{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:35:08Z","timestamp":1793039708573,"version":"4.5.0"},"reference-count":39,"publisher":"Georg Thieme Verlag KG","issue":"03","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hibah Internal Penelitian Dosen Pemula","award":["212\/UN3\/2021"],"award-info":[{"award-number":["212\/UN3\/2021"]}]},{"DOI":"10.13039\/501100008463","name":"Universitas Airlangga","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008463","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003Bone is a dynamic tissue that undergoes remodeling. During bone remodeling, there are transcription factors such as nuclear factor-activated T cells-1 (NFATc1), sclerostin, and tartrate-resistant acid phosphatase (TRAP) that are released for bone resorption. Metabolite from gingival mesenchymal stem cells (GMSCs) has the ability to activate proliferation, migration, immunomodulation, and tissue regeneration of bone cells and tissues. Furthermore, the aim of this study is to investigate the metabolite of GMSCs' effect on expression of NFATc1, TRAP, and sclerostin in calvaria bone resorption of Wistar rats.<\/jats:p><jats:p>\n          Materials and Methods\u2003Twenty male healthy Wistar rats (Rattus norvegicus), 1 to 2 months old, 250 to 300\u2009g body were divided into four groups, namely group 1 (G1): 100 \u00b5g phosphate-buffered saline day 1 to 7; group 2 (G2): 100\u2009\u03bcg lipopolysaccharide (LPS) day 1 to 7; group 3 (G3): 100\u2009\u03bcg LPS\u2009+\u2009100\u2009\u03bcg GMSCs metabolite day 1 to 7; and group 4 (G4): 100\u2009\u03bcg GMSCs metabolite day 1 to 7. Escherichia coli LPS was used to induce inflammatory osteolysis on the calvaria with subcutaneous injection. GMSCs metabolite was collected after passage 4 to 5, then injected subcutaneously on the calvaria. All samples were sacrificed on the day 8 through cervical dislocation. The expression of TRAP, NFATc1, and sclerostin of osteoclast in the calvaria was observed with 1,000\u00d7 magnification.<\/jats:p><jats:p>\n          Statistical Analysis\u2003One-way analysis of variance and Tukey honest significant different were conducted to analyze differences between groups (p\u2009&lt;\u20090.05).<\/jats:p><jats:p>\n          Results\u2003The administration of GMSCs metabolite can significantly decrease TRAP, NFATc1, and sclerostin expression (p\u2009&lt;\u20090.05) in LPS-associated inflammatory osteolysis calvaria in Wistar rats (R. norvegicus). There were significantly different TRAP, NFATc1, and sclerostin expressions between groups (p\u2009&lt;\u20090.05).<\/jats:p><jats:p>\n          Conclusion\u2003GMSCs metabolite decrease TRAP, NFATc1, and sclerostin expression in LPS-associated osteolysis calvaria in Wistar rats (R. norvegicus) as documented immunohistochemically.<\/jats:p>","DOI":"10.1055\/s-0042-1748529","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T22:57:48Z","timestamp":1655852268000},"page":"881-888","source":"Crossref","is-referenced-by-count":8,"title":["Gingival Mesenchymal Stem Cells Metabolite Decreasing TRAP, NFATc1, and Sclerostin Expression in LPS-Associated Inflammatory Osteolysis In Vivo"],"prefix":"10.1055","volume":"17","author":[{"given":"Alexander Patera","family":"Nugraha","sequence":"additional","affiliation":[{"name":"Dental Regenerative Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"},{"name":"Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nastiti Faradilla","family":"Ramadhani","sequence":"additional","affiliation":[{"name":"Dental Regenerative Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"},{"name":"Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wibi","family":"Riawan","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igo Syaiful","family":"Ihsan","sequence":"additional","affiliation":[{"name":"Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diah Savitri","family":"Ernawati","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rini Devijanti","family":"Ridwan","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida Bagus","family":"Narmada","sequence":"additional","affiliation":[{"name":"Dental Regenerative Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"},{"name":"Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Saskianti","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fianza","family":"Rezkita","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andari","family":"Sarasati","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengku Natasha Eleena Binti Tengku Ahmad","family":"Noor","sequence":"additional","affiliation":[{"name":"Membership Faculty of Dental Surgery, Royal Collage of Surgery, Edinburgh University, 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