","article-title":"Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients","volume":"8","author":"S M Hamed","year":"2018","journal-title":"Sci Rep"},{"issue":"03","key":"ref36","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.diagmicrobio.2007.10.002","article-title":"First report of qnrB-producing Enterobacter cloacae and qnrA-producing Acinetobacter baumannii recovered from Algerian hospitals","volume":"60","author":"A Touati","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"05","key":"ref37","doi-asserted-by":"crossref","first-page":"e0155914","DOI":"10.1371\/journal.pone.0155914","article-title":"Clinical and molecular epidemiology of multidrug-resistant P. aeruginosa carrying aac(6\u2032)-Ib-cr, qnrS1 and blaSPM genes in Brazil","volume":"11","author":"B F Araujo","year":"2016","journal-title":"PLoS One"},{"issue":"03","key":"ref38","doi-asserted-by":"crossref","first-page":"285","DOI":"10.4103\/0255-0857.136567","article-title":"Investigation of plasmid-mediated quinolone resistance in Pseudomonas aeruginosa clinical isolates","volume":"32","author":"Y T Cayci","year":"2014","journal-title":"Indian J Med Microbiol"},{"issue":"03","key":"ref39","doi-asserted-by":"crossref","first-page":"109","DOI":"10.5812\/pedinfect.11392","article-title":"The first report of CMY, aac (6\u2032)-Ib and 16S rRNA methylase genes among Pseudomonas aeruginosa isolates from Iran","volume":"1","author":"M Jafari","year":"2013","journal-title":"Arch Pediatr Infect Dis"},{"issue":"02","key":"ref40","first-page":"130","article-title":"Detection of plasmid-mediated quinolone resistance determinants in clinical non-fermentative bacteria and ciprofloxacin sensitive Enterobacteriaceae strains","volume":"29","author":"Z Xue-qing","year":"2014","journal-title":"Dis Surveill"},{"issue":"04","key":"ref41","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1590\/S1517-83822014000400041","article-title":"Prevalence of resistance to aminoglycosides and fluoroquinolones among Pseudomonas aeruginosa strains in a University Hospital in Northeastern Poland","volume":"45","author":"A D Michalska","year":"2015","journal-title":"Braz J Microbiol"},{"issue":"03","key":"ref42","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.diagmicrobio.2014.03.025","article-title":"Emergence of plasmid-mediated quinolone resistance genes in clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa in Henan, China","volume":"79","author":"X Jiang","year":"2014","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"05","key":"ref43","doi-asserted-by":"crossref","first-page":"382","DOI":"10.3855\/jidc.2613","article-title":"Dissemination of IncF plasmids carrying beta-lactamase genes in Gram-negative bacteria from Nigerian hospitals","volume":"7","author":"D O Ogbolu","year":"2013","journal-title":"J Infect Dev Ctries"},{"issue":"04","key":"ref44","first-page":"298","article-title":"Full characterization of an IncR plasmid harboring qnrS1 recovered from a VIM-11-producing Pseudomonas aeruginosa","volume":"52","author":"A Elena","year":"2020","journal-title":"Rev Argent Microbiol"},{"issue":"03","key":"ref45","doi-asserted-by":"crossref","first-page":"322","DOI":"10.4103\/0377-4929.188108","article-title":"Distribution of plasmid-mediated quinolone resistance in Gram-negative bacteria from a tertiary hospital in Nigeria","volume":"59","author":"D O Ogbolu","year":"2016","journal-title":"Indian J Pathol Microbiol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"S367","DOI":"10.1093\/cid\/ciu706","article-title":"Update on Acinetobacter species: mechanisms of antimicrobial resistance and contemporary in vitro activity of minocycline and other treatment options","volume":"59","author":"M Castanheira","year":"2014","journal-title":"Clin Infect Dis"}],"container-title":["Journal of Laboratory 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