{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:36:10Z","timestamp":1785929770945,"version":"3.56.0"},"reference-count":37,"publisher":"Georg Thieme Verlag KG","issue":"08","license":[{"start":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:00:00Z","timestamp":1656374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Institute for Health Research England (NIHR) for the NIHR BioResource project","award":["RG65966"],"award-info":[{"award-number":["RG65966"]}]},{"name":"National Institute for Health Research England (NIHR) for the NIHR BioResource project","award":["RG94028"],"award-info":[{"award-number":["RG94028"]}]},{"name":"National Institute for Health Research England (NIHR) for the NIHR BioResource project","award":["PI18\/00598"],"award-info":[{"award-number":["PI18\/00598"]}]},{"name":"National Institute for Health Research England (NIHR) for the NIHR BioResource project","award":["PI21\/00174"],"award-info":[{"award-number":["PI21\/00174"]}]},{"name":"National Institute for Health Research England (NIHR) for the NIHR BioResource project","award":["PMP21\/00052"],"award-info":[{"award-number":["PMP21\/00052"]}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundaci\u00f3n S\u00e9neca","award":["19873\/GERM\/15"],"award-info":[{"award-number":["19873\/GERM\/15"]}]},{"name":"Fundaci\u00f3n S\u00e9neca"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The identification of inherited antithrombin deficiency (ATD) is critical to prevent potentially life-threatening thrombotic events. Causal variants in SERPINC1 are identified for up to 70% of cases, the majority being single-nucleotide variants and indels. The detection and characterization of structural variants (SVs) in ATD remain challenging due to the high number of repetitive elements in SERPINC1. Here, we performed long-read whole-genome sequencing on 10 familial and 9 singleton cases with type I ATD proven by functional and antigen assays, who were selected from a cohort of 340 patients with this rare disorder because genetic analyses were either negative, ambiguous, or not fully characterized. We developed an analysis workflow to identify disease-associated SVs. This approach resolved, independently of its size or type, all eight SVs detected by multiple ligation-dependent probe amplification, and identified for the first time a complex rearrangement previously misclassified as a deletion. Remarkably, we identified the mechanism explaining ATD in 2 out of 11 cases with previous unknown defect: the insertion of a novel 2.4\u2009kb SINE-VNTR-Alu retroelement, which was characterized by de novo assembly and verified by specific polymerase chain reaction amplification and sequencing in the probands and affected relatives. The nucleotide-level resolution achieved for all SVs allowed breakpoint analysis, which revealed repetitive elements and microhomologies supporting a common replication-based mechanism for all the SVs. Our study underscores the utility of long-read sequencing technology as a complementary method to identify, characterize, and unveil the molecular mechanism of disease-causing SVs involved in ATD, and enlarges the catalogue of genetic disorders caused by retrotransposon insertions.<\/jats:p>","DOI":"10.1055\/s-0042-1749345","type":"journal-article","created":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T00:04:38Z","timestamp":1656461078000},"page":"1369-1378","source":"Crossref","is-referenced-by-count":26,"title":["Long-Read Sequencing Identifies the First Retrotransposon Insertion and Resolves Structural Variants Causing Antithrombin Deficiency"],"prefix":"10.1055","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6696-7762","authenticated-orcid":false,"given":"Bel\u00e9n","family":"de la Morena-Barrio","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB-Arrixaca), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), Universidad de Murcia, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Stephens","sequence":"additional","affiliation":[{"name":"Department of Haematology, NHS Blood and Transplant Centre, University of Cambridge, Cambridge, United Kingdom"},{"name":"NIHR BioResource, Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7426-4947","authenticated-orcid":false,"given":"Mar\u00eda Eugenia","family":"de la Morena-Barrio","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB-Arrixaca), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), Universidad de Murcia, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Stefanucci","sequence":"additional","affiliation":[{"name":"Department of Haematology, NHS Blood and Transplant Centre, University of Cambridge, Cambridge, United Kingdom"},{"name":"National Health Service Blood and Transplant (NHSBT), Cambridge Biomedical Campus, Cambridge, United Kingdom"},{"name":"BHF Centre of Excellence, Division of Cardiovascular Medicine, Addenbrooke's Hospital, Cambridge Biomedical