{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:37:16Z","timestamp":1740148636406,"version":"3.37.3"},"reference-count":31,"publisher":"Georg Thieme Verlag KG","issue":"05","license":[{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Bras Ortop (Sao Paulo)"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Resumo<\/jats:title><jats:p>\n          Objetivo\u2003Testar um modelo experimental para necrose isqu\u00eamica da cabe\u00e7a femoral na doen\u00e7a de Legg-Calv\u00e9-Perthes avaliando a marcha, exames de imagens e morfohistologia.<\/jats:p><jats:p>\n          M\u00e9todos\u2003Operaram-se 11 leit\u00f5es. Induziu-se a necrose por cerclagem no colo femoral direito. Dividiram-se os leit\u00f5es em grupo A com 8 animais, sacrificando-se dois na 2\u00aa, 4\u00aa, 6\u00aa e 8\u00aa semanas, respectivamente; e grupo B, com 2 animais (sham), submetidos ao procedimento cir\u00fargico sem a cerclagem do colo femoral direito. A classifica\u00e7\u00e3o da marcha utilizada foi a de Etterlin. Os f\u00eamures congelados foram submetidos \u00e0 radiografia digital e tomografia computadorizada. Mediram-se a altura e largura da ep\u00edfise e o coeficiente epifis\u00e1rio nos tempos de estudo. Realizou-se, microscopia de luz e imunohistoqu\u00edmica com TGF-\u03b21.<\/jats:p><jats:p>\n          Resultados\u2003Um animal morreu por sepse no grupo A. Neste grupo, observou-se claudica\u00e7\u00e3o em todos os animais. Na radiografia digital e tomografia computadorizada observaram-se: esclerose \u00f3ssea, alargamento do colo femoral direito, achatamento, colapso e fragmenta\u00e7\u00e3o da cabe\u00e7a femoral direita. Todos os valores da altura da ep\u00edfise e coeficiente epifis\u00e1rio da cabe\u00e7a femoral direita foram menores que os contralaterais, nos quais observaram-se condr\u00f3citos desordenados e separados por lacunas. Observou-se redu\u00e7\u00e3o da express\u00e3o do TGF-\u03b21 com 2 e 6 semanas nas cabe\u00e7as femorais direitas e nas esquerdas com oito. No grupo B, n\u00e3o ocorreram sinais de necrose e a marcha foi normal.<\/jats:p><jats:p>\n          Conclus\u00f5es\u2003O modelo apresentado reproduziu a necrose macroscopicamente, na radiografia digital, tomografia computadorizada e microscopia. A avalia\u00e7\u00e3o da marcha demonstrou boa correla\u00e7\u00e3o com os demais achados de isquemia.<\/jats:p><jats:p>\n          N\u00edvel de Evid\u00eancia\u2003V. Estudos diagn\u00f3sticos.<\/jats:p>","DOI":"10.1055\/s-0042-1749418","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T22:50:32Z","timestamp":1665701432000},"page":"e771-e780","source":"Crossref","is-referenced-by-count":0,"title":["Padroniza\u00e7\u00e3o de modelo animal para o estudo da necrose isqu\u00eamica da cabe\u00e7a femoral na doen\u00e7a de Legg-Calv\u00e9-Perthes"],"prefix":"10.1055","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7072-5611","authenticated-orcid":false,"given":"Edson Hidenori","family":"Miashiro","sequence":"additional","affiliation":[{"name":"Disciplina de Ortopedia e Traumatologia da Faculdade de Medicina da Funda\u00e7\u00e3o Educacional do Munic\u00edpio de Assis (FEMA), Assis, S\u00e3o Paulo, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8551-7743","authenticated-orcid":false,"given":"Luis Francisco","family":"Zanella","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria da Universidade Estadual de Londrina, Londrina, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6096-1642","authenticated-orcid":false,"given":"Guilherme Schiess","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria da Universidade Estadual de Londrina, Londrina, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5742-0987","authenticated-orcid":false,"given":"Gislene dos Santos","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Cirurgia da Universidade Estadual de Londrina, Londrina, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3078-2122","authenticated-orcid":false,"given":"Kauana","family":"de Angelis","sequence":"additional","affiliation":[{"name":"Departamento de Cirurgia da Universidade Estadual de Londrina, Londrina, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2387-3264","authenticated-orcid":false,"given":"Silvio Henrique Maia","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Departamento de Cirurgia da Universidade Estadual de Londrina, Londrina, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"issue":"06","key":"ref1","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1002\/jor.20350","article-title":"Biomechanical properties of bone and cartilage in growing femoral head following ischemic osteonecrosis","volume":"25","author":"T J Koob","year":"2007","journal-title":"J Orthop 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