{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T21:12:41Z","timestamp":1793740361408,"version":"4.6.1"},"reference-count":37,"publisher":"Georg Thieme Verlag KG","issue":"05","funder":[{"name":"Department of Clinical Biochemistry, Aarhus University Hospital"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Semin Thromb Hemost"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Increased platelet activity is an important predictor for recurrent cardiovascular events in patients with acute coronary syndromes (ACS). Flow cytometry is an advanced method for evaluation of platelet activity. We aimed to summarize the current literature on dynamic changes in platelet activity analyzed by flow cytometry in patients with ACS. Employing the guidelines of Preferred Report Items for Systematic Reviews and Meta-Analyses (PRISMA), we searched PubMed and Embase on October 26, 2021, and identified studies measuring platelet activity with flow cytometry in ACS patients in the acute phase (baseline) and at follow-up in a more stable phase. In the 12 included studies, fibrinogen receptor, \u03b1-granule secretion, platelet reactivity index, monocyte-platelet aggregates, neutrophil-platelet aggregates, and reticulated platelets were measured. The fibrinogen receptor and \u03b1-granule secretion were either unchanged or lower during follow-up measurements than in the acute phase. Platelet reactivity index showed inconsistent results. Values of monocyte-platelet aggregates and neutrophil-platelet aggregates were lower at follow-up than at baseline (p-values &lt;0.05). Reticulated platelets were either unchanged (p-value &gt;0.64) or lower at 1 to 2 months follow-up (p-value 0.04), and also lower at 5 months to 1-year follow-up (p-value &gt;0.005) compared with baseline. Overall, flow cytometric analyses of platelet function in ACS patients showed that platelet activity was lower at follow-up than at baseline. However, in some patients, platelet activity remained unchanged from baseline to follow-up, possibly indicating a sustained high platelet activity that may increase the risk of recurrent cardiovascular events.<\/jats:p>","DOI":"10.1055\/s-0042-1742742","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:33:16Z","timestamp":1646094796000},"page":"542-551","source":"Crossref","is-referenced-by-count":5,"title":["Flow Cytometric Assessment of Changes in Platelet Reactivity after Acute Coronary Syndrome: A Systematic Review"],"prefix":"10.1055","volume":"48","author":[{"given":"Oliver Buchhave","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Thrombosis and Hemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Pasalic","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pathology and Medical Research, Departments of Clinical and Laboratory Hematology, Westmead University Hospital, Sydney, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter H.","family":"Nissen","sequence":"additional","affiliation":[{"name":"Thrombosis and Hemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik Lerkevang","family":"Grove","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steen Dalby","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Mette","family":"Hvas","sequence":"additional","affiliation":[{"name":"Thrombosis and Hemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"issue":"02","key":"ref1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/eurheartj\/ehx393","article-title":"2017 ESC Guidelines for the management of acute myocardial infarction in patients presenting with ST-segment elevation: The Task Force for the management of acute myocardial infarction in patients presenting with ST-segment elevation of the European Society of Cardiology (ESC)","volume":"39","author":"B Ibanez","year":"2018","journal-title":"Eur Heart J"},{"issue":"19","key":"ref2","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1093\/eurheartj\/ehu505","article-title":"Cardiovascular risk in post-myocardial infarction patients: nationwide real world data demonstrate the importance of a long-term perspective","volume":"36","author":"T Jernberg","year":"2015","journal-title":"Eur Heart J"},{"issue":"01","key":"ref3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1080\/09537104.2020.1763731","article-title":"Novel antiplatelet targets in the treatment of acute coronary syndromes","volume":"32","author":"F O 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Haemost"},{"issue":"03","key":"ref10","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1055\/s-0035-1570082","article-title":"Platelet function determined by flow cytometry: new perspectives?","volume":"42","author":"S Ramstr\u00f6m","year":"2016","journal-title":"Semin Thromb Hemost"},{"issue":"02","key":"ref11","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1080\/09537104.2017.1353684","article-title":"Platelet function investigation by flow cytometry: sample volume, needle size, and reference intervals","volume":"29","author":"O H Pedersen","year":"2018","journal-title":"Platelets"},{"issue":"01","key":"ref12","doi-asserted-by":"crossref","first-page":"66","DOI":"10.3109\/09537104.2015.1032919","article-title":"Investigation of platelet function and platelet disorders using flow cytometry","volume":"27","author":"P Rubak","year":"2016","journal-title":"Platelets"},{"issue":"06","key":"ref13","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1002\/cyto.b.21642","article-title":"Platelet characteristics in patients with essential thrombocytosis","volume":"94","author":"O H Pedersen","year":"2018","journal-title":"Cytometry B Clin Cytom"},{"issue":"07","key":"ref14","doi-asserted-by":"crossref","first-page":"e1000100","DOI":"10.1371\/journal.pmed.1000100","article-title":"The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: explanation and elaboration","volume":"6","author":"A Liberati","year":"2009","journal-title":"PLoS Med"},{"issue":"05","key":"ref16","doi-asserted-by":"crossref","first-page":"547","DOI":"10.5603\/CJ.a2014.0026","article-title":"Prasugrel overcomes high on-clopidogrel platelet reactivity in the acute phase of acute coronary syndrome and maintains its antiplatelet potency at 30-day 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