Campus, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Padilla","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB-Arrixaca), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), Universidad de Murcia, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Mi\u00f1ano","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB-Arrixaca), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), Universidad de Murcia, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Gleadall","sequence":"additional","affiliation":[{"name":"Department of Haematology, NHS Blood and Transplant Centre, University of Cambridge, Cambridge, United Kingdom"},{"name":"NIHR BioResource, Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Luis","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda, Hospital Universitario de Salamanca, Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Fernanda","family":"L\u00f3pez-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda, Complexo Hospitalario Universitario de A Coru\u00f1a, A Coru\u00f1a, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Emmanuel","family":"Morange","sequence":"additional","affiliation":[{"name":"Laboratory of Haematology, La Timone Hospital, Marseille, France"},{"name":"C2VN, INRAE, INSERM, Aix-Marseille Universit\u00e9, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marja","family":"Puurunen","sequence":"additional","affiliation":[{"name":"The Framingham Heart Study, National Heart, Lung and Blood Institute, Framingham, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anetta","family":"Undas","sequence":"additional","affiliation":[{"name":"Department of Experimental Cardiac Surgery, Anesthesiology and Cardiology, Institute of Cardiology, Jagiellonian University Medical College and John Paul II Hospital, Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8089-4945","authenticated-orcid":false,"given":"Francisco","family":"Vidal","sequence":"additional","affiliation":[{"name":"Banc de Sang i Teixits, Barcelona, Spain"},{"name":"Vall d'Hebron Research Institute, Universitat Aut\u00f2noma de Barcelona (VHIR-UAB), Barcelona, Spain"},{"name":"CIBER de Enfermedades Cardiovasculares, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances Lucy","family":"Raymond","sequence":"additional","affiliation":[{"name":"NIHR BioResource, Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Cambridge, United Kingdom"},{"name":"Department of Medical Genetics, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicente","family":"Vicente","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB-Arrixaca), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), Universidad de Murcia, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem H.","family":"Ouwehand","sequence":"additional","affiliation":[{"name":"Department of Haematology, NHS Blood and Transplant Centre, University of Cambridge, Cambridge, United Kingdom"},{"name":"NIHR BioResource, Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Corral","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB-Arrixaca), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), Universidad de Murcia, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Sanchis-Juan","sequence":"additional","affiliation":[{"name":"Department of Haematology, NHS Blood and Transplant Centre, University of Cambridge, Cambridge, United Kingdom"},{"name":"NIHR BioResource, Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"NIHR BioResource","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2022,6,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"92","DOI":"10.3109\/00365516509077290","article-title":"Thrombophilia caused by inheritable deficiency of blood antithrombin","volume":"17","author":"O Egeberg","year":"1965","journal-title":"Scand J Clin Lab Invest"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.thromres.2018.07.008","article-title":"The genetics of antithrombin","volume":"169","author":"J Corral","year":"2018","journal-title":"Thromb Res"},{"issue":"21","key":"ref3","doi-asserted-by":"crossref","first-page":"5314","DOI":"10.1182\/blood-2008-10-184879","article-title":"Selective testing for thrombophilia in patients with first venous thrombosis: results from a retrospective family cohort study on absolute thrombotic risk for currently known thrombophilic defects in 2479 relatives","volume":"113","author":"W M Lijfering","year":"2009","journal-title":"Blood"},{"issue":"06","key":"ref4","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1111\/j.1538-7836.2010.03840.x","article-title":"A prospective cohort study on the absolute risks of venous thromboembolism and predictive value of screening asymptomatic relatives of patients with hereditary deficiencies of protein S, protein C or antithrombin","volume":"8","author":"B K Mahmoodi","year":"2010","journal-title":"J Thromb Haemost"},{"issue":"06","key":"ref5","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1080\/17474086.2019.1611424","article-title":"Management of antithrombin deficiency: an update