{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T18:24:26Z","timestamp":1788805466189,"version":"build-2803163510"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"09","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inhibition of aldosterone synthase (CYP11B2) is an alternative treatment option to mineralocorticoid receptor antagonism to prevent harmful aldosterone effects. FAD286 is the best characterized aldosterone synthase inhibitor. However, to date, no study has used sensitive liquid chromatography-tandem mass spectrometry to characterize in detail the effect of FAD286 on the secreted steroid hormone profile of adrenocortical cells. Basal aldosterone production in NCI-H295R cells was detectable and 9-fold elevated after stimulation with angiotensin II. FAD286 inhibited this increase, showing a maximal effect at 10\u2009nmol\/l. Higher concentrations of FAD286 did not further reduce aldosterone concentrations, but showed a parallel reduction in corticosterone, cortisol and cortisone levels, reflecting additional inhibition of steroid-11\u03b2-hydroxylase (CYP11B1). Pregnenolone, progesterone and 17-OH-progesterone levels remained unaffected. In conclusion, the aldosterone synthase inhibitor FAD286 lowers angiotensin II-induced aldosterone concentrations in adrenocortical cells but the relative lack of selectivity over CYP11B1 is evident at higher FAD286 concentrations.<\/jats:p>","DOI":"10.1055\/s-0043-113829","type":"journal-article","created":{"date-parts":[[2017,7,31]],"date-time":"2017-07-31T22:34:25Z","timestamp":1501540465000},"page":"701-706","source":"Crossref","is-referenced-by-count":6,"title":["Impact of Aldosterone Synthase Inhibitor FAD286 on Steroid Hormone Profile in Human Adrenocortical Cells"],"prefix":"10.1055","volume":"49","author":[{"given":"Coy","family":"Brunssen","sequence":"additional","affiliation":[{"name":"Division of Vascular Endothelium and Microcirculation, Department of Medicine III, Medical Faculty Carl Gustav Carus and University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Division of Vascular Endothelium and Microcirculation, Department of Medicine III, Medical Faculty Carl Gustav Carus and University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirko","family":"Peitzsch","sequence":"additional","affiliation":[{"name":"Division of Clinical Neurochemistry, Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Frenzel","sequence":"additional","affiliation":[{"name":"Division of Vascular Endothelium and Microcirculation, Department of Medicine III, Medical Faculty Carl Gustav Carus and University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Ziegler","sequence":"additional","affiliation":[{"name":"Paul Langerhans Institute Dresden of the Helmholtz-Zentrum M\u00fcnchen at the Medical Faculty Carl Gustav Carus and University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"},{"name":"German Center for Diabetes Research e.V., Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Brown","sequence":"additional","affiliation":[{"name":"Cardio Metabolic Diseases, Boehringer Ingelheim Pharmaceuticals Inc, Ridgefield, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Weldon","sequence":"additional","affiliation":[{"name":"Cardio Metabolic Diseases, Boehringer Ingelheim Pharmaceuticals Inc, Ridgefield, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme","family":"Eisenhofer","sequence":"additional","affiliation":[{"name":"Division of Clinical Neurochemistry, Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"},{"name":"Department of Medicine III, University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Willenberg","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Rostock University Medical Center, Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Bornstein","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"},{"name":"Endocrinology and Diabetes, Division of Diabetes & Nutritional Sciences, Rayne Institute, Denmark Hill Campus, Faculty of Life Sciences & Medicine, Kings College London, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Morawietz","sequence":"additional","affiliation":[{"name":"Division of Vascular Endothelium and Microcirculation, Department of Medicine III, Medical Faculty Carl Gustav Carus and University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"},{"name":"Department of Medicine III, University Hospital Carl Gustav Carus Dresden, Technische Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2017,7,31]]},"container-title":["Hormone and Metabolic Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0043-113829.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,12,19]],"date-time":"2017-12-19T11:13:07Z","timestamp":1513681987000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0043-113829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,31]]},"references-count":0,"journal-issue":{"issue":"09","published-online":{"date-parts":[[2017,9,8]]},"published-print":{"date-parts":[[2017,9]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0043-113829","relation":{},"ISSN":["0018-5043","1439-4286"],"issn-type":[{"value":"0018-5043","type":"print"},{"value":"1439-4286","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,31]]}}}