{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:59:15Z","timestamp":1782860355955,"version":"3.54.5"},"reference-count":13,"publisher":"Georg Thieme Verlag KG","issue":"05","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Pediatr Surg"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Introduction\u2003Treatment for long-gap esophageal atresia (LGEA) aims at achieving primary anastomosis with minimal tension. Previous studies have shown that intramural injections with botulinum toxin type-A (BTX-A) from the adventitial side can increase the elongation of the piglet and rat esophagus before bursting, and that this effect is dose and time dependent. Our aim was to determine if endoscopic injections would be feasible, safe, and with an effect on the mechanical properties of the esophagus.<\/jats:p><jats:p>\n          Methods\u2003Twenty-two male piglets (5.15\u2009kg) were randomized into two groups, one receiving 2 units\/kg BTX-A, the other equal volume 0.9% NaCl. On day 3, the esophagus was harvested and tested in a stretch-tension machine to evaluate elongation and maximum load, followed by histological examination.<\/jats:p><jats:p>\n          Results\u2003No adverse effects to the procedure were observed. No statistically significant difference in elongation or maximum load before bursting between the treatment and placebo group was found. In histopathological analysis, inflammation and abscess formation were observed with no statistically significant difference between the two groups.<\/jats:p><jats:p>\n          Conclusion\u2003Endoscopic placement of BTX-A injections in the piglet esophagus was safe and feasible but did not result in any difference in the mechanical properties or histology of the esophagus.<\/jats:p>","DOI":"10.1055\/s-0042-1745783","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T23:57:59Z","timestamp":1654905479000},"page":"460-464","source":"Crossref","is-referenced-by-count":3,"title":["Endoscopic Injections of Botulinum Toxin Type A in the Piglet Esophagus Is Safe and Feasible but Did Not Result in any Significant Structural Changes 3 Days after Injection"],"prefix":"10.1055","volume":"32","author":[{"given":"Morten","family":"Rose","sequence":"additional","affiliation":[{"name":"Department of Surgery, Odense University Hospital, Odense, Syddanmark, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Vermont Larner College of Medicine, Burlington, Vermont, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashton H.","family":"Pike","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Vermont Larner College of Medicine, Burlington, Vermont, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Zvara","sequence":"additional","affiliation":[{"name":"Department of Clinical Research, University of Southern Denmark, Odense, Syddanmark, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik D.","family":"Schr\u00f8der","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Odense University Hospital, Odense, Syddanmark, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva K.","family":"Hejboel","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Odense University Hospital, Odense, Syddanmark, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Qvist","sequence":"additional","affiliation":[{"name":"Department of Surgery, Odense University Hospital, Odense, Syddanmark, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark B.","family":"Elleb\u00e6k","sequence":"additional","affiliation":[{"name":"Department of Surgery, Odense University Hospital, Odense, Syddanmark, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"issue":"11","key":"ref1","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1002\/bdra.23067","article-title":"Prevalence of esophageal atresia among 18 international birth defects surveillance programs","volume":"94","author":"N Nassar","year":"2012","journal-title":"Birth Defects Res A Clin Mol Teratol"},{"issue":"03","key":"ref2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1136\/archdischild-2011-300597","article-title":"Oesophageal atresia: prevalence, prenatal diagnosis and associated anomalies in 23 European regions","volume":"97","author":"R N Pedersen","year":"2012","journal-title":"Arch Dis Child"},{"issue":"01","key":"ref3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1055\/s-0033-1333891","article-title":"The Foker technique (FT) and Kimura advancement (KA) for the treatment of children with long-gap esophageal atresia (LGEA): lessons learned at two European centers","volume":"23","author":"M Sroka","year":"2013","journal-title":"Eur J Pediatr Surg"},{"issue":"28","key":"ref4","doi-asserted-by":"crossref","first-page":"3662","DOI":"10.3748\/wjg.v18.i28.3662","article-title":"Current knowledge on esophageal atresia","volume":"18","author":"P FM Pinheiro","year":"2012","journal-title":"World J Gastroenterol"},{"issue":"06","key":"ref5","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1055\/s-0032-1331459","article-title":"Long-gap esophageal atresia: a meta-analysis of jejunal interposition, colon interposition, and gastric pull-up","volume":"22","author":"G Gallo","year":"2012","journal-title":"Eur J Pediatr Surg"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.1016\/j.jpedsurg.2013.03.062","article-title":"Intramural injection with botulinum toxin significantly elongates the pig esophagus","volume":"48","author":"H F Larsen","year":"2013","journal-title":"J Pediatr Surg"},{"issue":"03","key":"ref7","first-page":"282","article-title":"Intramural injection with botulinum toxin type A in piglet esophagus. 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