{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T11:58:14Z","timestamp":1781179094655,"version":"3.54.1"},"reference-count":24,"publisher":"Scientific Scholar","issue":"02","license":[{"start":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T00:00:00Z","timestamp":1661126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["South Asian J Cancer"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:p>Background\u2003Optical diagnosis is one of the upcoming methods in screening and diagnosing oral cancer at the earlier stage. Currently, DNA-based diagnosis is used along with light-based imaging methods to diagnose oral cancer rapidly.<\/jats:p>\n                  <jats:p>Aim\u2003The aim of this study was to discriminate oral cancer and oral potentially malignant disorders from normal patient with fluorescence spectroscopic characterization of salivary DNA using ethidium bromide dye.<\/jats:p>\n                  <jats:p>Materials and Methods\u2003A total of 40 patients with average age of 20 to 60 years in both the genders were selected and divided into three groups. Group A included clinically proven cases of oral cancer, group B1 included clinically diagnosed cases of leukoplakia, group B2 included clinically diagnosed cases of oral submucous fibrosis, and group C included controls. Salivary DNA fluorescence spectrum obtained after adding ethidium bromide was analyzed using FluoroLog spectrophotometer at 480\u2009nm wavelength.<\/jats:p>\n                  <jats:p>Results\u2003The discriminant analysis of fluorescent emission of salivary DNA shows predictive accuracy of 90% between group C and group A, 95% between group C and group B1, and 65% observed between group C and group B2.<\/jats:p>\n                  <jats:p>Conclusion\u2003From this study, screening of oral cancer can be done at the earliest with the help of fluorescence spectroscopic characterization of salivary DNA. This method can be done rapidly and noninvasively.<\/jats:p>","DOI":"10.1055\/s-0042-1750186","type":"journal-article","created":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T19:09:16Z","timestamp":1661195356000},"page":"159-165","source":"Crossref","is-referenced-by-count":0,"title":["Significance of Fluorescent Spectroscopy in Screening Oral Potentially Malignant Disorders and Oral Cancer by Characterization of Salivary DNA Using Ethidium Bromide\u2014A Comparative Study"],"prefix":"10.25259","volume":"12","author":[{"given":"Sadaksharam","family":"Jayachnadran","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai, Tamil Nadu, India"},{"name":"Tamil Nadu Dr. MGR Medical University, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruna","family":"Prakasarao","sequence":"additional","affiliation":[{"name":"Department of Medical Physics, College of Engineering Anna University, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangeetha","family":"Ramamoorthy","sequence":"additional","affiliation":[{"name":"Department of Dental Surgery, Government Hospital, Valangaiman, Thiruvarur District, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9242-2678","authenticated-orcid":false,"given":"Yuvaraj","family":"Manoharan","sequence":"additional","affiliation":[{"name":"Department of Medical Physics, College of Engineering Anna University, Chennai, Tamil Nadu, India"},{"name":"Department of Biochemistry, SRM Arts and Science College, Kattankulathur, Chengalpattu, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2022,8,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.oraloncology.2008.06.002","article-title":"Global epidemiology of oral and oropharyngeal cancer","volume":"45","author":"S Warnakulasuriya","year":"2009","journal-title":"Oral Oncol"},{"issue":"06","key":"ref3","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.oooo.2018.03.011","article-title":"Clinical features and presentation of oral potentially malignant disorders","volume":"125","author":"S Warnakulasuriya","year":"2018","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol"},{"key":"ref4","first-page":"11","article-title":"Raman spectroscopic analysis of blood, urine, saliva and tissue of oral potentially malignant disorders and malignancy-a diagnostic study","volume":"2","author":"S Jaychandran","year":"2016","journal-title":"Int J Oral Craniofac Sci"},{"issue":"02","key":"ref5","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s00784-018-2337-x","article-title":"Salivary biomarkers for oral cancer and pre-cancer screening: a review","volume":"22","author":"J Kaur","year":"2018","journal-title":"Clin Oral Investig"},{"issue":"08","key":"ref6","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1111\/j.1601-0825.2010.01684.x","article-title":"Optical diagnostics in the oral cavity: an overview","volume":"16","author":"p Wilder-Smith","year":"2010","journal-title":"Oral Dis"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/b978-0-323-48067-3.00012-3","volume-title":"Overview of Fluorescence Spectroscopy and Imaging for Early Cancer Detection. 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