{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:39:24Z","timestamp":1785929964127,"version":"3.56.0"},"reference-count":21,"publisher":"Georg Thieme Verlag KG","issue":"S 01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Perinatol"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:p>\n            Objective\u2003The aim of this study was to present the results obtained in the Newborn Screening Program (NSP) for sickle cell disease (SCD) in western Andalusia and the autonomous city of Ceuta in the first 3 years of implementation, and to describe the discrepancies found in the diagnosis of hemoglobinopathies between the screening method and the confirmatory tests.<\/jats:p><jats:p>\n            Study Design\u2003A descriptive and retrospective study was carried out, and the findings obtained in the newborns included in the NSP between November 2018 and December 2021 were analyzed.<\/jats:p><jats:p>\n            Results\u2003A total of 111,205 samples were screened by high-performance liquid chromatography (HPLC). The birth prevalence of SCD, sickle cell trait, hemoglobin C carriers, and the compound heterozygosity Hb C\/\u03b2-thalassemia was 1\/12,356, 1\/467, 1\/1,278, and 1\/55,602 newborns, respectively. Although there was a correlation between the first-line HPLC screening technique (VARIANTnbs HPLC analyzer, Bio-Rad) and the confirmatory tests in most cases, major discrepancies were found in detecting carriers of G-Philadelphia, D, E, and O-Arab hemoglobin variants, with the former having an incidence of 1\/10,110 and the others 1\/22,241. The carrier status of Hb G-Philadelphia produced an FAD pattern on the screening method that could be mistaken as Hb D, while Hb O-Arab was identified as an FA5 pattern. Hb D was initially recognized as Hb D in two cases.<\/jats:p><jats:p>\n            Conclusion\u2003An NSP requires at least two different combined methods in order to identify the hemoglobin variant with sufficient certainty. Furthermore, even though software solutions for HPLC suggest a pattern, it must be confirmed with another technique to obtain a correct interpretation of the chromatograms.<\/jats:p><jats:p>\n            Key Points\n          <\/jats:p>","DOI":"10.1055\/s-0042-1759646","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T23:56:32Z","timestamp":1672358192000},"page":"e893-e900","source":"Crossref","is-referenced-by-count":7,"special_numbering":"S","title":["Neonatal Screening for Sickle Cell Disease in Western Andalusia: Results and Lessons Learnt after 3 Years of Implementation"],"prefix":"10.1055","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0360-5625","authenticated-orcid":false,"given":"David","family":"N\u00fa\u00f1ez-Jurado","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry Department, Virgen del Roc\u00edo University Hospital, Institute of Biomedicine of Seville (IBiS\/CSIC), Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4288-7362","authenticated-orcid":false,"given":"Salvador","family":"Pay\u00e1n-Pern\u00eda","sequence":"additional","affiliation":[{"name":"Red Blood Cell Disorders Unit, Hematology Department, Virgen del Roc\u00edo University Hospital, Institute of Biomedicine of Seville (IBiS\/CSIC), Seville, Spain"},{"name":"Medicine Department, Universitat Aut\u00f2noma de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2394-0890","authenticated-orcid":false,"given":"Ana I.","family":"\u00c1lvarez-R\u00edos","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry Department, Virgen del Roc\u00edo University Hospital, Institute of Biomedicine of Seville (IBiS\/CSIC), Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Jim\u00e9nez-Jambrina","sequence":"additional","affiliation":[{"name":"Red Blood Cell Disorders Unit, Hematology Department, Virgen del Roc\u00edo University Hospital, Institute of Biomedicine of Seville (IBiS\/CSIC), Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada","family":"Concepcion P\u00e9rez-De-Soto","sequence":"additional","affiliation":[{"name":"Red Blood Cell Disorders Unit, Hematology Department, Virgen del Roc\u00edo University Hospital, Institute of Biomedicine of Seville (IBiS\/CSIC), Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Jos\u00e9 Palma-Vallellano","sequence":"additional","affiliation":[{"name":"Hematology Department, Juan Ram\u00f3n Jim\u00e9nez University Hospital, Huelva, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roc\u00edo","family":"Zapata-Bautista","sequence":"additional","affiliation":[{"name":"Hematology Department, Juan Ram\u00f3n Jim\u00e9nez University Hospital, Huelva, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Carlos Hern\u00e1ndez-Castellet","sequence":"additional","affiliation":[{"name":"Hematology, Immunology and Genetics Department, University Hospital Puerta del Mar, C\u00e1diz, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Paz Garrastazul-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Hematology, Immunology and Genetics Department, University Hospital Puerta del Mar, C\u00e1diz, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Arqueros-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Hematology Department, University Hospital Reina Sof\u00eda, C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda del Mar","family":"Urbano-Ramos","sequence":"additional","affiliation":[{"name":"Hematology Department, University Hospital Reina Sof\u00eda, C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Abdelkader-Maanan","sequence":"additional","affiliation":[{"name":"Primary Care Medical Department, INGESA-Ceuta, Ceuta"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Ra\u00fal Garc\u00eda-Lozano","sequence":"additional","affiliation":[{"name":"Immunology Department, Virgen del Roc\u00edo University Hospital, Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3360-9245","authenticated-orcid":false,"given":"Carmen","family":"Delgado-Pecell\u00edn","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry Department, Virgen del Roc\u00edo 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