{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T21:04:18Z","timestamp":1766869458858,"version":"3.37.3"},"reference-count":29,"publisher":"Scientific Scholar","issue":"01","license":[{"start":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T00:00:00Z","timestamp":1674000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008968","name":"National Research Council of Sri Lanka","doi-asserted-by":"crossref","award":["14\u201345"],"award-info":[{"award-number":["14\u201345"]}],"id":[{"id":"10.13039\/100008968","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University of Colombo Research","award":["AP\/3\/2\/2018\/SG\/16"],"award-info":[{"award-number":["AP\/3\/2\/2018\/SG\/16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objectives\u2003This study aimed to evaluate the performance of routinely used phenotypic tests to detect \u03b2-lactamase production in isolates coproducing multiple \u03b2-lactamase types.<\/jats:p><jats:p>\n          Methods\u2003Commonly used phenotypic tests for the detection of extended spectrum \u03b2-lactamases (ESBL), AmpC \u03b2-lactamase, and carbapenemases were compared with detection and sequencing of \u03b2-lactamase genes (as the reference test) in 176 uropathogenic Enterobacteriaceae coproducing multiple \u03b2-lactamases from two hospitals in the Western Province of Sri Lanka.<\/jats:p><jats:p>\n          Results\u2003Majority of the isolates (147\/176, 83.5%) carried \u03b2-lactamase genes with (90\/147, 61%) harboring multiple genes. The Clinical and Laboratory Standards Institute screening method using cefotaxime (sensitivity [Se], 97; specificity [Sp], 93; accuracy [Ac], 94) and ceftriaxone (Se, 97; Sp, 91; Ac, 93) was the most effective to detect ESBLs. The modified double disc synergy test (Se, 98; Sp, 98; Ac, 97) and combined disc test (Se, 94; Sp, 98; Ac, 96) showed good specificity for confirmation of ESBLs. Cefoxitin resistance (Se, 97; Sp, 73; Ac, 85) and the AmpC disc test (Se, 96; Sp, 82; Ac, 86) were sensitive to detect AmpC \u03b2-lactamase producers coproducing other \u03b2-lactamases but showed low specificity, probably due to coproduction of carbapenemases. Meropenem was useful to screen for New Delhi metallo \u03b2-lactamases and OXA-48-like carbapenemases (Se, 97; Sp, 96; Ac, 96). The modified carbapenem inactivation method showed excellent performance (Se, 97; Sp, 98; Ac, 97) in identifying production of both types of carbapenemases and was able to distinguish this from carbapenem resistance due to potential mutations in the porin gene.<\/jats:p><jats:p>\n          Conclusion\u2003Microbiology laboratories that are still depend on phenotypic tests should utilize tests that are compatible with the types of \u03b2-lactamase prevalent in the region and those that are least affected by coexisting resistance mechanisms.<\/jats:p>","DOI":"10.1055\/s-0042-1760399","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T23:45:04Z","timestamp":1674085504000},"page":"117-125","source":"Crossref","is-referenced-by-count":3,"title":["Performance of Phenotypic Tests to Detect \u03b2-Lactamases in a Population of \u03b2-Lactamase Coproducing Enterobacteriaceae Isolates"],"prefix":"10.25259","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8905-9882","authenticated-orcid":false,"given":"Vindya","family":"Perera","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Microbiology, University of Colombo, Sri Lanka"},{"name":"Faculty of Medicine, Department of Microbiology, Sabaragamuwa University of Sri Lanka, Ratnapura, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3148-6731","authenticated-orcid":false,"given":"Nelun de","family":"Silva","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Microbiology, Sabaragamuwa University of Sri Lanka, Ratnapura, Sri Lanka"},{"name":"Neville Fernando Teaching Hospital, Malabe, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3931-6630","authenticated-orcid":false,"given":"Kushlani","family":"Jayatilleke","sequence":"additional","affiliation":[{"name":"Sri Jayewardenapura General Hospital, Nugegoda, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara de","family":"Silva","sequence":"additional","affiliation":[{"name":"Sri Jayewardenapura General Hospital, Nugegoda, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1450-2098","authenticated-orcid":false,"given":"Enoka","family":"Corea","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Microbiology, University of Colombo, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2023,1,18]]},"reference":[{"issue":"10","key":"ref1","doi-asserted-by":"crossref","first-page":"e0000018","DOI":"10.1371\/journal.pgph.0000018","article-title":"(2021). 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