for clinicians","volume":"12","author":"C Bravo-P\u00e9rez","year":"2019","journal-title":"Expert Rev Hematol"},{"issue":"02","key":"ref6","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/1479-7364-4-2-69","article-title":"The Human Gene Mutation Database: providing a comprehensive central mutation database for molecular diagnostics and personalized genomics","volume":"4","author":"P D Stenson","year":"2009","journal-title":"Hum Genomics"},{"issue":"05","key":"ref7","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1016\/j.ajhg.2016.08.022","article-title":"Structural chromosomal rearrangements require nucleotide-level resolution: lessons from next-generation sequencing in prenatal diagnosis","volume":"99","author":"Z Ordulu","year":"2016","journal-title":"Am J Hum Genet"},{"issue":"05","key":"ref8","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1055\/s-0037-1613898","article-title":"Major structural defects in the antithrombin gene in four families with type I antithrombin deficiency\u2013partial\/complete deletions and rearrangement of the antithrombin gene","volume":"83","author":"N J Beauchamp","year":"2000","journal-title":"Thromb Haemost"},{"issue":"01","key":"ref9","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/nbt.1600","article-title":"Nucleotide-resolution analysis of structural variants using BreakSeq and a breakpoint library","volume":"28","author":"H YK Lam","year":"2010","journal-title":"Nat Biotechnol"},{"issue":"01","key":"ref10","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/s13073-018-0606-6","article-title":"Complex structural variants in Mendelian disorders: identification and breakpoint resolution using short- and long-read genome sequencing","volume":"10","author":"A Sanchis-Juan","year":"2018","journal-title":"Genome Med"},{"issue":"06","key":"ref11","first-page":"779","article-title":"Long read sequencing of 1,817 Icelanders provides insight into the role of structural variants in human disease","volume":"53","author":"D Beyter","year":"2021"},{"issue":"06","key":"ref12","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1038\/s41592-018-0001-7","article-title":"Accurate detection of complex structural variations using single-molecule sequencing","volume":"15","author":"F J Sedlazeck","year":"2018","journal-title":"Nat Methods"},{"issue":"01","key":"ref13","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1038\/s41467-017-01343-4","article-title":"Mapping and phasing of structural variation in patient genomes using nanopore sequencing","volume":"8","author":"M Cretu Stancu","year":"2017","journal-title":"Nat Commun"},{"issue":"05","key":"ref14","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s00134-019-05552-x","article-title":"Whole genome sequencing reveals that genetic conditions are frequent in intensively ill children","volume":"45","author":"C E French","year":"2019","journal-title":"Intensive Care Med"},{"issue":"12","key":"ref15","doi-asserted-by":"crossref","first-page":"e1002384","DOI":"10.1371\/journal.pgen.1002384","article-title":"Repetitive elements may comprise over two-thirds of the human genome","volume":"7","author":"A PJ de Koning","year":"2011","journal-title":"PLoS Genet"},{"issue":"05","key":"ref16","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1160\/TH16-11-0866","article-title":"High levels of latent antithrombin in plasma from patients with antithrombin deficiency","volume":"117","author":"M de la Morena-Barrio","year":"2017","journal-title":"Thromb Haemost"},{"issue":"08","key":"ref17","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1111\/jth.13372","article-title":"Hypoglycosylation is a common finding in antithrombin deficiency in the absence of a SERPINC1 gene defect","volume":"14","author":"M E de la Morena-Barrio","year":"2016","journal-title":"J Thromb Haemost"},{"issue":"07","key":"ref18","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1101\/gr.244939.118","article-title":"Structural variants identified by Oxford Nanopore PromethION sequencing of the human genome","volume":"29","author":"W De Coster","year":"2019","journal-title":"Genome Res"},{"issue":"16","key":"ref19","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The sequence alignment\/map format and SAMtools","volume":"25","author":"H Li","year":"2009","journal-title":"Bioinformatics"},{"issue":"02","key":"ref20","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/s41592-019-0669-3","article-title":"Fast and accurate long-read assembly with wtdbg2","volume":"17","author":"J Ruan","year":"2020","journal-title":"Nat Methods"},{"issue":"18","key":"ref21","doi-asserted-by":"crossref","first-page":"3094","DOI":"10.1093\/bioinformatics\/bty191","article-title":"Minimap2: pairwise alignment for nucleotide sequences","volume":"34","author":"H Li","year":"2018","journal-title":"Bioinformatics"},{"issue":"01","key":"ref22","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1146\/annurev-med-100708-204735","article-title":"Structural