{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T17:07:40Z","timestamp":1784740060461,"version":"3.55.0"},"reference-count":23,"publisher":"Georg Thieme Verlag KG","issue":"S 01","funder":[{"DOI":"10.13039\/501100007957","name":"Chongqing Education Commission","doi-asserted-by":"crossref","award":["KJQN201900420"],"award-info":[{"award-number":["KJQN201900420"]}],"id":[{"id":"10.13039\/501100007957","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Perinatol"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:p>\n            Objective\u2003The intestinal mucosal and immune barriers play considerable roles in the pathogenesis of necrotizing enterocolitis (NEC). The present research was designed to assess the protective effects of Lactobacillus reuteri (LR) DSM 17938 (LR 17938) on the intestinal barriers and its beneficial effects on inflammation in a neonatal mouse model of NEC.<\/jats:p><jats:p>\n            Study Design\u2003Overall, 7-day-old 75 C57BL\/6 neonatal mice were separated into three groups (n\u2009=\u200925) as follows: (1) control, (2) NEC, and (3) NEC\u2009+\u2009LR17938 (LR group). NEC mice were administered a hypertonic feeding formula and subjected to asphyxia and hypothermia. Hematoxylin and eosin (HE) staining and pathological scores were used to assess the pathological changes in the intestine. Oxidative stress was evaluated based on the levels of superoxide dismutase (SOD) and malondialdehyde (MDA). Tumor necrosis factor (TNF)-\u03b1 and interleukin (IL)-1\u03b2 levels were detected to assess inflammation. Gut permeability levels, bacterial translocation, and the levels of secretory idioglobulin A (sIgA), \u03b2-defensin, and tight junction (TJ) proteins were detected to evaluate gut mucosal and immune barrier function, and gut microbial diversity was detected to assess the composition of the gut flora.<\/jats:p><jats:p>\n            Results\u2003LR 17938 administration decreased the NEC-induced increase in intestinal scores, mortality rate, gut damage, the MDA level, and TNF-\u03b1 and IL-1\u03b2 expressions. Besides, LR 17938 improved the survival rate of NEC mice. Moreover, LR 17938 administration improved gut permeability levels, SOD activity and the bacterial translocation, ameliorated the expression of TJ proteins, and improved the gut microbiota compared with those of NEC mice.<\/jats:p><jats:p>\n            Conclusion\u2003LR 17938 reduced intestinal inflammation and played a protective role in a neonatal animal model of NEC, possibly by regulating oxidative stress and exerting a protective effect on the gut mucosal and immune barriers.<\/jats:p><jats:p>\n            Key Points\n          <\/jats:p>","DOI":"10.1055\/s-0042-1755554","type":"journal-article","created":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T00:41:37Z","timestamp":1668213697000},"page":"e386-e393","source":"Crossref","is-referenced-by-count":11,"special_numbering":"S","title":["Protective Effects of Lactobacillus reuteri on Intestinal Barrier Function in a Mouse Model of Neonatal Necrotizing Enterocolitis"],"prefix":"10.1055","volume":"41","author":[{"given":"Zhuoli","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China"},{"name":"Department of Pediatrics, Children's Hospital of Yongchuan District, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2022,11,11]]},"reference":[{"issue":"01","key":"ref1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1001\/jamapediatrics.2016.2708","article-title":"New medical and surgical insights into neonatal necrotizing enterocolitis: a review","volume":"171","author":"B L Frost","year":"2017","journal-title":"JAMA Pediatr"},{"issue":"01","key":"ref2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1053\/j.sempedsurg.2017.11.003","article-title":"Necrotizing enterocolitis: pathophysiology from a historical context","volume":"27","author":"D Hackam","year":"2018","journal-title":"Semin Pediatr Surg"},{"issue":"01","key":"ref3","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13578-021-00530-7","article-title":"Probiotics mixture reinforces barrier function to ameliorate necrotizing enterocolitis by regulating PXR-JNK pathway","volume":"11","author":"X Zhao","year":"2021","journal-title":"Cell Biosci"},{"issue":"02","key":"ref4","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1002\/jcp.25435","article-title":"LPS induces hyper-permeability of intestinal epithelial cells","volume":"232","author":"A Bein","year":"2017","journal-title":"J Cell 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