{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:24:33Z","timestamp":1771374273802,"version":"3.50.1"},"reference-count":34,"publisher":"Scientific Scholar","issue":"04","license":[{"start":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T00:00:00Z","timestamp":1654473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003Non-Hodgkin lymphoma (NHL) is a common hematological malignancy. There is very little known about the expression of neuroendocrine immunohistochemical markers and their clinical significance in NHL due to the paucity of studies. Our objective was to study the expression of neuroendocrine immunohistochemical markers in NHL and correlate with clinical parameters.<\/jats:p><jats:p>\n          Materials and Methods\u2003All cases diagnosed as NHL on morphology and immunohistochemistry (World Health Organization, 2016 classification) were included in the study. Immunohistochemistry for neuron-specific enolase (NSE), synaptophysin, and chromogranin A was performed. The results were correlated with clinical parameters and response to chemotherapy.<\/jats:p><jats:p>\n          Results\u2003A total of 66 cases were included in the study with a male-to-female ratio of 3.1:1. The most frequent subtypes observed were diffuse large B-cell lymphoma not otherwise specified and follicular lymphoma. Among the neuroendocrine markers, positivity was observed only for NSE, whereas the other markers were uniformly negative. It was positive in both B- and T-cell lymphomas and in many different subtypes. No relation with the age and sex of the patients was observed. However, NSE-positive cases, more frequently, presented in the advanced stage as compared with NSE negative (61 vs. 38%). All NSE-positive cases showed remission with chemotherapy.<\/jats:p><jats:p>\n          Conclusion\u2003Among the neuroendocrine immunohistochemical markers, positivity was observed only for NSE. This isolated positivity suggests cross-binding of NSE antibodies with some other isoenzyme of NSE. NSE positivity was associated with higher stage and better response to therapy. Despite this apparent paradox, it is recommended that NSE should be part of routine immunohistochemical panel for NHL.<\/jats:p>","DOI":"10.1055\/s-0042-1748829","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T22:40:02Z","timestamp":1654555202000},"page":"427-434","source":"Crossref","is-referenced-by-count":3,"title":["Expression of Neuron-Specific Enolase and Other Neuroendocrine Markers is Correlated with Prognosis and Response to Therapy in Non-Hodgkin Lymphoma"],"prefix":"10.25259","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7863-0972","authenticated-orcid":false,"given":"Somshankar","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Pathology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.P","family":"Kataria","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit Kumar","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Pathology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2022,6,6]]},"reference":[{"issue":"S 01","key":"ref1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1159\/000447577","article-title":"Epidemiology of non-Hodgkin's lymphoma in India","volume":"91","author":"R Nair","year":"2016","journal-title":"Oncology"},{"issue":"14","key":"ref2","first-page":"4078","article-title":"Expression of neuroendocrine cell markers L-dopa decarboxylase, chromogranin A, and dense core granules in human tumors of endocrine and nonendocrine origin","volume":"48","author":"A F Gazdar","year":"1988","journal-title":"Cancer Res"},{"key":"ref3","volume-title":"The approach to metastatic endocrine tumors of unknown primary site","author":"S Hafezi-Bakthiari","year":"2016"},{"issue":"06","key":"ref4","doi-asserted-by":"crossref","first-page":"508","DOI":"10.5858\/arpa.2015-0173-CP","article-title":"Practical applications in immunohistochemistry: carcinomas of unknown primary site","volume":"140","author":"P L Kandalaft","year":"2016","journal-title":"Arch Pathol Lab Med"},{"issue":"03","key":"ref5","first-page":"458","article-title":"Detection of chromogranin in neuroendocrine cells with a monoclonal antibody","volume":"115","author":"B S Wilson","year":"1984","journal-title":"Am J Pathol"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"3500","DOI":"10.1073\/pnas.83.10.3500","article-title":"Synaptophysin: a marker protein for neuroendocrine cells and neoplasms","volume":"83","author":"B Wiedenmann","year":"1986","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"03","key":"ref7","first-page":"551","article-title":"Synaptophysin: structure of the human gene and assignment to the X chromosome in man and mouse","volume":"47","author":"T Oz\u00e7elik","year":"1990","journal-title":"Am J Hum Genet"},{"issue":"10","key":"ref8","doi-asserted-by":"crossref","first-page":"4760","DOI":"10.1073\/pnas.93.10.4760","article-title":"Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release","volume":"93","author":"H T McMahon","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"03","key":"ref9","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0167-0115(95)00069-N","article-title":"Chromogranin A: current status as a precursor for bioactive peptides and a granulogenic\/sorting factor in the regulated secretory pathway","volume":"58","author":"A L Iacangelo","year":"1995","journal-title":"Regul Pept"},{"issue":"23","key":"ref10","doi-asserted-by":"crossref","first-page":"11559","DOI":"10.1016\/S0021-9258(18)37995-X","article-title":"Molecular cloning and primary structure of human chromogranin A (secretory protein I) cDNA","volume":"263","author":"L J Helman","year":"1988","journal-title":"J Biol Chem"},{"issue":"03","key":"ref11","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/ni.1844","article-title":"Chromogranin A is an autoantigen in type 1 diabetes","volume":"11","author":"B D Stadinski","year":"2010","journal-title":"Nat Immunol"},{"issue":"01","key":"ref12","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0005-2795(74)90273-6","article-title":"Enolase isoenzymes in rat tissues. 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