variation in the human genome and its role in disease","volume":"61","author":"P Stankiewicz","year":"2010","journal-title":"Annu Rev Med"},{"issue":"06","key":"ref23","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: a flexible suite of utilities for comparing genomic features","volume":"26","author":"A R Quinlan","year":"2010","journal-title":"Bioinformatics"},{"issue":"20","key":"ref24","doi-asserted-by":"crossref","first-page":"3600","DOI":"10.1093\/bioinformatics\/bty350","article-title":"Snakemake-a scalable bioinformatics workflow engine","volume":"34","author":"J K\u00f6ster","year":"2018","journal-title":"Bioinformatics"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/s41586-020-2434-2","article-title":"Whole-genome sequencing of patients with rare diseases in a national health system","volume":"583","author":"E Turro","year":"2020","journal-title":"Nature"},{"issue":"06","key":"ref26","doi-asserted-by":"crossref","first-page":"R80","DOI":"10.1186\/gb-2014-15-6-r80","article-title":"SVA retrotransposon insertion-associated deletion represents a novel mutational mechanism underlying large genomic copy number changes with non-recurrent breakpoints","volume":"15","author":"J Vogt","year":"2014","journal-title":"Genome Biol"},{"issue":"12","key":"ref27","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1038\/s41576-019-0165-8","article-title":"Transposable elements in human genetic disease","volume":"20","author":"L M Payer","year":"2019","journal-title":"Nat Rev Genet"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1146\/annurev-genet-110711-155616","article-title":"Active transposition in genomes","volume":"46","author":"C RL Huang","year":"2012","journal-title":"Annu Rev Genet"},{"issue":"01","key":"ref29","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1007\/s12185-015-1765-5","article-title":"SVA retrotransposition in exon 6 of the coagulation factor IX gene causing severe hemophilia B","volume":"102","author":"Y Nakamura","year":"2015","journal-title":"Int J Hematol"},{"issue":"07","key":"ref30","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1002\/humu.22092","article-title":"Insertion of an SVA element, a nonautonomous retrotransposon, in PMS2 intron 7 as a novel cause of Lynch syndrome","volume":"33","author":"H M van der Klift","year":"2012","journal-title":"Hum Mutat"},{"issue":"05","key":"ref31","doi-asserted-by":"crossref","first-page":"897.e21","DOI":"10.1016\/j.cell.2018.02.011","article-title":"Dissecting the causal mechanism of X-linked dystonia-Parkinsonism by integrating genome and transcriptome assembly","volume":"172","author":"T Aneichyk","year":"2018","journal-title":"Cell"},{"issue":"51","key":"ref32","doi-asserted-by":"crossref","first-page":"E11020","DOI":"10.1073\/pnas.1712526114","article-title":"Disease onset in X-linked dystonia-parkinsonism correlates with expansion of a hexameric repeat within an SVA retrotransposon in TAF1","volume":"114","author":"D C Bragg","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"01","key":"ref33","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s13100-016-0065-9","article-title":"Roles for retrotransposon insertions in human disease","volume":"7","author":"D C Hancks","year":"2016","journal-title":"Mob DNA"},{"issue":"04","key":"ref34","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1056\/NEJMra1510092","article-title":"Mobile DNA in health and disease","volume":"377","author":"H H Kazazian Jr","year":"2017","journal-title":"N Engl J Med"},{"issue":"04","key":"ref35","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/nrg.2015.25","article-title":"Mechanisms underlying structural variant formation in genomic disorders","volume":"17","author":"C MB Carvalho","year":"2016","journal-title":"Nat Rev Genet"},{"issue":"02","key":"ref36","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s12185-014-1596-9","article-title":"A complex genomic abnormality found in a patient with antithrombin deficiency and autoimmune disease-like symptoms","volume":"100","author":"I Kato","year":"2014","journal-title":"Int J Hematol"},{"issue":"01","key":"ref37","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s00439-009-0742-6","article-title":"Detection and characterisation of large SERPINC1 deletions in type I inherited antithrombin deficiency","volume":"127","author":"V Picard","year":"2010","journal-title":"Hum Genet"}],"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0042-1749345.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T23:21:47Z","timestamp":1661124107000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0042-1749345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,28]]},"references-count":37,"journal-issue":{"issue":"08","published-online":{"date-parts":[[2022,8,21]]},"published-print":{"date-parts":[[2022,8]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0042-1749345","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,28]]}}}