{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T11:21:50Z","timestamp":1767612110482},"reference-count":19,"publisher":"Scientific Scholar","issue":"03","license":[{"start":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T00:00:00Z","timestamp":1654819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Background\u2003Nucleic acid amplification testing (NAT) for the screening of blood donations is known to improve blood safety. The decision to initiate NAT requires careful deliberation of infrastructure, skilled manpower, and financial resources. This report outlines the initiative of the Government of Odisha to implement NAT screening in government blood banks in the state of Odisha, India, through public\u2013private partnership (PPP) and evaluates the incremental yield of minipool NAT screening over serology testing of blood donations.<\/jats:p><jats:p>\n          Methods\u2003Blood donations collected between June 2016 and September 2018 were initially screened for HBV (HBsAg), HCV (anti-HCV), and HIV (anti-HIV-1 and HIV-2) by ELISA, and syphilis and malaria. Sero-nonreactive donations were further screened in pools of six by Roche cobas TaqScreen MPX test version 2.0 (MPX2) NAT.<\/jats:p><jats:p>\n          Results\u2003On screening 3,39,472 blood donations, 1.34% seroreactive donations were detected. In all, 847 NAT-reactive donations (0.26%): 693 HBV, 58 HCV, and 96 HIV were detected. The NAT yields were 1:386 overall, 1:472 for HBV, 1:5642 for HCV, and 1:3409 for HIV.<\/jats:p><jats:p>\n          Conclusion\u2003NAT testing using the highly sensitive MPX2 assay leads to incremental detection of TTIs over serology. Implementation of NAT along with serological testing in blood centers all over India will be an important step towards providing safe blood. Our study not only highlights the benefits of minipool NAT testing but also presents a scalable PPP model that can serve as a template for application across other states.<\/jats:p>","DOI":"10.1055\/s-0042-1748825","type":"journal-article","created":{"date-parts":[[2022,6,11]],"date-time":"2022-06-11T00:07:44Z","timestamp":1654906064000},"page":"317-323","source":"Crossref","is-referenced-by-count":3,"title":["Adaptation of a Public\u2013Private Partnership Model for the Implementation of Minipool Nucleic Acid Testing for Screening Routine Blood Donations and Assay Evaluation"],"prefix":"10.25259","volume":"14","author":[{"given":"Smita","family":"Mahapatra","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Sri-Rama Chandra Bhanja Medical College & Hospital, Cuttack, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pankaj","family":"Parida","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Sri-Rama Chandra Bhanja Medical College & Hospital, Cuttack, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chitta Ranjan","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Veer Surendra Sai Institute of Medical Sciences and Research, Burla, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabita","family":"Palai","sequence":"additional","affiliation":[{"name":"Transfusion Medicine, Maharaja Krishna Chandra Gajapati Medical College Hospital, Berhampur, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debashis","family":"Mishra","sequence":"additional","affiliation":[{"name":"Blood Bank, Capital Hospital, Bhubaneswar, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susmita","family":"Behera","sequence":"additional","affiliation":[{"name":"Transfusion Medicine, Maharaja Krishna Chandra Gajapati Medical College Hospital, Berhampur, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabindra Kumar","family":"Mishra","sequence":"additional","affiliation":[{"name":"Transfusion Medicine, Maharaja Krishna Chandra Gajapati Medical College Hospital, Berhampur, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binayak Prasad","family":"Prusty","sequence":"additional","affiliation":[{"name":"Central Red Cross Blood Bank, Cuttack, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalyan","family":"Sarkar","sequence":"additional","affiliation":[{"name":"BMC Hospital, Bhubaneswar, Odisha, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"key":"ref1","volume-title":"A Report on Rapid Situation Assessment of Blood Transfusion Services in India\u201d","author":"National AIDS Control Organisation (NACO)","year":"2014"},{"key":"ref2","volume-title":"National Blood Transfusion Council (NBTC). Assessment of Blood Banks in India-2016","author":"National AIDS Control Organisation (NACO)"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"412105","DOI":"10.1155\/2014\/412105","article-title":"Screening donated blood for transfusion transmitted infections by serology along with NAT and response rate to notification of reactive results: an Indian experience","volume":"2014","author":"R Chaurasia","year":"2014","journal-title":"J Blood Transfus"},{"issue":"02","key":"ref4","first-page":"IR01","article-title":"Nucleic acid amplification testing (NAT): an innovative diagnostic approach for enhancing blood safety","volume":"6","author":"M Shrivastava","year":"2017","journal-title":"National Journal of Laboratory Medicine."},{"issue":"02","key":"ref5","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.transci.2015.08.003","article-title":"Confirmation and follow up of initial \u201cNAT yields\u201d: Prospective study from a tertiary healthcare center in India","volume":"54","author":"P Pandey","year":"2016","journal-title":"Transfus Apheresis Sci"},{"issue":"01","key":"ref6","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4103\/0973-6247.126679","article-title":"Nucleic acid testing-benefits and constraints","volume":"8","author":"R Hans","year":"2014","journal-title":"Asian J Transfus Sci"},{"key":"ref7","volume-title":"HBV or HCV infections via cellular blood components and plasma","author":"Guidelines On Estimation Of Residual Risk Of Hiv WHO.","year":"2016"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0166-0934(00)00283-4","article-title":"An international collaborative study to establish the 1st international standard for HIV-1 RNA for use in nucleic acid-based techniques","volume":"92(2)","author":"H Holmes","year":"2001","journal-title":"J Virol Methods"},{"issue":"01","key":"ref9","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1046\/j.1423-0410.2001.00003.x","article-title":"An international collaborative study to establish a World Health Organization international standard for hepatitis B virus DNA nucleic acid amplification techniques","volume":"80","author":"J Saldanha","year":"2001","journal-title":"Vox Sang"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.sbspro.2014.11.033","article-title":"Public private partnership in the health sector: boon or bane","volume":"157","author":"K B Thadani","year":"2014","journal-title":"Procedia Soc Behav Sci"},{"key":"ref13","volume-title":"A Report on the \u201cAssessment of Blood Banks in Odisha, India\u201d","author":"NACO","year":"2015"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"718671","DOI":"10.5402\/2012\/718671","article-title":"Need for nucleic acid testing in countries with high prevalence of transfusion-transmitted infections","volume":"2012","author":"R Jain","year":"2012","journal-title":"ISRN Hematol"},{"issue":"01","key":"ref15","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4103\/0973-6247.150938","article-title":"Automated nucleic acid amplification testing in blood banks: an additional layer of blood safety","volume":"9","author":"P Chigurupati","year":"2015","journal-title":"Asian J Transfus Sci"},{"issue":"02","key":"ref16","doi-asserted-by":"crossref","first-page":"116","DOI":"10.4103\/0973-6247.115566","article-title":"Transfusion transmittable infections - 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